Alternation of plasma membrane H+-ATPase in cucumber roots under different iron nutrition

• Autorzy: Mlodzinska E.
• Języki publikacji: EN

Abstrakty

EN

The strategy I developed by dicotyledonous plants to efficiently acquire of iron involve the action of proton-extruding H⁺-ATPases. These proteins are responsible for the solubilization of iron through rhizosphere acidification. Previously, it has been documented that acidification of the rhizosphere involve specific isoforms of plasma membrane proton pumps in response to Fe deficiency in dicot plants such as Arabidopsis, Cucumis sativus, Lycopersicon and Pisum sativum. In this work, we have investigated the effect of Fe nutritional status on the activity of plasma membrane H⁺-ATPases at the level of transcription, protein accumulation and post-translational modification. Two genes (CsHA2 and CsHA3) were isolated from different parts of cucumber and their expression analyzed under different Fe treatments (0, 25 and 80 µM Fe). Transcript level of CsHA2 was detected in both vegetative organs (roots and cotyledons), while CsHA3 expression was limited only to the roots in immature plants. These two genes were up-regulated under Fe deficiency in cucumber roots and their expression was decreased in the presence of 80 µM Fe. We have shown that the post-translational modification of protein via phosphorylation and its accumulation as a target of activation in Fe-deficient plants might not be responsible for the increase in the H⁺-ATPase activity.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2125-2133,fig.,ref.

Twórcy

autor

Mlodzinska E.

• Department of Plant Physiology, Institute of Experimental Biology, Kanonia 6/8, 50-328 Wroclaw, Poland

