Effects of body temperature and hyperferremia on glutathione and vitamine E levels in the brain of asphyxiated neonatal rat

• Autorzy: Rogalska J. , Kletkiewicz H. , Nowakowska A. , Wozniak A. , Mila-Kierzenkowska C.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: In asphyxiated newborns iron, deposited in the brain, contributes to formation of reactive oxygen species. METHODS: In our study we found an increased iron accumulation in neurons of rats exposed to neonatal anoxia under hyperthermic conditions which was prevented with deferoxamine (DFO) injection. Key factors in the cellular protection against oxidative stress are glutathione and α-tocopherol. Therefore, we decided to study the influence of body temperature and chelation of iron with deferoxamine on antioxidant status in the brain of rats exposed to critical anoxia. RESULTS: Two-day old newborn rats were exposed to anoxia in 100% nitrogen atmosphere. Rectal temperature was kept at 31°C (hypothermia), 33°C (physiological temperature in rat neonates), or at 37°C (hyperthermia) or at 39°C (extreme hyperthermia). Control rats were exposed to atmospheric air in respective thermal conditions. Half of the hyperthermic rats (39°C) exposed to anoxia were injected with DFO immediately after anoxia and 24 hours later. Cerebral concentrations of lipid peroxidation products (malondialdehyde – MDA) and the level of glutathione and α-tocopherol were determined post mortem: (1) immediately, (2) 72 hours, (3) 1 week after anoxia and (4) 2 weeks after anoxia. We observed that there were no postanoxic changes in concentration of MDA and in the level of glutathione and α-tocopherol in newborn rats kept at their physiological body temperature of 33°C. In contrast, simulated perinatal anoxia at body temperature elevated to 37°C and 39°C as well as hypothermic conditions (31°C) intensified post-anoxic oxidative stress and depleted the antioxidant pool. The decrease of antioxidants under hyperthermic conditions was prevented with postanoxic DFO injection. CONCLUSION: The data suggest that both elevated body temperature as well as cooling below the level of physiological body temperature of newborn rats may extend the perinatal anoxia-induced brain lesions. Research was supported by NCN under grant 2011/03/B/ NZ7/00682.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S50-S51

Twórcy

autor

Rogalska J.

• Department of Animal Physiology, Faculty of Biology and Environment Protection, N. Copernicus University, Torun, Poland

autor

Kletkiewicz H.

• Department of Animal Physiology, Faculty of Biology and Environment Protection, N. Copernicus University, Torun, Poland

autor

Nowakowska A.

• Department of Animal Physiology, Faculty of Biology and Environment Protection, N. Copernicus University, Torun, Poland

autor

Wozniak A.

• Department of Animal Physiology, Faculty of Biology and Environment Protection, N. Copernicus University, Torun, Poland

autor

Mila-Kierzenkowska C.

• Chair of Medical Biology, Collegium Medicum in Bydgoszcz, Toruń, Poland