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2014 | 54 | 3 |

Tytuł artykułu

Studies on Aspergillus flavus Link. isolated from maize in Iran

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The Aspergillus flavus population structure from maize kernels was examined. During 2011, samples were collected from two main grain maize production areas in Iran (Fars and Ardebil provinces), shortly before harvest. One-hundred nine A. flavus isolates were recovered on Dichloran Rose Bengal Chloramphenicole (DRBC) agar and Aspergillus flavus/parasiticus medium (AFPA) and grouped into morphotypes and Vegetative Compatibility Groups (VCGs) based on morphological (e.g. sclerotia production), physiological (e.g. aflatoxin-producing ability) and genetic criteria (e.g. heterokaryosis). In general, morphotype and VCG composition were highly dissimilar in both provinces. In total, 43.8% and 44.3% of A. flavus isolates from Ardebil and Fars, respectively, produced sclerotia. Sclerotia producers were identified as A. flavus L and S strain morphotypes in Ardebil (66.7% and 33.3%, respectively) and Fars (29.6% and 70.4%, respectively). Furthermore, 71 isolates (65.1%) were able to produce aflatoxin (Ardebil 40.8%, Fars 59.2%). The aflatoxin values were categorized into four different classes (< 10, 10-100, 100-1,000 and > 1,000 ppb). In total, 51 aflatoxin producing isolates of A. flavus (Ardebil n = 22, Fars n = 29) were assigned into 26 VCGs by complementation of nit auxotrophs on nitrate medium. None of the A. flavus isolates from Ardebil complemented with any isolates from Fars. Genetic diversity of A. flavus isolates was 59.1% and 41.8% for Ardebil and Fars, respectively. The different geographical adaptation and genetic make-up of A. flavus isolates may be due to different climatic conditions, soil types and crop sequences in both maize production areas.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

54

Numer

3

Opis fizyczny

p.218-224,fig.,ref.

Twórcy

  • Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948974 Iran
autor
  • Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948974 Iran
  • Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948974 Iran
  • Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948974 Iran
  • Department of Agricultural Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948974 Iran
autor
  • The University of Arizona, School of Plant Sciences, Tucson 85721, USA

Bibliografia

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  • Abedi-Tizaki M., Sabbagh S.K. 2011. Fungi associated with harvested corn grains of Golestan province in Iran. Ann. Biol. Res. 2 (5): 681-688.
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  • Muthomi J.W., Mureithi B.K., Chemining G.N., Gathumbi J.K., Mutit E.W. 2012. Aspergillus species and aflatoxin B1 in soil, maize grain and flour samples from semi-arid and humid regions of Kenya. Int. J. Agri. Sci. 2 (1): 22-34.
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  • Papa K.E. 1986. Heterokaryon incompatibility in Aspergillus flavus. Mycologia 78 (1): 98-101.
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  • Razzaghi-Abyaneh M., Shams-Ghahfarokhi M., Allameh A., Kazeroon-Shiri A., Ranjbar-Bahadori S., Mirzahoseini H., Rezaee B. 2006. A survey on distribution of Aspergillus section Flavi in corn field soils in Iran: population patterns based on aflatoxin, cyclopiazonic acid and sclerotia production. Mycopathologia 161 (3): 183-192.
  • Sefidgar S.A.A., Mirzae M., Assmar M., Naddaf S.R. 2011. Aflatoxin M1 in pasteurized milk in Babol city, Mazandaran Province, Iran. Iranian J. Public Health 40 (1): 115-118.
  • Shephard G.S., Marasas W.F., Yazdanpanah H., Rahimian H., Safavi N., Zarghi A., Shafaati A., Rasekh H.R. 2002. Fumonisin B1 in maize harvested in Iran during 1999. Food Addit. Contam. 19 (4): 676-679.
  • Yazdanpanah H., Miraglia M., Calfapietra F.R., Brera C. 2001a. Natural occurrence of aflatoxins and ochratoxin A in corn and barley from Mazandaran and Golestan in north provinces of Iran. Mycotoxin Res. 17 (1): 21-30.
  • Yazdanpanah H., Miraglia M., Calfapietra F.R., Brera C., Rasekh H.R. 2001b. Natural occurrence of mycotoxins in cereals from Mazandaran and Golestan provinces. Arch. Iranian Med. 4 (3): 107-114.

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Bibliografia

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