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2013 | 31 | 2 |
Tytuł artykułu

Polymorphisms of Zygote arrest 1 gene and their effects on litter size in pigs

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Zygote arrest 1 (ZAR1) is oocyte-specific protein involved in the initiation of embryo development. The objectives of this study were to identify novel mutations in ZAR1 gene and investigate the association between its genetic variants and litter size in Chinese local and European pigs. A novel single nucleotide polymorphism (SNP) in exon 3 (g.2612T>C) and a 5 bp deletion/insertion in intron 3 (g.3838-3839insTGCAG) were found by sequencing and then their genotypes were identified in 218 sows of five breeds. The results of association analysis showed that the SNP g.2612T>C was significantly related to litter size in Durocs (P<0.05), the sows with genotype CC genotype gave more piglets than those of TT or/and TC genotypes. What’s more, the litter size in the Duroc breed was also significantly dependent (P<0.05) on polymorphism in intron3 (g.3838-3839 sTGCAG). Sows with NN genotype had more piglets per litter than those of MM or/and MN genotypes. A similar relation (though not significant) was observed in remaining breeds tested. It is concluded that ZAR1 gene might be a potential important candidate gene related to litter size in pigs.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
31
Numer
2
Opis fizyczny
p.139-146,fig.,ref.
Twórcy
autor
  • Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan 430064, P.R.China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
autor
  • Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
autor
  • Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, Huazhong Agricultural University, Hubei, Wuhan 430070, China
Bibliografia
  • BREVINI T.A., CILLO F., COLLEONI S., LAZZARI G., GALLI C., GANDOLFI F., 2004 – Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos. Molecular Reproduction and Development 69, 375-380.
  • LATHAM K.E., 1999 – Mechanisms and control of embryonic genome activation in mammalian embryos. International Review of Cytology 193, 71-124.
  • LATHAM K.E., SCHULTZ R.M., 2001 – Embryonic genome activation. Frontiers in Bioscience 6, 748-759.
  • 4. LEE H.H., LO W.J., CHOO K.B., 1992 – Mutational analysis by a combined application of the multiple restriction fragment-single strand conformation polymorphism and the direct linear amplification DNA sequencing protocols. Analytical Biochemistry 205, 289-293.
  • PENNETIER S., UZBEKOVA S., PERREAU C., PAPILLIER P., MERMILLOS P., DALBIESTRAN R. 2004 – Spatio-temporal expression of the germ cell marker genes MATER, ZAR1,GDF9, BMP15, and VASA in adult bovine tissues, oocytes and preimplantation embryos. Biology Reproduction 71, 1359-1366.
  • ROTHCHILD M.F., JACOBSON C., VASKE D., 1996 – The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proceedings of the National Academy of Sciences of the United States of America 93, 201-205.
  • SAMBROOK J., FRITSCH E.F., MANIATIS T., 1989 – Molecular Cloning a Laboratory Manual. Cold Spring Harbor Laboratory Press. NY.
  • TAUTZ D., 1988 – Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres. Nature 332, 281-284.
  • UZBEKOVA S., ROY-SABAU M., DALBIES-TRAN R., PERREAU C., PAPILLIER P., MOMPART F., THELIE A., PENNETIER S., COGNIE J., CADORET V., ROYERE D., MONGET P., MERMILLOD P., 2006 – Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells. Reproductive Biology and Endocrinology 21, 4-12.
  • WU X., VIVEIROS M.M., EPPIG J.J., BAI Y., FITZPATRICK S.L., MATZUK M.M., 2003a –Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition.Nature Genetics 33, 187-191.
  • WU X., WANG P., BROWN C.A., ZILINSKI C.A., MATZUK M.M. 2003b – Zygote arrest 1 (Zar1) is an evolutionarily conserved gene expressed in vertebrate ovaries. Biology Reproduction 69, 861-867.
  • YUAN J.F., JAFER O., AFFARA N.A., GONG Y.Z., YANG L..G., LIU J., MOAEEN-UD-DINAL M., LI W.M., ZHANG S.J., 2007a – Association of four new single-nucleotide polymorphisms in follicle-stimulating hormone receptor and zona pellucida glycoprotein with reproductive traits in pigs. Animal 1, 1249-1253.
  • YUAN J.F., MOAEEN-UD-DINAL M., GONG Y.Z., PENG X.L., YANG L.G., PENG Y.P., LIU J., HU B., AFFARA N.A., JAFER O., ZHANG S.J., 2007b – Identification of Mutations of Zona Pellucida Glycoprotein (ZP3) and its Association with Pig Reproductive Traits. Journal of Animal Breeding and Genetics 124, 144-149.
  • ZHAO C.L.,JIAO L.H., LI X.M., CHEN X., SUN H.J., HAO Y.H., WANG L., 2006 – Maternal factors affecting development of early mouse embryos. Hereditas (Beijing) 28, 601-605.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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