PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 02 |
Tytuł artykułu

Cloning and expression of genes related to the sucrosemetabolizing enzymes and carbohydrate changes in peach

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
To shed light on the relationship between sucrose metabolism and expression of genes related to sucrose-metabolizing enzymes, six genes encoding sucrose-metabolizing enzymes were isolated, and the levels of four main carbohydrates and related enzyme activities as well as the expression of these six genes were determined in fruits, leaves and phloem-enriched fraction throughout peach fruit development. Sucrose content in mature fruit ranked first followed by glucose, fructose and sorbitol in that order, while sorbitol was the highest and sucrose lowest in phloem-enriched fraction and leaves. Glucose and fructose had similar change patterns throughout fruit development. Cloning results reveal that the nucleotide sequences of the six genes have high similarity to corresponding genes isolated from other plants. In addition, the expression of these genes and the levels of related enzyme activities varied with tissue and stage of fruit development, suggesting a complexity in relationships between carbohydrates, enzymes activities and related gene expression. Sucrose phosphate synthase maybe a key enzyme involved in sucrose synthesis while sucrose synthase may mainly be responsible for sucrose synthesis in peach fruits at later stages of development. Further studies are needed to genetically and physiologically characterize these genes and enzymes in peach and to gain a better understanding of their functions and relationship with carbohydrate metabolism.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
35
Numer
02
Opis fizyczny
p.589-602,fig.,ref.
Twórcy
autor
  • Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
autor
  • Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
autor
  • College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Weigang, People’s Republic of China
autor
  • College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Weigang, People’s Republic of China
autor
  • Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
autor
  • College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Weigang, People’s Republic of China
autor
  • Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
Bibliografia
  • Azcon-Bieto J (1983) Inhibition of photosynthesis by carbohydrates in wheat leaves. Plant Physiol 73:681–686
  • Baroja-Fernandez E, Munoz FJ, Saikusa T, Rodriguez-Lopez M, Akazawa T, Pozueta-Romero J (2003) Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants. Plant Cell Physiol 44:500–509
  • Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622
  • Chen CB, Zhu SH, Zhou J (2009) Improved CTAB method for total
  • RNA extraction of mature Feicheng peach fruit. Shandong Agri Sci 5:102–104
  • Cheng JS, Fan PG, Liang ZC, Wang YQ, Niu N, Li WD, Li SH (2009) Accumulation of end products in source leaves affects photosynthetic rate in peach via alteration of stomatal conductance and photosynthetic efficiency. J Amer Soc Hort Sci 134:667–676
  • Dali N, Michaud D, Yelle S (1992) Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. Plant Physiol 99:434–438
  • Dantas BF, Ribeiro LDS, Silva APD, Luz SRDS (2005) Foliar carbohydrates content and invertase activity in vines at Sao Francisco River Valley-Brazil. Rev Bras Frutic 27:198–202
  • Dunford S (1998) Translocation in the phloem. In: Taiz L, Zeigers E (eds) Plant physiology. Sinauer Associates, Sunderland ElSayed AI, Ramadan MF, Komor E (2010) Expression of sucrose transporter (ShSUT1) in a Hawaiian sugarcane cultivar infected with Sugarcane yellow leaf virus (SCYLV). Physiol Mol Plant Path 75:56–63
  • Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L, Cosson P, Pronier V, Monet R, Dirlewanger E (2002) Candidate genes and QTLs for sugar and organic acid content in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 105:145–159
  • Guo XF, Li SH, Liu GJ, Fu ZF, Li ST (2004) Seasonal changes in carbohydrate content and related enzyme activity in fruit and leaves of ‘Yanfengyihao’ peach variety. J Fru Sci 21:196–200
  • Heim U, Weber H, Wobus U (1996) Cloning and characterization of full-length cDNA encoding sucrose phosphate synthase from faba bean. Gene 178:201–203
  • Hubbard NL, Huber SC, Pharr DM (1989) Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melo L.) fruits. Plant Physiol 91:1527–1534
  • Hubbard NL, Phar DM, Huber SC (1990) Role of sucrose phosphate synthase in sucrose biosynthesis in ripening bananas and its relationship to the respiratory climacteric. Plant Physiol 94:201–208
  • Huber SC, Huber JL (1996) Role and regulation of sucrose-phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47:431–444
  • Ji XM, Van den Ende W, Van Laere A, Cheng SH, Bennett J (2005) Structure, evolution, and expression of the two invertase gene families of rice. J Mol Evol 60:615–634
  • Koch KE (1996) Carbohydrated-modulated gene expression in plants. Annu Rev Plant Physiol Plant Mol Biol 47:509–540
  • Krapp A, Stitt M (1995) An evaluation of direct and indirect mechanisms for the ‘‘sink-regulation’’ of photosynthesis in spinach: changes in gas exchanges, carbohydrates, metabolites, enzyme activities and steady-state transcript levels after coldgirdling source leaves. Planta 195:313–323
  • Kuhn C, Grof CPL (2010) Sucrose transporters of higher plants. Curr Opin Plant Biol 13:288–298
