Higher-order nuclear structure in epileptogenesis

• Autorzy: Szczepankiewicz A. , Walczak A. , Szczepinska T. , Broszkiewicz M. , Pawlowski K. , Wilczynski G.
• Języki publikacji: EN

Abstrakty

EN

Although the molecular mechanisms of gene-expression in neurons are comprehensively described in the literature, little is known about the relationship between these processes and the architecture of the neuronal cell nucleus. For example, it has never been examined whether bursts of transcriptional activity associated with seizures involve any regulation at the level of higher-order nuclear structure. Accordingly, we have performed studies on the structure of neuronal nucleus in epileptic animals. Using electron microscopy, we found the striking appearance of large interchromatin granule clusters (IGCs) in epileptic nuclei. We confi rmed this observation by immunofl uorescence-confocal analysis of IGC marker, a spliceosome assembly factor SC-35. Interestingly, there was also aggregation of spots immunoreactive for phophorylated and acetylated Histone H3, a marker of transcriptionally active chromatin. The fi ndings are consistent with the results of bioinformatic analysis of transcription profi ling in the rat kainate-induced status epilepticus (public gene expression data), showing tendency for coordinated expression of positional gene-clusters along chromosomes. Taken together, our results suggest that upon epileptogenesis there is prominent reorganization of neuronal nucleus, putatively involving formation of molecular factories, where transcription, splicing, and export of pre-mRNA are orchestrated.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.335

Twórcy

autor

Szczepankiewicz A.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Walczak A.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Szczepinska T.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Broszkiewicz M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Pawlowski K.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Wilczynski G.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland