Effect of haloxyfob and cerulenin on de novo biosynthesis of lipids in roots of wheat and maize
Treść / Zawartość
The study examines the effects of haloxyfop (herbicide) and cerulenin (antibiotic) on de novo biosynthesis of fatty acids and complex lipids in roots of two sensitive species: wheat and maize. Seedlings were grown in hydroponic cultures with addition of [1-14C]acetate (control) and [1-14C]acetate together with one of the tested substances. Neither haloxyfop nor cerulenin prevented the uptake of [1-14C]acetate by the roots of tested species. In contrast, a strong inhibition of de novo biosynthesis of fatty acids was observed after a 4-h treatment. This phenomenon, however, tended to disappear with treatment time. After a 24-h incubation, the amount of radioactivity in de novo biosynthesized fatty acids in 1-cm-long root tips was up to three times higher than in the untreated control. In the "rest of roots", restoration of fatty acid biosynthesis capacity was less pronounced. Besides the effect on fatty acid biosynthesis, both tested inhibitors strongly suppressed the de novo biosynthesis of non-fatty acid-containing lipids. Analyses of radioactivity in individual lipid classes showed that after a 4-h treatment with haloxyfop or cerulenin the biosynthesis of most of the lipid classes was inhibited, although not to the same extent. After a 24-h treatment, an inhibition of de novo biosynthesis of some of the lipids was still observable, whereas the biosynthesis of others, especially phosphatidylethanolamine and phosphatidic acid, was strongly up-regulated. Contrary to the mainstream view that inhibition of fatty acid biosynthesis is the cause of haloxyfop and cerulenin phytotoxicity, the obtained results suggest multidirectional effects of both inhibitors.
- Institute of Biology, University of Natural Sciences and Humanities, Siedlce, Poland
- Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Słupsk, Poland
- Intercollegiate Faculty of Biotechnology of the University of Gdańsk and the Medical University of Gdańsk, Gdańsk, Poland
- Banaś A, Johansson I, Stenlid G, Stymne S (1990) The effect of haloxyfop-ethoxyethyl on lipid metabolism in oat and wheat shoots. Swedish J Agric Res 20: 97-104.
- Banaś A, Johansson I, Stenlid G, Stymne S (1993a) Free radical scavengers and inhibitors of lipoxygenases as antagonists against the herbicides haloxyfop and alloxydim. Swedish J Agric Res 23: 67-75.
- Banaś A, Johansson I, Stenlid G, Stymne S (1993b) The effect of haloxyfop and alloxydim on growth and fatty acid composition of wheat roots. Swedish J Agric Res 23: 55-65.
- Banaś A, Johansson I, Stenlid G, Stymne S (1993c) Investigation of the mode of action of the herbicide haloxyfop. Zesz Nauk Wyższ Szk Roln P Siedlce Ser Nauki Przyrodnicze 34: 1-19.
- Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917.
- Cobb A (1992) Herbicides and Plant Physiology. pp 107-125. Chapman and Hall, London.
- Christie WW (2012) Cardiolipin (diphosphatidylglycerol) - structure, occurrence, biology and analysis. http://lipidlibrary.aocs.org/lipids/
- De Prado JL, De Prado RH, Shimabukuro RH (1999) The effect of diclofop on membrane potential, ethylen induction, and herbicide phytotoxicity in resistant and susceptible biotypes of grasses. Pestic Biochem Physiol 63: 1-14.
- Feld A, Kobek K, Lichtenthaler HK (1989) Inhibition of fatty acid biosynthesis in isolated chloroplasts by the antibiotics cerulenin and thiolactomycin. Brighton Crop protection Conf - Weeds 1989, pp 479-486.
- Harwood JL (1999) Graminicides which inhibit lipid synthesis. Pesticide Outlook, pp 154-158.
- Heap IM, Morrison IN (1996) Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in green foxtail (Setaria viridis). Weed Sci 44: 25-30.