  • Liesche J, Kru¨gel U, He HX, Chincinska I, Hackel A, Ku¨hn C (2011) Sucrose transporter regulation at the transcriptional, posttranscriptional and post-translational level. J Plant Physiol 168:1426–1433
  • Lingle SE, Dunlap JR (1987) Sucrose metabolism in netted muskmelon fruit during development. Plant Physiol 84:386–389
  • Lingle SE, Dyer JM (2001) Cloning and expression of sucrose synthase-1 cDNA from sugarcane. Plant Physiol 158:129–131
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods 25:402–408
  • Lo Bianco R, Rieger M (2002) Partitioning of sorbitol and sucrose catabolism within peach fruit. J Am Soc Hortic Sci 127:115–121
  • Lombardo VA, Osorio S, Borsani J, Lauxmann MA, Bustamante CA, Budde CO, Andreo CS, Lara MV, Fernie AR, Drincovich MF (2011) Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage. Plant Physiol 157:1696–1710
  • Miron D, Schaffer AA (1991) Sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of Lycopersicon esculentum Mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiol 95:623–627
  • Moing A (2000) Sugar alcohols as carbohydrate reserves in some higher plants. In: Gupta AK, Kaur N (eds) Carbohydrate reserves in plants-synthesis and regulation. Dev Crop Sci 26:337–358
  • Moing A, Carbonne F, Rashad MH, Gaudille‘re JP (1992) Carbon fluxes in mature peach leaves. Plant Physiol 100:1878–1884
  • Moing A, Svanella L, Rolin D (1998) Compositional changes during the fruit development of two peach cultivars differing in juice acidity. J Amer Soc Hort Sci 123:770–775
  • Morandi B, Corelli Grappadelli L, Rieger M, Lo Bianco R (2008) Carbohydrate availability affects growth and metabolism in peach fruit. Physiol Plant 133:229–241
  • Moriguchi T, Yamaki S (1988) Purification and characterization of sucrose synthase from peach (Prunus persica) fruit. Plant Cell Physiol 29:1361–1366
  • Moscatelloa S, Famianib F, Proietti S, Farinelli D, Battistelli A (2011) Sucrose synthase dominates carbohydrate metabolism and relative growth rate in growing kiwifruit (Actinidia deliciosa, cv Hayward). Sci Hort 128:197–205
  • Nii N (1997) Changes of starch and sorbitol in leaves before and after removal of fruits from peach trees. Ann Bot 79:139–144
  • Niu J, Zhao JB, Wu BH, Li SH, Liu GJ, Jiang Q (2006) Sugar and acid contents in peach and nectarine derived from different countries and species. Acta Hort Sin 33:6–11
  • Nonis A, Ruperti B, Falchi R, Casatta E, Enferadi ST, Vizzotto G (2007) Differential expression and regulation of a neutral invertase encoding gene from peach (Prunus persica): evidence for a role in fruit development. Physiol Plant 129:436–446
  • Paul MJ, Foyer CH (2001) Sink regulation of photosynthesis. J Exp Bot 52:1383–1400
  • Robertson JA, Horvat RJ, Lyon BG, Meredith FI, Senter SD, Okie WR (1990) Comparison of quality characteristics of selected yellow- and white-fleshed peach cultivars. J Food Sci 55:1308–1311
  • Roitsch T, Gonza0lez MC (2004) Function and regulation of plant invertases: sweet sensations. Trends Plant Sci 9:606–613
  • Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell 14:185–205
  • Shen ZJ, Ma RJ, Yu ML, Cai ZX, Song HF, Li X (2007) Regularity analysis of main sugar and acid in fruit development of peach. Acta Agri Bor Occid Sin 22:130–135
  • Tian HM, Kong QG, Feng YQ, Yu XY (2009) Cloning and characterization of a soluble acid invertase-encoding gene from muskmelon. Mol Biol Rep 36:611–617
  • Tong ZG, Zhang Z, Wang FR (2007) Molecular cloning and characterization of a sucrose phosphate synthase gene from peach (Prunus persica). Submitted to the EMBL/GenBank/DDBJ databases
  • Tong ZG, Gao ZH, Wang F, Zhou J, Zhang Z (2009) Selection of reliable reference genes for gene expression studies in peach using real-time PCR. BMC Mol Biol 10:1–13
  • Verma AK, Upadhyay SK, Verma PC, Solomon S, Singh SB (2011) Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biol (Stuttg) 13:325–332
  • Vizzotto G, Pinton R, Varanini Z, Costa G (1996) Sucrose accumulation in developing peach fruit. Physiol Plant 96:225–230
  • Wan SB, Zhang B, Zhan JC, Chen JY, Yin JY (2009) In Silico cloning and sequence analysis of phospholipase Da gene from peach fruit. Agri Sci Chin 8:1293–1300
  • Wang YQ, Wu BH, Zhao JB, Jiang Q, Li SH (2008) Soluble sugar contents in fruits and leaves during fruit development and their relationship in peach cultivars of difference in fruit glucose/fructose. Sci Agri Sin 41:2063–2069
  • Wibbe ML, Blanke MM (1995) Effects of defruiting on source-sink relationship, carbon budget, leaf carbohydrate content and eater use efficiency of apple trees. Physiol Plant 94:529–533
  • Winter H, Huber SC (2000) Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Crit Rev Biochem Mol Biol 35:253–289
  • Wobus U, Weber H (1999) Sugars as signal molecules in plant seed development. Biol Chem 380:937–944
  • Wrolstad RE, Shallenberger RS (1981) Free sugars and sorbitol in fruits: a compilation from the literature. J Assoc Off Anal Chem 64:91–103
  • Zhang YP, Wang C, Yu HP, Cai BH, Fang JG (2010) Screening of RNA extraction methods for various grapevine organs and tissues. Acta Agri Bor Occid Sin 19:135–140
  • Zhang XM, Dou MA, Yao YL, Du LQ, Li JG, Sun GM (2011) Dynamic analysis of sugar metabolism in differ harvest seasons of pineapple (Ananas comosu L. (Merr.)). Afr J Biotechnol 10:2716–2723
  • Zhou R, Quebedeaux B (2003) Changes in photosynthesis and carbohydrate metabolism in mature apple leaves in response to whole plant source-sink manipulation. J Amer Soc Hort Sci 128:113–119
Uwagi
rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-4c1da64e-1b62-4cc8-9280-a5931a3d3c79
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.