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2013 | 57 | 3 |

Tytuł artykułu

In vitro cultivation and immunostaining of Lawsonia intracellularis strains

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was also performed. Large number of S-shaped, moving bacteria were found in the cell medium in cultures infected with ATCC 55783 and vaccine strain. Immunostaining revealed a high number of multiple cell-associated or intracellular red stained bacteria in the infected cultures. This study describes for the first time in vitro cultivation of L. intracellularis in Poland, which creates further perspective for more advanced research on this bacterium.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

57

Numer

3

Opis fizyczny

p.319-322,fig.,ref.

Twórcy

  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1. Biester H.E., Schwarte L H.: Intestinal adenoma in swine. Am J Pathol 1931, 7, 175-185.
  • 2. Dittmar M., Hoelzle L.E., Hoelzle K., Sydler T., Corboz L., Miserez R., Wittenbrink M.M.: Diagnosis of porcine proliferative enteropathy: detection of Lawsonia intracellularis by pathological examinations, polymerase chain reaction and cell culture inoculation. J Vet Med B 2003, 50, 332-338.
  • 3. Draganov M., Murdjeva M., Michailova-Topalska T.: McCoy and McCoy-Plovdiv cell lines in experimental and diagnostic practice - past, present and perspectives. J Cult Collect 2004 2005, 4, 3-16.
  • 4. Guedes R.M.C., Gebhart C.J.: Comparison of intestinal mucosa homogenate and pure culture of the homologous Lawsonia intracellularis isolate in reproducing proliferative enteropathy in swine. Vet Microbiol 2003, 93, 159-166.
  • 5. Guedes R.M.C., Gebhart C.J.: Onset and duration of shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis. Vet Microbiol 2003, 91, 135-145.
  • 6. Huerta B., Arenas A., Carrasco L., Maldonado A., Tarradas C., Carbonero A., Perea A.: Comparison of diagnostic techniques for porcine proliferative enteropathy (Lawsonia intracellularis infection). J Comp Pathol 2003, 129, 179-185.
  • 7. Hwang J.M., Lee J.H., Yeh J.Y.: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures. BMC Res Notes 2012, 5, 78, doi: 10.1186/1756-0500-5-78.
  • 8. Jacobson M., Fellström C., Jensen-Wam M.: Porcine proliferative enteropathy: an important disease with questions remaining to be solved. Vet J 2010, 184, 264-268. doi: 10.1016/ j.tvjl.2009.05.010.
  • 9. Jensen T.K., Boesen H.T., Vigre H., Boye M.: Detection of Lawsonia intracellularis in formalin fixed porcine intestinal tissue samples: comparison of immunofluorescence and in-situ hybridization, and evaluation of the effects of controlled autolysis. J Comp Pathol 2010, 142, 1-8.
  • 10. Knittel P.J., Larson D.I., Harris D.L., Roof M.B., McOrist S.: United States isolates of Lawsonia intracellularis from porcine proliferative enteropathy resemble European isolates. Swine Health Prod 1996, 4, 119-122.
  • 11. Koyama T., Hirai T., Nagai S.: In vitro cultivation and partial characterization of Lawsonia intracellularis from a Japanese filed case of porcine proliferative enteropathy. J Vet Med Sci 2006, 68, 609-613.
  • 12. Lawson G.H.K., Gebhart C.J.: Proliferative enteropathy. J Comp Pathol 2000, 122, 77-100.
  • 13. Lawson G.H.K., Mc Orist S., Jasni S., Mackie R.A.: Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro. J Clin Microbiol 1993, 31, 1136-1142.
  • 14. McOrist S., Boid R., Lawson G.H.K., McConell I.: Monoclonal antibodies to intracellular Campylobacter-like organisms of porcine proliferative enteropathies. Vet Rec 1987, 121, 421-422.
  • 15. Nogueira M.G., Collins A.M., Donahoo M., Emery D.: Immunological responses to vaccination following experimental Lawsonia intracellularis virulent challenge in pigs. Vet Microbiol 2013, 164. 131-138, doi: 10.1016/j.vetmic.2013. 02.004.
  • 16. Pedersen K.S., Holyoake P., Stege H., Nielsen J.P.: Diagnostic performance of different fecal Lawsonia intracellularis-specific polymerase chain reaction assays as diagnostic tests for proliferative enteropathy in pigs: a review. J Vet Diagn Invest 2010, 22, 487-494.
  • 17. Smith D.G.E., Lawson G.H.K.: Lawsonia intracellularis: getting inside the pathogenesis of proliferative enteropathy. Vet Microbiol 2001, 82, 331-345.
  • 18. Vanucci F.A., Wattanaphansak S., Gebhart C.J.: An alternative method for cultivation of Lawsonia intracellularis. J Clin Microbiol 2012, 50, 1070-1072.
  • 19. Watarai M., Yoshiya M., Sato A., Furoka H.: Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig. J Vet Med Sci 2008, 70, 731-733.
  • 20. Yeh J.Y., Kim T.J., Park S.Y., Song C.S., Yoon Y.D., Kim S.K., Lee J.B., Choi I.S.: Isolation of Lawsonia intracellularis in Korea and reproduction of proliferative enteropathy in pigs and hamsters. J Vet Med Sci 2005, 68, 499-501.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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