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2011 | 29 | 3 |

Tytuł artykułu

Characterization and imprinting analysis of COPG2 and MEST genes in pigs

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Języki publikacji



MEST and COPG2 in human MEST imprinted cluster are two of good candidate genes responsible for primordial growth retardation including Silver–Russell syndrome. In order to increase understanding of these genes in pigs, their cDNAs are characterized in this report. By real-time quantitative RT-PCR and polymorphism-based method, tissue and allelic expression of both genes were determined using F1 reciprocal Landrace × Rongchang pig crossbreds. The transcription levels of MEST differed between tissues and decreased as development proceeded. The gene was imprinted and paternally expressed in heart, stomach, skeletal muscle, kidney, lung, bladder,tongue and fat, while biallelic expression was detected in liver, small intestine and spleen of onemonth-old pigs. The porcine COPG2 was differentially expressed between neonatal tissues and showed biallelic expression in postnatal tissues. Furthermore, the transcript of COPG2 in bladder and small intestine increased with age. It is concluded that tissue expression of porcine MEST is similar to, while COPG2 differs from other mammalian homologues. In addition, porcine MEST has development-specific imprinting, but imprinting of COPG2 in mammals is controversial.








Opis fizyczny



  • School of Life Sciences, Southwest University, Chongqing 400715, China


  • ANDRADE A.C., LUI J.C., NILSSON O., 2010 – Temporal and spatial expression of a growthregulated network of imprinted genes in growth plate. Pediatric Nephrology 25, 617-623.
  • BLAGITKO N., SCHULZ U., SCHINZEL A.A., ROPERS H.H., KALSCHEUER V.M., 1999 – γ2-COP, a novel imprinted gene on chromosome 7q32, defines a new imprinting cluster in the human genome. Human Molecular Genetics 8(13), 2387-2396.
  • CHARALAMBOUS M., DAROCHA S.T., FERGUSON-SMITH A.C., 2007 – Genomic imprinting,growth control and the allocation of nutritional resources: consequences for postnatal life. Current Opinion in Endocrinology, Diabetes and Obesity 14(1), 3-12.
  • DE KONING D.J., RATTINK A.P., HARLIZIUS B., VANARENDONK J.A., BRASCAMP E.W.,GROENEN M.A., 2000 – Genome-wide scan for body composition in pigs reveals important role of imprinting. Proceedings of the National Academy of Sciences of the USA 97, 7947-7950.
  • EDWARDS D.B, ERNST C.W., RANEY N.E., DOUMIT M.E., HOGE M.D., BATES R.O., 2008 – Quantitative trait locus mapping in an F2 Duroc × Pietrain resource population: II. Carcass and meat quality traits. Journal of Animal Science 86, 254-266.
  • FARES F., DAVID M., LERNER A., DIUKMAN R., LERER I., ABELIOVICH D., RIVLIN J., 2006 – Paternal isodisomy of chromosome 7 with cystic fibrosis and overgrowth. Amercian Journal of Medical Genetics, Part A 140(16), 1785-1788.
  • FEIL R., KHOSLA S., CAPPAI P., LOI P., 1998 – Genomic imprinting in ruminants: Allele-specific gene expression in parthenogenetic sheep. Mammalian Genome 9, 831-834.
  • HAHN Y., YANG S.K., CHUNG J.H., 2005 – Structure and expression of the zebrafish mest gene,an ortholog of mammalian imprinted gene PEG1/MEST. Biochimica et Biophysica Acta 1731, 125-132.
