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2019 | 68 | 1 |
Tytuł artykułu

Microbiota and chemical compounds in fermented Pinelliae Rhizoma (Banxiaqu) from different areas in the Sichuan Province, China

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This study focused on the microbiota and chemical compounds of the fermented Pinelliae Rhizoma produced in Longchang (LC), Zizhong (ZZ) and Xindu (XD), in Sichuan Province (China). High-throughput sequencing was used to analyze the microbiota. GC-MS and LC-MS were used to detect the compounds produced during the three different Pinelliae Rhizoma fermentation processes. The bacteria and fungi of the three fermented Pinelliae Rhizoma differed substantially, with the bacterial content mainly composed of the Bacillus genus, while the common fungi were only included in four OTUs, which belong to three species of Eurotiomycetes and Aspergillus cibarius. 51 volatile compounds were detected; they varied between LC, XD, and ZZ fermented Pinelliae Rhizoma. C10 and C15 terpenes were most frequently detected, and only curcumene and β-bisabolene were detected in the three fermented Pinelliae Rhizoma. 65 non-volatile compounds were detected by LC-MS, most were of C16, C18, C20, C21 and C22 structures. Cluster analysis showed more similarity between LC and XD fermented Pinelliae Rhizoma with regards to volatile compound content, but more similarity between the XD and ZZ fermented Pinelliae Rhizoma for non-volatiles. Moreover, no correlation between geographical distance and microflora or compounds of fermented Pinelliae Rhizoma was observed. These results showed that hundreds of compounds are produced by the natural mixed fermentation of Pinelliae Rhizoma, and may mostly relate to the microorganisms of five species.
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  • Affiliated Hospital of Northern Sichuan Medical College, Nanchong, Sichuan, China
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin, China
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin, China
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin, China
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin, China
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin, China
  • Boratyński F, Szczepańska E, Grudniewska A, Olejniczak T.Microbial kinetic resolution of aroma compounds using solid-state fermentation. Catalysts. 2018;8(1):28. doi:10.3390/catal8010028
  • Carnesecchi S, Bras-Gonçalves R, Bradaia A, Zeisel M, Gossé F, Poupon MF, Raul F. Geraniol, a component of plant essential oils, modulates DNA synthesis and potentiates 5-fluorouracil efficacy on human colon tumor xenografts. Cancer Lett. 2004;215(1):53–59.doi:10.1016/j.canlet.2004.06.019 Medline
  • Catalan RE, Martinez AM, Aragones MD.In vitro effect of ecdy-sterone on protein kinase activity. Comp Biochem Physiol B Comp Biochem. 1982;71(2):301–303. doi:10.1016/0305-0491(82)90259-0
  • Chen H. Anaerobic Solid-State Fermentation. In: Modern Solid State Fermentation. Dordrecht (Netherlands): Springer Nether-lands. 2013; p. 199–242.
  • Chen Y, Zhang YQ, Qian BC, Gong WG, Xu HJ, Zhang LX.[Pharmacological study of geraniol] (in Chinese). Chinese Tradi-tional Patent Medicine. 1980;01:26–30.China National Standard. GB/T 23535-2009 [Lipase preparation] (in Chinese).
  • China National Standard. GB/T 24401-2009 [α-Amylase prepara-tion] (in Chinese).
  • China Commercial Standard. SB/T 10317-1999 [Measurement of proteinase activity] (in Chinese).
  • Chinese Pharmacopoeia Commission. Chinese pharmacopoeia 2015 part 1. (in Chinese). Beijing (China): China Medical Science & Technology Press. 2015; p. 135.
  • Cianchetta S, Bregoli L, Galletti S. Microplate-based evaluation of the sugar yield from giant reed, giant miscanthus and switchgrass after mild chemical pre-treatments and hydrolysis with tailored tri choderma enzymatic blends. Appl Biochem Biotechnol. 2017;183(3):876–892. doi:10.1007/s12010-017-2470-z
  • de Sousa DP, Quintans Jr. L, de Almeida RN. Evolution of the anticonvulsant activity of α-terpineol. Pharm Biol. 2007;45(1):69–70.
  • Editorial Committee of Chinese Materia Medica. State Admini-stration of Traditional Chinese Medicine. Chinese Materia Medica (in Chinese). Shanghai (China): Shanghai Science and Technology Publishing House. 2005.
  • Fan W, Tang Y, Qu Y, Cao F, Huo G. Infant formula supplemented with low protein and high carbohydrate alters the intestinal micro-biota in neonatal SD rats. BMC Microbiol. 2014;14:279.
  • Gong DF, Wang FC, Ji DH, Zhang L. [Advances in studies on chemical constituents, pharmacological and toxicological activities of Pinellia ternate]. (in Chinese). J Yangtze Univ (Nat Sci Ed). 2015; 12(18):77–79.
  • Grzanna R, Lindmark L, Frondoza CG. Ginger – an herbal medi-cinal product with broad anti-inflammatory actions. J Med Food. 2005;8(2):125–132.
  • Guo JJ, Su MS, Wang LY, Ren JX, Yang M, Xie XM. [Identification of dominant microorganisms in the processing of Pinellia Quai-lata]. (in Chinese). China Journal of Chinese Materia Medica. 2016. 41(16): 3027–3031.
  • Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci. 2008;65(11):1631–1652.
  • He GZ, Zhang J, Xue XM, Wang J. [The effect of ginsenosides on immune function enhancement and regulation in patients with chronic cor pulmonale]. (in Chinese). J Jilin Univ (Med Ed). 1993;1:50–53.
  • Hu C, Nögel R, Hummelsberger J, Engelhardt U. Monographie 40: Pinelliae Rhizoma (Banxia). In: Hu C, Nögel R, Hummelsberger J, Engelhardt U, editors. Paozhi: Die Aufbereitung chinesischer Arznei mittel. Berlin, Heidelberg (Germany): Springer. 2018.
