Glutamine improves shoot morphogenesis in chickpea (Cicer arietinum L.)

• Autorzy: Patil G. , Patel R. , Jaat R. , Pattanayak A. , Jain P. , Srinivasan R.
• Języki publikacji: EN

Abstrakty

EN

The use of glutamine has been shown to increase the frequency of organogenesis and regeneration in the in vitro culture of several plants. The effect of glutamine on hormone-induced multiple shoot formation in desi and kabuli genotypes of chickpea (C-235 and PUSA-1053) were evaluated. Embryo axes with or without attached cotyledons were cultured in thidiazuron (TDZ) or 6-benzylaminopurine (BAP)-containing medium, respectively, with various concentrations of glutamine. Glutamine improved and prolonged the multiple shoot regeneration ability of the embryo axis. Chickpea embryo axis with attached cotyledon and cultured in TDZ-containing medium showed improved and prolonged shoot regeneration with 5 mM glutamine, while embryo axis without cotyledon and cultured in BAP-containing medium showed prolonged regeneration ability in 10 mM glutamine. Glutamine, however, did not serve as a substitute for cotyledon. Desi genotype (C-235) showed better response for multiple shoot formation as compared to the kabuli genotype (PUSA-1053). Glutamine at a concentration of 5 mM also improved root formation in excised in vitro shoots.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 5
• Opis fizyczny: p.1077-1084,fig.,ref.

Twórcy

autor

Patil G.

• National Research Centre on Plant Biotechnology, New Dehli, India

autor

Patel R.

• National Research Centre on Plant Biotechnology, New Dehli, India
• Navasari Agricultural University, Navasari, India

autor

Jaat R.

• National Research Centre on Plant Biotechnology, New Dehli, India

autor

Pattanayak A.

• National Research Centre on Plant Biotechnology, New Dehli, India
• ICAR Research Complex, Umiam, Meghalaya, India

autor

Jain P.

• National Research Centre on Plant Biotechnology, New Dehli, India

autor

Srinivasan R.

• National Research Centre on Plant Biotechnology, New Dehli, India

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