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2009 | 53 | 3 |

Tytuł artykułu

Application of multiplex PCR for the evaluation of the occurrence of ail, ystA, and ystB genes in Yersinia enterocolitica strains isolated from wild boars (Sus scrofa)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study aimed at the evaluation of the occurrence of genes directly connected with pathogenicity in Yersinia enterocolitica (Y. enterocolitica) strains isolated from wild boars, obtained in the 2007/2008 hunting season in the North-East territory of Poland. Multiplex PCR, used for αil, ystA, and ystB gene detection, was optimised in order to determine the existence of the genes in one reaction. Forty-six palatine tonsils, gained from 46 wild boars of various ages, were preliminary examined from the bacteriological, sero- and biotyping points of view; then bacterial DNA was isolated and multiplex PCR was performed. The presence of the product of volume corresponding to the ystB gene fragment was found in two cases, which constituted 4.35% of the examined samples. The ail and ystA genes were not detected in any of the tested samples. The molecularly confirmed existence of Y. enterocolitica in wild boar palatine tonsils, indicates the carrier state and possibly shedding of the microorganism into the environment, and suggests that wild boars, likewise pigs to a lesser extent, may constitute a reservoir of the bacteria and a potential source of infection for the man.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

53

Numer

3

Opis fizyczny

p.351-355,fig.,ref.

Twórcy

  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
autor
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

Bibliografia

  • 1. Al Dahouk S., Nockler K., Tomaso H., Splettstroesser W.D., Jungersen G., Riber U., Petry T., Hoffmann D., Scholz H.C., Hensel A., Neubauer H.: Seroprevalence of brucellosis, tularemia and yersiniosis in wild boar (Sus scrofa) from North-Eastern Germany. J Vet Med B 2005, 52, 444-455.
  • 2. Gierczyński R.: The evaluation of the usefulness of selected virulence markers for the identification of virulent Yersinia enterocolitica strains. III. The chromosome encoded markers. Med Dośw Mikrobiol 2000, 52, 51-65.
  • 3. Gill C.O.: Microbiological conditions of meats from large game animals and birds. Meat Sci 2007, 77, 149- 160.
  • 4. Gliński Z., Kostro K.: Jersinioza - choroba odzwierzęca, której ważnym rezerwuarem i źródłem zakażenia jest trzoda chlewna. Trzoda Chlewna 2001, 5, 100-104.
  • 5. Grant T., Bennett-Wood V., Robins-Browne R.M.: Identification of virulence-associated characteristics in clinical isolates of Yersinia enterocolitica lacking classical virulence markers. Infect Immun 1998, 66, 1113-1120.
  • 6. Grant T., Bennet-Wood V., Robins-Browne R.M.: Characterization of the interaction between Yersinia enterocolitica biotype 1A and phagocytes and epithelial cells in vitro. Infect Immun 1999, 67, 4367-4375.
  • 7. Harnett N., Lin Y.P., Krishnan C.: Detection of pathogenic Yersinia enterocolitica using the multiplex polymerase chain reaction. Epidemiol Infect 1996, 117, 59-67.
  • 8. Jagielski M., Rastawicki W., Kałużewski S., Gierczyński R.: Yersiniosis - unappreciated infectious diseases. Przegl Epidemiol 2002, 56, 57-64.
  • 9. Jakubczak A., Kleczkowski M.: Yersinia enterocolitica u bydła - zagrożenie dla zdrowia ludzi. Magazyn Wet 2001, 54, 5-7.
  • 10. Koppel C., Knopf L., Ryser M.-P., Miserez R., Thur B., Stark K.D.C.: Serosurveillance for selected infectious disease agents in wild boars (Sus scrofa) and outdoor pigs in Switzerland. Eur J Wildl Res 2007, 53, 212-220.
  • 11. Koronkiewicz A., Daczkowska-Kozon E., Markiewicz K., Wojciechowska A., Żmuda E., Dąbrowski W.: Game animals as carriers of enteric pathogens. Folia Univ Agric Stetin, Scientia Alimentaria 2004, 238, 79-84.
  • 12. Kot B., Trafny E.A., Jakubczak A.: Application of multiplex PCR for monitoring colonization of pig tonsils by Yersinia enterocolitica, including biotype 1A, and Yersinia pseudotuberculosis. J Food Prot 2007, 70, 1110- 1115.
  • 13. Mielczarek P., Bagłaj M.: Yersiniosis - rarely diagnosed disease of gastrointestinal system. Gastroenterol Pol 2004, 11, 69-74.
  • 14. Platt-Samoraj A., Bancerz-Kisiel A., Szweda W.: Yersinia enterocolitica pathogenicity and the significance of biotype 1A in the pathogenesis of yersiniosis. Medycyna Wet 2006, 62, 1113-1115.
  • 15. Platt-Samoraj A., Ugorski M., Szweda W., Szczerba- Turek A., Wojciech Ł., Procajło Z.: Analysis of the presence of αil, ystA and ystB genes in Yersinia enterocolitica strains isolated from aborting sows and aborted fetuses. J Vet Med B 2007, 53, 341-346.
  • 16. Revell P.A., Miller V.L.: Yersinia virulence: more than a plasmid. FEMS Microbiol Lett 2001, 205, 159-164.
  • 17. Singh I., Bhatnagar S., Virdi J.S.: Isolation and characterization of Yersinia enterocolitica from diarrhoeic human subjects and other sources. Current Sci 2003, 84, 1353-1355.
  • 18. Singh I., Virdi J.S.: Production of Yersinia stable toxin (YST) and distribution of yst genes in biotype 1A strains of Yersinia enterocolitica. J Med Microbiol 2004, 53, 1065-1068.
  • 19. Singh I., Virdi J.S.: Interaction of Yersinia enterocolitica biotype 1A strains of diverse origin with cultured cells in vitro. Jpn J Infect Dis 2005, 58, 31-33.
  • 20. Tennant S.M., Grant T.H., Robins-Browne R.M.: Pathogenicity of Yersinia enterocolitica biotype 1A. FEMS Microbiol Lett 2003, 38, 127-137.
  • 21. Tennant S.M., Skinner N.A., Joe A., Robins-Browne R.M.: Homologues of insecticidal toxin complex genes in Yersinia enterocolitica biotype 1A and their contribution to virulence. Infect Immun 2005, 73, 6860-6867.
  • 22. Wiśniewski J., Bielecki J.: Virulence mechanisms of bacteria from the Yersinia genus. Post Mikrobiol 1996, 35, 213-241.
  • 23. Woźniak-Kosek A., Kot B., Jakubczak A., Grzybowski J.: Application of PCR for the identification of pathogenic Yersinia enterocolitica strains isolated from humans and pigs. Medycyna Wet 2001, 57, 183-186.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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