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2011 | 14 | 4 |

Tytuł artykułu

Construction of a reporter system for Lactobacillus sp. using the gfpuv gene

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Lactobacilli are bacteria commonly associated with the gastrointestinal tract of animals and humans. They are able to produce antimicrobial substances such as bacteriocins, lactic acid and hydrogen peroxide, the factors which have been shown to be beneficial for controlling overgrowth of potentially pathogenic bacteria. For this reason lactobacilli are often applied in probiotics. The aim of present study was to construct a reporter strain based on the gfpuv expression system which can bee used as a strain with the ability to colonize the intestinal tract in future experiments.

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Wydawca

-

Rocznik

Tom

14

Numer

4

Opis fizyczny

p.655-657,fig.,ref.

Twórcy

autor
  • Division of Bacteriology and Molecular Biology, Department of Preclinical Science, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW) Ciszewskiego 8,02-786 Warsaw, Poland
autor
autor

Bibliografia

  • Chin HS, Shim JS, Kim JM, Yang R, Yoon SS (2001) Detection and Antibacterial Activity of Bacteriocin Produced by Lactobacillus plantarum. Food Sci Biotech 10: 335-341.
  • Felis GE, Dellaglio F (2007) Taxonomy of Lactobacilli and Bifidobacteria. Curr Issues Intest Microbiol 8: 44-61.
  • Gory L, Montel MC, Zagorec M (2001) Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products. FEMS Microbiol Lett 194: 127-133.
  • O'Sullivan DJ, Klaenhammer TR (1993) Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp. Appl Environ Microbiol 59: 2730-2733.
  • Perez-Arellano I, Perez-Martinez G (2003) Optimization of the green fluorescent protein (GFP) expression from a lactose-inducible promotor in Lactobacillus casei. FEMS Microbiol Lett 222: 123-127.
  • Sambrook J, Russell DW (2001) Molecular cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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