A computational approach to structural properties of glycoside hydrolase family 4 from bacteria

• Autorzy: Craciun D. , Vlad-Oros B. , Filimon N. , Ostafe V. , Isvoran A.
• Języki publikacji: EN

Abstrakty

EN

Structural bioinformatics approaches applied to the alpha- and beta-glycosidases from the GH4 enzyme family reveal that, despite low sequence identity, these enzymes possess quite similar global structural characteristics reflecting a common reaction mechanism. Locally, there are a few distinctive structural characteristics of GH4 alpha- and beta-glycosidases, namely, surface cavities with different geometric characteristics and two regions with highly dissimilar structural organizations and distinct physicochemical properties in the alpha- and beta-glucosidases from Thermotoga maritima. We suggest that these structurally dissimilar regions may be involved in specific protein-protein interactions and this hypothesis is sustained by the predicted distinct functional partners of the investigated proteins. Also, we predict that alpha- and beta-glycosidases from the GH4 enzyme family interact with difenoconazole, a fungicide, but there are different features of these interactions especially concerning the identified structurally distinct regions of the investigated proteins.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 4
• Opis fizyczny: p.553-564,fig.,ref.

Twórcy

autor

Craciun D.

• West University of Timisoara, Teacher Training Department, Timisoara, Romania

autor

Vlad-Oros B.

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania
• Laboratory of Advanced Researches in Environmental Protection, Timisoara, Romania
• University „Al. I. Cuza” from Lasi, Lasi, Romania (temporally affiliated)

autor

Filimon N.

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania
• Laboratory of Advanced Researches in Environmental Protection, Timisoara, Romania

autor

Ostafe V.

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania
• Laboratory of Advanced Researches in Environmental Protection, Timisoara, Romania

autor

Isvoran A.

• West University of Timisoara, Department of Biology and Chemistry, Timisoara, Romania
• Laboratory of Advanced Researches in Environmental Protection, Timisoara, Romania

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