PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 32 | 5 |

Tytuł artykułu

Somatic hybridization between the diploids of S. 3 michoacanum and S. tuberosum

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Interspecific somatic hybrids between a diploid potato clone DG 81-68 susceptible to Phytophthora infestans (Mont.) de Bary and a resistant diploid tuber-bearing species Solanum × michoacanum were generated and analyzed. About 30 regenerants displaying an intermediate morphology were obtained as a result of three separate PEG-mediated fusion experiments. The RAPD analysis confirmed the hybridity of all the regenerants. About 50% of the hybrid plants exhibited vigorous growth and were stable in culture, while the rest of them rooted poorly and grew slowly in vitro. Most of the hybrid clones were at the tetraploid level (70%), while 30% of the clones examined were at the hexaploid level. The S. × michoacanum (+) DG 81-68 hybrids with growth anomalies were aneuploid. The variation in late blight resistance of the hybrid clones was found in detached leaflet tests, with enhanced resistance characteristic for three tetraploid hybrids.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

32

Numer

5

Opis fizyczny

p.867-873,fig.,ref.

Twórcy

  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawin´skiego 5A, 02-106 Warsaw, Poland
autor
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawin´skiego 5A, 02-106 Warsaw, Poland
autor
  • The Emil Chroboczek Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
autor
  • Młocho´w Research Center, Plant Breeding and Acclimatization Institute (IHAR), Platanowa 19, 05-831 Młocho´w, Poland
autor
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawin´skiego 5A, 02-106 Warsaw, Poland

Bibliografia

  • Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R (2007) Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tiss Org Cult 91:179–189
  • Bołtowicz D, Szczerbakowa A, Wielgat B (2005) RAPD analysis of the interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum. Cell Mol Biol Lett 10:151–162
  • Debnath SC, Wenzel G (1987) Selection of somatic fusion products in potato by hybrid vigour. Potato Res 30:371–380
  • Greplová M, Polzerová H, Vlastniková H (2008) Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum. Acta Physiol Plant 30:787–796
  • Hawkes JG (1990) The potato: evolution, biodiversity and genetic resources. Belhaven Press, Washington, DC
  • Hein H, Birch PRJ, Danan S, Lefebvre V, Odeny DA, Gebhardt C, Trognitz F, Bryan GJ (2009) Progress in mapping and cloning qualitative and quantitative resistance against Phytophthora infestans in potato and its wild relatives. Potato Res 52:215–227
  • Helgeson JP, Pohlman JD, Austin S, Haberlach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RM, Stevenson WK (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742
  • Jakuczun H, Wasilewicz-Flis I (2004a) New sources of potato resistance to Phytophthora infestans at the diploid level. Plant Breed Seed Sci 50:137–145
  • Jakuczun H, Wasilewicz-Flis I (2004b) Ziemniak diploidalny źródłem cech jakościowych w hodowli. Zesz Prob Nauk Roln 500:127–136 (in Polish)
  • Jakuczun H, Strzelczyk-Żyta D, Narkiewicz M (1997) Zastosowanie pośrednich metod oznaczania poziomu ploidalności ziemniaka. Biul Inst Ziemn 48:91–98 (in Polish)
  • Kamoun S, Smart CD (2005) Late blight of potato and tomato in the genomics era. Plant Dis 89:692–699
  • Kamoun S, Huitema E, Vleeshouwers VGAA (1999) Resistance for oomycetes: a general role for the hypersensitive response? Trends Plant Sci 4:196–200
  • Laferriere LT, Helgeson JP, Callen C (1999) Fertile Solanum tuberosum + Solanum commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theor Appl Genet 98:1272–1278
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2001) Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines. Mol Gen Genom 265:694–704
  • Novy RG, Gillen AM, Whitworth JL (2007) Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum tuberosum. Theor Appl Genet 114:1161–1172
  • Preiszner J, Feler A, Veisz O, Sutka J, Dudits D (1991) Characterization of morphological variation and cold resistance in interspecific somatic hybrids between potato (Solanum tuberosum L.) and Solanum brevidens Phil. Euphytica 57:37–49
  • Serraf I, Sihachakr D, Ducreux G, Brown SC, Allot M, Barghi N, Rossignol L (1991) Interspecific somatic hybridization in potato by protoplast electrofusion. Plant Sci 76:115–126
  • Song J, Bradeen JM, Naess SK, Raasch JA, Wielgus SM, Haberlach JT, Liu J, Kuang H, Austin-Phillips S, Buell CR, Helgeson JP, Jiang J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. PNAS 100:9128–9133
  • Spooner DM, Hijmans RJ (2001) Potato systematics and germplasm collecting, 1989–2000. Am J Potato Res 78:237–268
  • Szczerbakowa A, Borkowska M, Wielgat B (2000) Plant regeneration from the protoplasts of Solanum tuberosum, S. nigrum and S. bulbocastanum. Acta Physiol Plant 22:3–10
  • Szczerbakowa A, Bołtowicz D, Wielgat B (2003) Interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum H8105. Acta Physiol Plant 25:365–373
  • Szczerbakowa A, Bołtowicz D, Lebecka R, Radomski P, Wielgat B (2005) Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105. Acta Physiol Plant 27:317–325
  • Tan MYA, Hutten RCB, Celis C, Park TH, Niks RE, Visser RGF, van Eck HJ (2008) The RPi-mcd1 locus from Solanum microdontum involved in resistance to Phytophthora infestans, causing a delay in infection, maps on potato chromosome 4 in a cluster of NBSLRR genes. Mol Plant Microbe Interact 21:909–918
  • Tek AL, Walter R, Helgeson JP, Jiang J (2004) Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato. Theor Appl Genet 109:249–254
  • Thieme R, Darsow U, Gavrilenko T, Dorokhov D, Tiemann H (1997) Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica 97:189–200
  • Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T (2008) Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet 116:691–700
  • Valkonen JPT, Rokka VM (1998) Combination and expression of two virus resistance mechanisms in interspecific somatic hybrids of potato. Plant Sci 131:85–94
  • van der Vossen EAG, Sikkema A, Hekkert BL, Gros J, Stevens P, Muskens M, Wouters D, Pereira A, Stiekema W, Allefs S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant J 36:867–882
  • Zarzycka H (2001) Evaluation of resistance to Phytophthora infestans in detached leaflet assay. Preparation of the inoculum. In: The methods of evaluation and selection applied in potato research and breeding. Monografie i rozprawy naukowe IHAR, 10a:75–77

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-508e539f-45ee-42a8-a915-f97bb572352e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.