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2012 | 17 | 2 |

Tytuł artykułu

Autophagy favors Brucella melitensis survival in infected macrophages

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.

Wydawca

-

Rocznik

Tom

17

Numer

2

Opis fizyczny

p.249-257,fig.,ref.

Twórcy

autor
  • College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
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Bibliografia

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  • 6. Romano, P.S., Gutierrez, M.G., Beron, W., Rabinovitch, M. and Colombo, M.I. The autophagic pathway is actively modulated by phase II Coxiella burnetii to efficiently replicate in the host cell. Cell. Microbiol. 9 (2007) 891-897.
  • 7. Gutierrez, M.G., Master, S.S., Singh, S.B., Taylor, G.A., Colombo, M.I. and Deretic, V. Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages. Cell 119 (2004) 753-765.
  • 8. Py, B.F., Lipinski, M.M. and Yuan, J. Autophagy limits Listeria monocytogenes intracellular growth in the early phase of primary infection. Autophagy 3 (2007) 117-126.
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  • 10. Smith, R., Adams, L.G., Sowa, B.A. and Ficht, T.A. Induction of lymphocyte responsiveness by the outer membrane-peptidoglycan complex of rough strains of Brucella abortus. Vet. Immunol. Immunopathol. 26 (1990) 31-48.
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  • 12. Starr, T., Ng, T.W., Wehrly, T.D., Knodler, L.A. and Celli, J. Brucella intracellular replication requires trafficking through the late endosomal/lysosomal compartment. Traffic 9 (2008) 678-694.
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  • 14. Pizarro-Cerda, J., Meresse, S., Parton, R.G., van der, G.G., Sola-Landa, A., Lopez-Goni, I., Moreno, E. and Orvel, J.P. Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes. Infect. Immun. 66 (1998) 5711-5724.
  • 15. Pizarro-Cerda, J. and Cossart, P. Bacterial adhesion and entry into host cells. Cell. 124 (2006) 715-727.
  • 16. Li, M., Jiang, X., Liu, D., Na, Y., Gao, G.F. and Xi, Z. Autophagy protects LNCaP cells under androgen deprivation conditions. Autophagy 4 ( 2008) 54-60.
  • 17. Yuk, J.M., Shin, D.M., Lee, H.M., Yang, C.S. and Jin, H.S. Vitamin D3 induces autophagy in human monocytes/macrophages via cathelicidin. Cell Host Microbe 6 (2009) 231-243.
  • 18. Espert, L., Denizot, M., Grimaldi, M., Robert, H.V., Gay, B. and Varbanov, M. Autophagy is involved in T cell death after binding of HIV-1 envelope proteins to CXCR4. J. Clin. Invest. 116 (2006) 2161-2172.
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  • 21. Gutierrez, M.G., Master, S.S., Singh, S.B., Taylor, G.A., Colombo, M.I. and Deretic, V. Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages. Cell 119 (2004) 753-766.
  • 22. Ogawa, M., Yoshimori, T., Suzuki, T., Sagara, H., Mizushima, N. and Sasakawa, C. Escape of intracellular Shigella from autophagy. Science 307 (2005) 727-737.
  • 23. Horwitz, M.A. The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome–lysosome fusion in human monocytes. J. Exp. Med. 158 (1983) 2108-2126.
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  • 25. Zhu, C., Wang, X., Xu, F., Bahr, B.A., Shibata, M., Uchiyama, Y., Hagberg, H. and Blomgren, K. The influence of age on apoptotic and other mechanisms of cell death after cerebral hypoxia-ischemia. Cell Death Diff. 12 (2005) 162-176.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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