  • HANNULA. K., LIPSANEN-NYMAN M., KONTIOKARI T., KERE, J., 2001 – A narrow segment of maternal uniparental disomy of chromosome 7q31-qter in Silver-Russell syndrome delimits a candidate gene region. Amercian Journal of Human Genetics 68, 247-253.
  • HAYASHIDA S., YAMASAKI K., ASADA Y., SOEDA E., NIIKAWA N., KISHINO T., 2000 –Construction of physical and transcript map in the putative 7q32 imprinted region flanking MEST/PEG1. Genomics 66, 221-225.
  • KANEKO-ISHINO T., KUROIWA Y., MIYOSHI N., KOHDA T., SUZUKI R., YOKOYAMA M.,VIVILLE S., BARTON S.C., ISHINO F., SURANI, M.A., 1995 – Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization. Nature Genetics 11, 52-59.
  • KHATIB H., 2005 – Characterization and analysis of the imprinting status of the ovine SDHD and COPG2 genes. Animal Genetics 36, 160-190.
  • KOBAYASHI S., KOHDA T., MIYOSHI N., KUROIWA Y., AISAKA K., TSUTSUMI O., KANEKOISHINO T., ISHINO1 F., 1997 – Human PEG1/MEST, an imprinted gene on chromosome 7. Human Molecular Genetics 6(5), 781-786.
  • LEE Y.J., PARK C.W., HAHN Y., PARK J., LEE J., YUN J.H., HYUN B., CHUNG J.H., 2000 – Mit1/Lb9 and Copg2, new members of mouse imprinted genes closely linked to Peg1/Mest. FEBS Letters 472, 230-234.
  • LEFEBVRE L., VIVILLE S., BARTON S.C., ISHINO F., KEVERNE E.B., SURANI M.A., 1998 – Abnormal maternal behaviour and growth retardation associated with loss of the imprinting gene Mest. Nature Genetics 20, 163-169.
  • LIVAK K.J., SCHMITTGEN T.D., 2001 – Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4), 402-408.
  • LUEDI P.P., HARTEMINK A.J., JIRTLE R.L., 2005 – Genome-wide prediction of imprinted murine genes. Genome Research 15, 875-884.
  • LUI J.C., FINKIELSTAIN G.P., BARNES K.M., BARON, J., 2008 – An imprinted gene network that controls mammalian somatic growth is down-regulated during postnatal growth deceleration in multiple organs. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 295, R189-R196.
  • MILAN D., HAWKEN R., CABAU C., LEROUX S., GENET C., LAHBIB Y., TOSSER G., ROBIC A., HATEY F., ALEXANDER L., BEATTIE C., SCHOOK L., YERLE M., GELLIN J., 2000 –IMpRH server: an RH mapping server available on the web. Bioinformatics 16, 558-559.
  • NIKAIDO I., SAITO C., MIZUNO Y., MEGURO M., BONO H., KADOMURA M., KONO T., MORRIS G.A., LYONS P.A., OSHIMURA M., RIKEN GER GROUP, GSL MEMBERS,HAYASHIZAKI Y., OKAZAKI Y., 2003 – Discovery of imprinted transcripts in the mouse transcriptome using large-scale expression profiling. Genome Research 13, 1402-1409.
  • RUDDOCK N.T., WILSON K.J., COONEY M.A., KORFIATIS N.A., TECIRLIOGLU R.T.,FRENCH A.J., 2004 – Analysis of imprinted messenger RNA expression during bovine preimplantation development. Biology of Reproduction 70, 1131-1135.
  • SUZUKI S., RENFREE M.B., PASK A.J., SHAW G., KOBAYASHI S., KOHDA T., KANEKOISHINO T., ISHINO F., 2005 – Genomic imprinting of IGF2, p57KIP2 and PEG1/MEST in a marsupial, the tammar wallaby. Mechanisms of Development 122, 213-222.
  • XU C.C., SU L.J., ZHOU Q.Y., LI C.C. , ZHAO S.H., 2007 – Imprinting analysis of the porcine MEST gene in 75 and 90 day placentas and prenatal tissues. Acta Biochimica et Biophysica Sinica 39(8), 633-639.
  • YERLE M., PINTON P., ROBIC A., ALFONSO A., PALVADEAU Y., DELCROS C., HAWKEN R., ALEXANDER L., BEATTIE C., SCHOOK L., MILAN D., GELLIN J., 1998 – Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogeneics and Cell Genetics 82, 182-188.

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