- Herbert D, Walker KA, Price LJ, Cole DJ, Pallett KE, Ridley SM, Harwood JL (1997) Acetyl-CoA carboxylase - a graminicide target site. Pestic Sci 50: 67-71.
- Hoppe HH (1985) Differential effect of diclofop-methyl on fatty acid biosynthesis in leaves of sensitive and tolerant plant species. Pesticide Biochem Physiol 23: 297-302.
- Jeong NY, Yoo YH (2012) Cerulenin-induced apoptosis is mediated by disrupting the interaction between AIF and hexokinase II. Int J Oncol 40: 1949-1956.
- Kim M, Lim JH, Ahn ChS, Park K, Kim GT, Kim WT, Pai HS (2006) Mitochondria-associated hexokinases play a role in the control of programmed cell death in Nicotiana benthamiana. The Plant Cell 18: 2341-2355.
- Lichtenthaler H (1990) Mode of action of herbicides affecting acetyl-CoA carboxylase and fatty acid biosynthesis. Zeitschr f Naturforsch 45c: 521-528.
- Luo XY, Sunohara Y, Matsumoto H (2004) Fluazifop-butyl causes membrane peroxidation in the herbicide-susceptible broad leaf weed bristly starbur (Acanthospermum hispidum). Pestic Biochem Physiol 78: 93-102.
- Nestler HJ (1982) Phenoxy-phenoxypropionic acid derivatives and related compounds. In Chemie der pflanzenscchutz und schädling-sbekämpfungsmittel Wegler R, ed, pp 1-25. Springer-Verlag, Berlin.
- Nilsson G (1977) Effects of glyphosate on the amino acid content in spring wheat plants. Swedish J Agric Res 7: 135-157.
- Packter NM, Stumpf PK (1975) Fat metabolism in higher plants. The effect of cerulenin on the synthesis of medium- and lon-chin acids in leaf tissue. Arch Biochem Biophys 167: 655-667.
- Palta JP, Whitaker BD, Weiss LS (1993) Plasma membrane lipids associated with genetic variability in freezing tolerance and cold acclimation of Solanum species. Plant Physiol 103: 793-803.
- Price LJ, Herbert D, Cole DJ, Harwood J (2003) Use of plant cell cultures to study graminicide effects on lipid metabolism. Phytochemistry 63: 533-541.
- Rendina AR, Felts JM, Beaudoin JD, Craig-Kennard AC, Look LL, Paraskos SL, Hagenah JA (1988) Kinetic characterization, stereoselectivity, and species selectivity of the inhibition of plant acetyl-CoA carboxylase by aryloxyphenoxypropionic acid grass herbicides. Arch Biochem Biophys 265: 219-225.
- Roughan PG, Slack CR (1982) Cellular organisation of glycerolipid metabolism. Annu Rev Plant Physiol 33: 97-132.
- Shimabukuro RH, Davis DG, Hoffer BL (2001) The effect of diclofop-methyl and its antagonist, vitamin E, on membrane lipids in oat (Avena sativa L.) and leafy spurge (Euphorbia esula L.). Pestic Biochem Physiol 69: 13-26.
- Shimabukuro RH, Hoffer BL (1996) Induction of ethylene as an indicator of senescence in the mode of action of diclofop-methyl. Pestic Biochem Physiol 54: 146-158.
- Shukla A, Dupont S, Devine MD (1997) Resistance to ACCase-inhibitor herbicides in wild oat: Evidence for target site-based resistance in two biotypes from Canada. Pestic Biochem Physiol 57: 147-155.
- Steponkus PL, Lynch DV, Uemura M (1990) The influence of cold acclimation on lipid composition and cryobehaviour of plasma membrane of isolated rye protoplasts. Phil trans R Lond B 326: 572-583.
- Tardif FJ, Preston C, Holtum JA, Povles SB (1996) Resistance to acetyl-coenzyme A carboxylase- inhibiting herbicides endowed by a single major gene encoding a resistant target site in biotype of Lolium rigidum. Aust J Plant Physiol 23: 15-23.
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