Bibliografia

• Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118
• Arango M, Gevaudant F, Oufattole M, Boutry M (2003) The plasma membrane proton pump ATPase: the significance of gene subfamilies. Planta 216:355–365
• Bauer P, Ling HQ, Guerinot ML (2007) FIT, the FER-like iron deficiency induced transcription factor in Arabidopsis. Plant Physiol Biochem 45(5):260–271
• Borch J, Bych K, Roepstorff P, Palmgren MG, Fuglsang AT (2002) Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H⁺-ATPase. Biochem Soc Trans 30:411–415
• Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principles of protein dye binding. Anal Biochem 72:248–254
• Brumbarova T, Bauer P (2008) Iron uptake and transport in plants. Plant membrane and vacuolar transporters. In: Jaiwal PK, Maharshi Dayanand, Singh RP, Baba Saheb Bhimrao Ambedkar, Dhankher OP (eds) Plant membrane and vacuolar transporters. CAB International
• Burzyński M, Migocka M, Kłobus G (2005) Cu and Cd transport in cucumber (Cucumis sativus L.) root plasma membranes. Plant Sci 168:1609–1614
• Colangelo EP, Guerinot ML (2004) The essential basic helix-loophelix protein FIT1 is required for the iron deficiency response. Plant Cell 16:3400–3412
• Dell’Orto M, Santi S, De Nisi P, Cesco S, Varanini Z, Zocchi G, Pinton R (2000) Development of Fe-deficiency responses in cucumber (Cucumis sativus L.) roots: involvement of plasma membrane H⁺ATPase activity. J Exp Bot 51(345):695–701
• Duby G, Boutry M (2008) The plant plasma membrane proton pump ATPase: a highly regulated P-type ATPase with multiple physiological roles. Pflugers Arch Eur J Physiol. doi:10.1007/s00424-008-0457-x
• Enomoto Y, Hodoshima H, Shimada H, Shoji K, Yoshihara T, Goto F (2007) Long-distance signals positively regulate the expression of iron uptake genes in tobacco roots. Planta 227(1):81–89
• Fox TC, Guerinot ML (1998) Molecular biology of cation transport in plants. Annu Rev Plant Physiol Plant Mol Biol 49:669–696
• Fuglsang AT, Cuin TA, Guo Y, Qiu Q, Song Ch, Kristiansen KA, Bych K, Schulz A, Shabala S, Schumaker KS, Palmgren MG, Zhub JK (2007) Arabidopsis protein kinase PKS5 inhibits the plasma membrane H1-ATPase by preventing interaction with 14-3-3 protein. Plant Cell 19:1617–1634
• Gallagher SR, Leonard RT (1982) Effect of vanadate, molybdate and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots. Plant Physiol 70:1335–1340
• Haruta M, BurchHL, NelsonRB, Barrett-Wilt G, Kline KG,Mohsin SB, Young JC, Otegui MS, Sussman RM (2010) Molecular characterization of mutant Arabidopsis plants with reduced plasma membrane proton pump activity. J Biol Chem 258:17918–17929
• Hodge A (2004) The plastic plant: root responses to heterogeneous supplies of nutrients. New Phytol 162:9–24
• Huang S, Li R, Zhang Z et al (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41:1275–1281
• Janicka-Russak M, Kłobus G (2007) Modification of plasma membrane and vacuolar H⁺ ATPases in response to NaCl and ABA. J Plant Physiol 164:295–302
• Jeong J, Connolly EL (2009) Iron uptake mechanisms in plants: function of the FRO family of ferric reductases. Plant Sci. doi: 10.1016/j.plantsci.2009.02.011
• Jeong J, Guerinot ML (2009) Homing in on iron homeostasis in plants. Trends Plant Sci 14(5):280–285
• Kim SA, Guerinot ML (2007) Mining iron: Iron uptake and transport in plants. FEBS Lett 581:2273–2280
• Kłobus G (1995) The role of plasma membrane-bound activities in nitrate transport into sealed plasma membrane vesicles form Cucumis sativus L. roots. In: Baluska F et al (ed) Developments in plant soil science. Structure and function of roots. Kluwer Academic Publishers, Dordrecht
• Kłobus G, Buczek J (1995) The role of plasma membrane oxidoreductase activity in proton transport. J Plant Physiol 146:103–107
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
• Larsson C (1985) Plasma membranes. In: Jackson JF, Linskens HF (eds) Modern methods of plant analysis, vol 1. Cell components. Springer, Berlin 1985
• Ling HQ, Du J, WangN(2009) Progress in understanding the molecular regulation of I uptake in strategy I plants. In: Bañuelos GS, Li ZQ (eds) Development and uses of biofortified agricultural products. Taylor and Francis Group, CRC Press, Boca Raton
• Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press Inc., San Diego
• Młodzińska E, Wdowikowska A, Kłobus G (2010) Identification and characterization of two genes encoding plasma membrane H⁺-ATPase in Cucumis sativus L. Acta Physiol Plant 32:1103–1111
• Morsomme P, Boutry M (2000) The plant plasma membrane H(+)- ATPase: structure, function and regulation. Biochim Biophys Acta 1456:1–16
• Palmgren M (2001) Plant plasma membrane H⁺-ATPases: powerhouses for nutrient uptake. Annu Rev Plant Phys Plant Mol Biol 52:817–845
• Rabotti G, Zocchi G (1994) Plasma membrane-bound H⁺-ATPase and reductase activities in Fe-deficient cucumber roots. Physiol Plant 90:779–785
• Sairam RK, Rao KV, Srivastava GC (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037–1046
• Santi S, Schmidt W (2008) Laser microdissection-assisted analysis of the functional fate of iron deficiency-induced root hairs in cucumber. J Exp Bot 59(3):697–704
• Santi S, Locci G, Monte R, Pinton R, Varanini Z (2003) Induction of nitrate uptake in maize roots: expression of a putative highaffinity nitrate transporter and plasma membrane H⁺-ATPase isoforms. J Exp Bot 54:1851–1864
• Santi S, Cesco S, Varanini Z, Pinton R (2005) Two plasma membrane H⁺ATPase genes are differentially expressed in iron-deficient cucumber plants. Plant Physiol Biochem 43:287–292
• Schikora A, Schmidt W (2002) Formation of transfer cells and H⁺-ATPase expression in tomato roots under P and Fe deficiency. Planta 215:304–311
• Schmidt W, Michalke W, Schikora A (2003) Proton pumping by tomato roots. Effect of Fe deficiency and hormones on the activity and distribution of plasma membrane H⁺-ATPase in rhizodermal cells. Plant Cell Environ 26(3):361–370
• Shen H, Chen J, Wang Z, Yang C, Sasaki T, Yamamoto Y, Matsumoto H, Yan X (2006) Root plasma membrane H1-ATPase is involved in the adaptation of soybean to phosphorus starvation. J Exp Bot 57:1353–1362
• Walker LE, Connolly EL (2008) Time to pump iron: iron-deficiencysignaling mechanisms of higher plants. Curr Opin Plant Biol 11(5):530–535
• Waters BM, Lucena C, Romera FJ, Jester GG, Wynn AN, Rojas CL, Alcántara E, Pérez-Vicente R (2007) Ethylene involvement in the regulation of the H⁺-ATPase CsHA1 gene and of the new isolated ferric reductase CsFRO1 and iron transporter CsIRT1 genes in cucumber plants. Plant Physiol Biochem 5:293–301
• Zocchi G (2006) Metabolic changes in iron-stressed dicotyledonous plants. In: Barton LL, Abadia J (eds) Iron nutrition in plants and rhizospheric microorganism. Springer, Berlin, pp 359–379
• Zocchi G, De Nisi P, Dell’Orto M, Espen L, Galina PM (2007) Iron deficiency differently affects metabolic responses in soybean roots. J Exp Bot 58:993–1000
• Zou C, Shen J, Zhang F, Guo S, Rengel Z, Tang C (2001) Impact of nitrogen form on iron uptake and distribution in maize seedlings in solution culture. Plant Soil 235:143–149

Uwagi

Rekord w opracowaniu