Characterization and imprinting analysis of COPG2 and MEST genes in pigs

• Autorzy: Jiang C. , Li S. , Shi P. , Dong R. , Tian J. , Deng C.
• Języki publikacji: EN

Abstrakty

EN

MEST and COPG2 in human MEST imprinted cluster are two of good candidate genes responsible for primordial growth retardation including Silver–Russell syndrome. In order to increase understanding of these genes in pigs, their cDNAs are characterized in this report. By real-time quantitative RT-PCR and polymorphism-based method, tissue and allelic expression of both genes were determined using F1 reciprocal Landrace × Rongchang pig crossbreds. The transcription levels of MEST differed between tissues and decreased as development proceeded. The gene was imprinted and paternally expressed in heart, stomach, skeletal muscle, kidney, lung, bladder,tongue and fat, while biallelic expression was detected in liver, small intestine and spleen of onemonth-old pigs. The porcine COPG2 was differentially expressed between neonatal tissues and showed biallelic expression in postnatal tissues. Furthermore, the transcript of COPG2 in bladder and small intestine increased with age. It is concluded that tissue expression of porcine MEST is similar to, while COPG2 differs from other mammalian homologues. In addition, porcine MEST has development-specific imprinting, but imprinting of COPG2 in mammals is controversial.

Słowa kluczowe

EN

imprinting analysis; COPG2 gene; MEST gene; pig; tissue expression; gene expression

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 3
• Opis fizyczny: p.247-256,fig.,ref.

Twórcy

autor

Jiang C.

• School of Life Sciences, Southwest University, Chongqing 400715, China

autor

Li S.

autor

Shi P.

autor

Dong R.

autor

Tian J.

autor

Deng C.

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