Overexpression of aldolase A and cytokeratin 19 in ovine pulmonary adenocarcinoma

• Autorzy: Kycko A. , Reichert M.
• Języki publikacji: EN

Abstrakty

Ovine pulmonary adenocarcinoma (OPA) is a transmissible lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV). In the present study the protein profiles of five neoplastic and three non-neoplastic sheep lung tissues were examined for the identification of proteins overexpressed in ovine pulmonary adenocarcinoma. Lung sections of the experimental group of sheep were collected during necropsies for proteomic and immunohistochemical examination. Two dimensional electrophoresis (2DE) was performed using gel strips with immobilized pH gradient 3-10. As a result of 2DE gel analysis 14 spots characterized by over 2-fold higher expression in tumour proteomes were selected for mass spectrometry. In eleven spots more than one polypeptide was identified indicating overlapping of proteins in gels. In two spots demonstrating over 3-fold higher expression in OPA proteomes, single proteins: cytokerarin 19 (CK19) and aldolase A were identified. Immunohistochemical studies revealed that CK19 and aldolase A were expressed in the cytoplasm of epithelial cells of bronchioles in non-neoplastic lung sections, as well as epithelial cells of bronchioles and neoplastic cells in lung sections of OPA affected sheep. The results indicate that the overexpression of the two proteins reflects the presence of neoplastic cells in the lungs of OPA affected sheep.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 4
• Opis fizyczny: p.703-709,fig.,ref.

Twórcy

autor

Kycko A.

• Department of Pathology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland

autor

Reichert M.

