Comparison of different molecular methods for detection of Mycoplasma gallisepticum

• Autorzy: Domanska-blicharz K. , Tomczyk G. , Minta Z.
• Języki publikacji: EN

Abstrakty

EN

An "In house" PCR method was compared with three commercial PCR kits for the detection of Mycoplasma gallisepticum (MG) in cultures grown in a modified Frey broth or agar, in vaccines, as well as in tracheal swabs of SPF chickens infected experimentally. The studied methods showed different specificity (poor specificity of commercial A test) and sensitivity. The "in house" method appeared to be more sensitive (7 fg/µL) than the rest of the tests (70 fg/µL). The "in house" PCR method could differentiate between TS-11 and 6/85 vaccine strains, also in the combination with restriction enzyme length polymorphism (RFLP). Furthermore, distinguishing between TS-11 and pathogenic field strains was also possible. To detect MG in tracheal swabs, SPF chickens were inoculated intranasally with 1x10⁶ colony forming unit (cfu)/mL of ATCC reference MG strain. Tracheal samples were collected 4, 7, 14, 28 and 35 d post infection (d.p.i) and examined with PCR-based methods. The MG strain was detected for the longest time, up to 28 d.p.i., by the "in house" method and commercial kit B, with a stronger result being obtained by the "in house" method. The results showed the usefulness of the studied methods for the MG direct detection in chicken swabs; however, some discrepancies were noted with regard to their different specificity and sensitivity.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.529-532,fig.,ref.

Twórcy

autor

Domanska-blicharz K.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Tomczyk G.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Minta Z.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

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