Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon

• Autorzy: Palus K. , Rytel L.
• Języki publikacji: EN

Abstrakty

EN

Cocaine- and amphetamine-regulated transcript peptide (CART) is a substance, which can play the role of neuromediator and/or neuromodulator in nerve structures within the gastrointestinal tract. However knowledge concerning its functions and co-localisation with other neuronal factors is rather scarce. During the present investigation the co-localisation of CART and vasoactive intestinal polypeptide (VIP) in the neurons of meyenteric plexus within the porcine transverse colon was studied using double immunofluorescence technique and semiquantitative arbitrary scale of the frequency of presence CART+/VIP+, CART+/VIP– and CART–/VIP+ neuronal cells. The most often (+++) CART–/VIP+ neurons were encountered, neurons simultaneously immunoreactive to CART and VIP were observed somewhat rarer (++) and only single (+) CART+/VIP– perikarya were visible. The present study reports for the first time on the co-localisation of CART and VIP in myenteric neurons of the porcine transverse colon. (Folia Morphol 2013; 72, 4: 328–332)

Słowa kluczowe

EN

co-localization; cocaine; amphetamine; regulated transcript peptide; vasoactive intestinal polypeptide; myenteric plexus; porcine transverse colon; immunofluorescence; pig

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2013
• Tom: 72
• Numer: 4
• Opis fizyczny: p.328-332,fig.,ref.

Twórcy

autor

Palus K.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Rytel L.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