  • Huan M, Cui H, Teng Z, Zhang B, Wang J, Liu X.In vivo anti-tumor activity of a new doxorubicin conjugate via α-linolenic acid. J Agric Chem Soc Japan. 2012;76(8):1577–1579.
  • Kõljalg U, Nilsson RH, Abarenkov K, Tedersoo L, Taylor AF, Bahram M, Bates ST, Bruns TD, Bengtsson-Palme J, Calla-ghan TM, et al. Towards a unified paradigm for sequence-based identification of fungi. Mol Ecol. 2013;22(21):5271–5277. doi:10.1111/mec.12481
  • Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, et al. Predictive functional profiling of microbial com-mu nities using 16S rRNA marker gene sequences. Nat Biotechnol. 2013;31(9):814–821. doi:10.1038/nbt.2676
  • Lemfack MC, Gohlke BO, Toguem SMT, Preissner S, Piechulla B, Preissner R. mVOC 2.0: a database of microbial volatiles. Nucleic Acids Res. 2018;46(D1):D1261–D1265. doi:10.1093/nar/gkx1016
  • Lu DH. [Quality evaluation of Pinellia ternata in different regions and identification of counterfeit and related varieties]. (in Chinese). Chengdu (China): Chengdu University of Traditional Chinese Medicine. 2012.Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules, 2010;15(12):9252–9287.
  • Mukherjee PK, Chandra J, Retuerto M, Sikaroodi M, Brown RE,Jurevic R, Salata RA, Lederman MM, Gillevet PM, Ghan-noum MA. Oral mycobiome analysis of HIV-infected patients: iden-tification of Pichia as an antagonist of opportunistic fungi. PLoS Pathog. 2014;10(3):e1003996. doi:10.1371/journal.ppat.1003996
  • Omar R, Igoli JO, Zhang T, Gray AI, Ebiloma GU, Clements CJ, Fearnley J, Ebel RE, Paget T, Koning HP, et al. The chemical characterization of Nigerian propolis samples and their activity against Trypanosoma brucei. Sci Re. 2017;7(1):923. doi:10.1038/s41598-017-01038-2Peng DU. [Artificial alkaloids and chemical constituents of Gentiana crassicaulis Duthie ex Burk]. (in Chinese). J Anhui Agric Sci. 2016;44(26):91–94.
  • Qu HH, Qu BP, Liu SC, Qin GF, Kong H, Zhao Y. Mechanism of baicalin compatibility in Chinese medicine formula banxia xiexin decoction by pharmacokinetics and indirect competitive enzyme-linked immunosorbent assays in mice. Chin J Integr Med. 2016;2:1–5.
  • Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013;41(Database issue):D590–D596. doi:10.1093/nar/gks1219
  • Rédei GP. Kjeldahl Method. In: Rédei GP. Encyclopedia of Gene -tics, Genomics, Proteomics and Informatics. Dordrecht (Nether-lands): Springer Netherlands. 2008.Shen DR, Zhang LR, Ye LL. [Analysis of the efficacy of fermented Pinellia ternate]. (in Chinese). China Pharm. 2009;18(16):73–74.
  • Shirvani MA, Motahari-Tabari N, Alipour A. The effect of mefenamic acid and ginger on pain relief in primary dysmenorrhea: a randomized clinical trial. Arch Gynecol Obstet. 2015;291(6): 1277–1281.
  • Si J, Tong Z, Zeng Y, Jiang X, Liu R. Quality evaluation and utilization of germplasm resources of Magnolia officinalis. Journal of Chinese Medicinal Materials. 2002;25(2):79.
  • Try S, Voilley A, Chunhieng T, De-Coninck J, Waché Y. Aroma compounds production by solid state fermentation, importance of in situ gas-phase recovery systems. Appl Microbiol Biotechnol. 2018;102(17):7239–7255.
  • Wagner H, Bauer R, Melchart D, Xiao PG, Staudinger A.Rhizoma pinelliae – Banxia. In: Wagner H, Bauer R, Melchart D, Xiao PG, Staudinger A, editors. Chromatographic Fingerprint Analysis of Herbal Medicines. Vienna (Austria): Springer. 2011.
  • Wu X, Wang S, Lu J, Jing Y, Li M, Cao J, Bian B, Hu C. Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives. Chin Med. 2018;13:4.doi:10.1186/s13020-018-0163-3
  • Xiao Q, Yang WW, Zhang DW, Yi XX. [Advances in the study of influencing factors and pharmacological effects of total alkaloids in Pinellia ternata]. (in Chinese). Chinese Pharmaceuticals. 2016; 25(3):123–126.
  • Zhang FJ. [Preparation and bioactivity of total lactones from Betula pilosa]. (in Chinese). Doctoral dissertation. Shanghai (China): Second Military Medical University. 2009.
  • Zhao H, Yun J. Isolation, identification and fermentation conditions of highly acetoin-producing acetic acid bacterium from Liangzhou fumigated vinegar in China. Ann Microbiol. 2016;66(1): 279–288. doi:10.1007/s13213-015-1106-1
  • Zhao JL, Li Q, Ding YY, Gu XY, Feng WW, Wang W, Zhang M, Zhao T, Yang LQ, Wu XY. Sedative activity of the chemical constituents of Rhizoma pinelliae, praeparatum. Chem Nat Compd. 2018;54(1): 215–217.doi:10.1007/s10600-018-2304-4
  • Zhou Q, Wu H, Wang QR, Lu AJ. Anti-inflammatory effects of total alkaloids in Pinellia ternata]. (in Chinese). J Nanjing Univ Trad Chinese Med. 2006;22(2):86–88.
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