Genetic characterization and phylogenetic analysis of Fasciola species based on ITS2 gene sequence, with first molecular evidence of intermediate Fasciola from water buffaloes in Aswan, Egypt

• Autorzy: Omar M.A. , Elmajdoub L.O. , Ali A.O. , Ibrahim D.A. , Sorour S.S. , Al-Wabel M.A. , Ahmed A.I. , Suresh M. , Metwally A.M.
• Języki publikacji: EN

Abstrakty

EN

Fasciolosis is an important food and water-borne parasitic infection caused by the two trematode species, Fasciola hepatica, and F. gigantica. The present study aimed to identify the phenotypic features and genetic characterization of adult fasciolid that infecting buffaloes were studied in Aswan, Egypt. The genetic identity of Fasciola species was investigated by the analysis of forward and reverse sequences of the ITS-2 of the rDNA gene. The Fasciola isolates were obtained from sheep, buffaloes, and cows in the regions of Aswan. The sequence of ITS2 gene isolates obtained from the present investigation were compared with GenBank reference sequences of F. hepatica, F. gigantica, and intermediate Fasciola. The obtained results were based on morphometric and genetic data which revealed the existence of F. gigantica, F. hepatica, and an intermediate form of Fasciola. Several variable sites were encountered among the investigated isolates in the Aswan, that were compared with the Fasciola species acquiesced in Gene Bank. Furthermore, the relationships between Egyptian Fasciola and Fasciola spp. from various other nations were discussed in the study.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2021
• Tom: 67
• Numer: 1
• Opis fizyczny: p.55-65,fig.,ref.

Twórcy

autor

Omar M.A.

• Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt
• Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51452 Qassim, Saudi Arabia

autor

Elmajdoub L.O.

• Zoology Department, College of Science, Misurata University, Misurata, Libya

autor

Ali A.O.

• Division of Infectious Diseases, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, 83523, Qena Egypt

autor

Ibrahim D.A.

• Directorate of Veterinary Medicine, Aswan, Egypt

autor

Sorour S.S.

• Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Egypt

autor

Al-Wabel M.A.

• Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51452 Qassim, Saudi Arabia

autor

Ahmed A.I.

• Department of Poultry Diseases, College of Veterinary Medicine, South Valley University, Qena 83523, Egypt

autor

Suresh M.

• Department of Biology, College of Science, Al-Zulfi, Majmaah University, Riyadh Region, Majmaah 11952, Saudi Arabia

autor

Metwally A.M.

• Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt

Identyfikatory

DOI

10.17420/ap6701.312