• 1. Andersson PO (1984) Vascular control in the colon and rectum. Scan J Gastroenterol Suppl, 93: 67–78.
• 2. Arciszewski MB, Sand E, Ekblad E (2008) Vasoactive intestinal peptide rescues cultured rat myenteric neurons from lipopolysaccharide induced cell death. Regul Pept, 146: 218–223.
• 3. Bellinger DL, Lorton D, Brouxhon S, Felten S, Felten DL (1996) The significance of vasoactive intestinal polypeptide (VIP) in immunomodulation. Adv Neuroimmunol, 6: 5–27.
• 4. Brenneman DE, Philips TM, Hauser J, Hill JM, Spong CY, Gozes I (2003) Complex array of cytokines released by vasoactive intestinal peptide. Neuropeptides, 37: 111–119.
• 5. Burleigh DE, Banks MR (2007) Stimulation of intestinal secretion by vasoactive intestinal peptide and cholera toxin. Auton Neurosci Bas Clin, 133: 64–75.
• 6. Ekblad E, Kuhar MJ, Wierup N, Sundler F (2003) Cocaineand amphetamine-regulated transcript distribution and function in rat gastrointestinal tract. Neurogastroenterol Motil, 15: 545–557.
• 7. Furness JB (2000) Types of neurons in the enteric nervous system. J Auton Nerv Syst, 81: 87–96.
• 8. Gonkowski S, Calka J (2010) Changes in the somatostatin (SOM)-like immunoreactivity within nervous structures of the porcine descending colon under various pathological factors. Exp Mol Pathol, 88: 416–423.
• 9. Gonkowski S, Calka J (2012) Changes in pituitary adenylate cyclase-activating peptide 27-like immunoreactive nervous structures in the porcine descending colon during selected pathological processes. J Mol Neurosci, 48: 777–787.
• 10. Gonkowski S, Kaminska B, Bossowska A, Korzon M, Landowski P, Majewski M (2003) The influence of experimental Bacteroides fragilis infection on substance P and somatostatin-immunoreactive neural elements in the porcine ascending colon: a preliminary report. Folia Morphol, 62: 455–457.
• 11. Gonkowski S, Burlinski P, Calka J (2009) Proliferative enteropathy (PE)-induced changes in galanin-like immunoreactivity in the enteric nervous system of the porcine distal colon. Acta Vet Beograd, 59: 321–330.
• 12. Gonkowski S, Kaminska B, Landowski P, Skobowiat C, Burlinski P, Majewski M, Calka J (2009) A population of zinc transporter 3-like immunoreactive neurons is present in the ganglia of human descending colon. Adv Clin Eexp Med, 18: 243–248.
• 13. Gonkowski S, Burlinski P, Skobowiat C, Majewski M, Arciszewski MB, Radziszewski P, Calka J (2009) Distribution of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) nerve structures in the porcine large intestine. Acta Vet Hung, 57: 509–520.
• 14. Gonkowski S, Kaminska B, Burlinski P, Kroll A, Calka J (2009) The influence of drug-resistant ulcerative colitis on the number of cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) mucosal nerve fibres of the descending colon in children. Przegl Gastroenterol, 4: 147–151.
• 15. Gonkowski S, Burlinski P, Skobowiat C, Majewski M, Calka J (2010) Inflammation- and axotomy-induced changes in galanin-like immunoreactive (GAL-LI) nerve structures in the porcine descending colon. Acta Vet Hung, 58: 91–103.
• 16. Gonkowski S, Burlinski P, Szwajca P, Calka J (2012) Changes in cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) nerve structures of the porcine descending colon during proliferative enteropathy. Bull Vet Inst Pul, 56: 199–203.
• 17. Gunnarsdóttir A, Wierup N, Larsson LT, Kuhar MJ, Ekblad E (2007) CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung’s disease. Eur J Pediatr Surg, 17: 184–189.
• 18. Henning RJ, Sawmiller DR (2001) Vasoactive intestinal peptide: cardiovascular effects. Card Res, 49: 27–37.
• 19. Kasparek MS, Fatima J, Iqbal CW, Duenes JA, Sarr MG (2007) Role of VIP and Substance P in NANC innervation in the longitudinal smooth muscle of the rat jejunuminfluence of extrinsic denervation. J Surg Res, 141: 22–30.
• 20. Kristensen P, Judg ME, Thim L, Ribel U, Christjansen KN, Wulff BS, Clausen JT, Jensen PB, Madsen OD, Vrang N, Larsen PJ, Hastrup S (1998) Hypothalamic CART is a new anorectic peptide regulated by leptin. Nature, 393: 72–76.
• 21. Majewski M, Bossowska A, Gonkowski S, Wojtkiewicz J, Brouns I, Scheuermann DW, Adriaensen D, Timmermans JP (2002) Neither axotomy nor target-tissue inflammation changes the NOS- or VIP-synthesis rate in distal bowel-projecting neurons of the porcine inferior mesenteric ganglion (IMG). Folia Histochem Cytobiol, 40: 151–152.
• 22. Okumura T, Yamada H, Motomura W, Kohgo Y (2000) Cocaine- amphetamine-regulated transcript (CART) acts in the central nervous system to inhibit gastric acid secretion via brain corticotrophin-releasing factor system. Endocrinology, 141: 2854–2860.
• 23. Skobowiat C, Gonkowski S, Calka J (2010) Phenotyping of sympathetic chain ganglia (SChG) neurons in porcine colitis. J Vet Med Sci, 72: 1269–1274.
• 24. Spiess J, Villarreal J, Wale W (1981) Isolation and sequence analysis of a somatostatin-like polypeptide from ovine hypothalamus. Biochemistry, 20: 1982–1988.
• 25. Tebbe JJ, Ortmann E, Schumacher K, Mönnikes H, Kobelt P, Arnold R, Schäfer MK (2004) Cocaine- and amphetamine-regulated transcript stimulates colonic motility via central CRF receptor activation and peripheral cholinergic pathways in fed conscious rats. Neurogastroenterol Motil, 16: 489–496.
• 26. Wierup N, Gunnarsdóttir A, Ekblad E, Sundler F (2007) Characterisation of CART-containing neurons and cells in the porcine pancreas, gastro-intestinal tract, adrenal and thyroid glands. BMC Neurosci, 8: 1471–2202.
• 27. Wojtkiewicz J, Gonkowski S, Bladowski M, Majewski M (2012) Characterization of cocaine and amphetamineregulated transcript-like immunoreactive (CART-LI) enteric neurons in the porcine small intestine. Acta Vet Hung, 60: 371–881.
• 28. Wojtkiewicz J, Gonkowski S, Rowniak M, Crayton R, Majewski M, Jalynski M (2012) Neurochemical characterization of zinc transporter 3-like immunoreactive (ZnT3(+)) neurons in the intramural ganglia of the porcine duodenum. J Mol Neurosci, 48:766–776.
• 29. Wojtkiewicz J, Rowniak M, Gonkowski S, Crayton R, Majewski M, Robak A, Bialkowska J, Barczewska M (2012) Proliferative enteropathy (PE)-induced changes in the calbindin-immunoreactive (CB-IR) neurons of inferior mesenteric ganglion supplying the descending colon in the pig. J Mol Neurosci, 48: 757–765.