• Department of Pathology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

• Alix-Panabicres C, Vendrell JP, Slijper M, Pelle O, Barbotte E, Mercier G, Jacot W, Fabbro M, Pantel K (2009) Full-length cytokeratin-19 is released by human tumor cells: apotential role in metastatic progression of breast cancer. Breast Cancer Res 11: R39.
• Asaka M, Kimura T, Meguro T, Kalo M, Kudo M, Miyazaki T, Alpert E (1994) Alteration of aldolase isozymes in serum and tissues of patients with cancer and other diseases. J Clin Lab Anal 8: 144-148.
• Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovařík J, Lukáš Z, Vojtěšek B (1986) Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J 18: 565-575.
• Bates J, Rutherford R, Divilly M, Finn J, Grimes H, O’Muircheartaigh I, Gilmartin JJ (1997) Clinical value of CYFRA 21.1, carcinoembryonic antigen, neurone-specific enolase, tissue polypeptide specific antigen and tissue polypeptide antigen in the diagnosis of lung cancer. Eur Respir J 10: 2535-2538.
• Bongers V, Braakhuis BJ, Snow GB (1995) Circulating fragments of cytokeratin 19 in patients with head and neck squamous cell carcinoma. Clin Otolaryngol Allied Sci 20: 479-482.
• Chyczewski L, Nikliński J, Chyczewska E, Laudański J, Furman M (1997) Immunohistochemical analysis of tissue localization of cytokeratin 19 in lung cancer. Rocz Akad Med Bialymst 42: 162-172.
• De las Heras M, Gonzalez L, Sharp JM (2003) Pathology of ovine pulmonary adenocarcinoma. Curr Top Microbiol Immunol 275: 25-54.
• De las Heras M, Ortin A, Salvatori D, Perez de Villareal M, Cousens C, Ferrer LM, Cebrian LM, Garcia de Jalon JA, Gonzalez L, Sharp JM (2005) A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions. Res Vet Sci 79: 259-264.
• DeMartini JC, Carlson JO, Leroux C, Spencer T, Palmarini M (2003) Endogenous retroviruses related to jaagsiekte sheep retrovirus. Curr Top Microbiol Immunol 275: 117-137.
• Gefflaut T, Blonski C, Perie J, Willson M (1995) Class I aldolases: substrate specificity, mechanism, inhibitors and structural aspects. Prog Biophys Mol Biol 63: 301-340.
• Gharib TG, Chen G, Wang H, Huang CC, Prescott MS, Shedden K, Misek DE, Thomas DG, Giordano TJ, Taylor JM, Kardia S, Yee J, Orringer MB, Hanash S, Beer DG (2002) Proteomic analysis of cytokeratin isoforms uncovers association with survival in lung adenocarcinoma. Neoplasia 4: 440-448.
• Granville CA, Dennis PA (2005) An overview of lung cancer genomics and proteomics. Am J Respir Cell Mol Biol 32: 169-176.
• Gure AO, Altorki NK, Stockert E, Scanlan MJ, Old LJ, Chen YT (1998) Human lung cancer antigens recognized by autologous antibodies: definition of a novel cDNA derived from the tumor suppressor gene locus on chromosome 3p21.3. Cancer Res 58: 1034-1041.
• Hatzfeld A, Feldmann G, Guesnon J, Frayssinet C, Schapira F, Szajnert MF, Maurice M, WolfelspergerM (1978) Location of adult and fetal aldolases A, B, and C by immunoperoxidase technique in LF fast-growing rat hepatomas. Cancer Res 38: 16-22.
• Kosacka M, Jankowska R (2007). The prognostic value of cytokeratin 19 expression in non-small cell lung cancer. Pneumonol Alergol Pol 75: 317-323.
• Kreuder J, Borkhardt A, Repp R, Pekrun A, Göttsche B, Gottschalk U, Reichmann H, Schachenmayr W, Schlegel K, Lampert F (1996) Brief report: inherited metabolic myopathy and hemolysis due to a mutation in aldolase A. New Engl J Med 334: 1100-1104.
• Kusakabe T, Motoki K, Hori K (1997) Mode of interactions of human aldolase isozymes with cytoskeletons. Arch Biochem Biophys 344: 184-193.
• Kycko A, Reichert M (2008) Two-dimensional electrophoretic analysis of polypeptides expressed by normal and ovine pulmonary adenocarcinoma affected lung tissues. Bull Vet Inst Pulawy 52: 37.
• Kycko A, Reichert M (2010) PCR-based methods for detection of JSRV in experimentally and naturally infected sheep. Bull Vet Inst Pulawy 54: 445-450.
• Lódi C, Szabó E, Holczbauer A, Batmunkh E, Szíjártó A, Kupcsulik P, Kovalszky I, Paku S, Illyés G, Kiss A, Schaff Z (2006) Claudin-4 differentiates biliary tract cancers from hepatocellular carcinomas. Mod Pathol 19: 460-469.
• Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31: 11-24.
• Mornex JF, Thivolet F, De las Heras M, Leroux C (2003) Pathology of human bronchioloalveolar carcinoma and its relationship to the ovine disease. Curr Top Microbiol Immunol 275: 225-248.
• Naseem N, Reyaz N, Nagi AH, Ashraf M, Sami W (2010) Immunohistochemical expression of cytokeratin-19 in non small cell lung carcinomas – an experience from a tertiary care hospital in Lahore. Int J Pathol 8: 54-59.
• Ohshio G, Imamura T, Okada N, Yamaki K, Suwa H, Imamura M, Sakahara H (1997) Cytokeratin 19 fragment in serum and tissues of patients with pancreatic diseases. Int J Pancreatol 21: 235-241.
• Ojika T, Imaizumi M, Abe T, Kato K (1991) Immunochemical and immunohistochemical studies on three aldolase isozymes in human lung cancer. Cancer 67: 2153-2158.
• Paine R, Gaposchkin D, Kelly C, Wilcoxen SE (1995) Regulation of cytokeratin expression in rat lung alveolar epithelial cells in vitro. Am J Physiol 269: L536-L544.
• Palmarini M, Fan H (2001) Retrovirus-induced ovine pulmonary adenocarcinoma, an animal model for lung cancer. J Natl Cancer Inst 93: 1603-1614.
• Platt JA, Kraipowich N, Villafane F, DeMartini JC (2002) Alveolar type II cells expressing jaagsiekte sheep retrovirus capsid protein and surfactant proteins are the predominant neoplastic cell type in ovine pulmonary adenocarcinoma. Vet Pathol 39: 341-352.
• Pujol JL, Molinier O, Ebert W, Daurés JP, Barlesi F, Buccheri G, Paesmans M, Quoix E, Moro-Sibilot D, Szturmowicz M, Brechot JM, Muley T, Grenier J (2004) CYFRA 21-1 is a prognostic determinant in non-small-cell lung cancer: results of a meta-analysis in 2063 patients. Br J Cancer 90: 2097-2105.
• Rosadio RH, Sharp JM, Lairmore MD, Dahlberg JE, De Martini JC (1988) Lesions and retroviruses associated with naturally occurring ovine pulmonary carcinoma (sheep pulmonary adenomatosis). Vet Pathol 25: 58-66.
• Schapira F, Hatzfeld A, Reuber MD (1971) Fetal pattern of aldolase transplantable hepatomas. Cancer Res 31: 1224-1230.
• Sharp JM, DeMartini JC (2003) Natural history of JSRV in sheep. Curr Top Microbiol Immunol 275: 55-79.
• Srinivas PR, Verma M, Zhao Y, Srivastava S (2002) Proteomics for cancer biomarker discovery. Clin Chem 48: 1160-1169.
• Takashi M, Zhu Y, Nakano Y, Miyake K, Kato K (1992) Elevated levels of serum aldolase A in patients with renal cell carcinoma. Urol Res 20: 307-311.
• Tochio T, Tanaka H, Nakata S, Hosoya H (2010) Fructose-1,6-bisphosphate aldolase A is involved in HaCaT cell migration by inducing lamellipodia formation. J Dermatol Sci 58: 123-129.
• Tomonaga T, Matsushita K, Yamaguchi S, Oh-Ishi M, Kodera Y, Maeda T, Shimada H, Ochiai T, Nomura F (2004) Identification of altered protein expression and post-translational modifications in primary colorectal cancer by using agarose two-dimensional gel electrophoresis. Clin Cancer Res 10: 2007-2014.
• van Sprundel RG, van den Ingh TS, Desmet VJ, Katoonizadeh A, Penning LC, Rothuizen J, Roskams T, Spee B (2010) Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours. Comp Hepatol 9: 4.
• Wieskopf B, Demangeat C, Purohit A, Stenger R, Gries P, Kreisman H, Quoix E (1995) Cyfra 21-1 as a biologic marker of non-small cell lung cancer. Evaluation of sensitivity, specificity, and prognostic role. Chest 108: 163-169.
• York DF, Querat G (2003) A history of ovine pulmonary adenocarcinoma (jaagsiekte) and experiments leading to the deduction of the JSRV nucleotide sequence. Curr Top Microbiol Immunol 275: 1-23

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