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2015 | 14 | 4 |
Tytuł artykułu

In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

Treść / Zawartość
Warianty tytułu
PL
Regeneracja in vitro Citrus limon L. Burm cv. ‘Primofiore’ z eksplantatów liściowych
Języki publikacji
EN
Abstrakty
EN
In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t
PL
Organogenezę in vitro badano u Citrus limon L. Burm cv. ‘Primofiore’, wykorzystując eksplantaty z liści. Celem badań była optymalizacja warunków do indukcji tkanki kalusowej oraz regeneracji roślin C. limon. Eksplantaty C. limon wykładano na 16 pożywek różnego rodzaju, które dodatkowo uzupełniano kombinacjami roślinnych regulatorów wzrostu, zarówno auksyn, jak i cytokinin, takimi jak 6-benzylaminopuryna (BAP), kwas naftalenooctowy (NAA), kwas 2,4-dichlorofenoksyoctowy (2,4-D) i kinetyna. Regenerację pędową uzyskano, gdy eksplantaty liściowe wykładano na pożywkę Murashige and Tuckera uzupełnioną 3,5 mg·L-1 BAP. Badania histologiczne pozwoliły na przedstawienie początkowej fazy rozwoju eksplantatów z liści w trakcie regeneracji in vitro C. limon.
Wydawca
-
Rocznik
Tom
14
Numer
4
Opis fizyczny
p.143-153,fig.,ref.
Twórcy
  • Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland
  • Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland
autor
  • Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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