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2014 | 54 | 2 |
Tytuł artykułu

Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.
Wydawca
-
Rocznik
Tom
54
Numer
2
Opis fizyczny
p.199-203,fig.,ref.
Twórcy
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
autor
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
autor
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Bibliografia
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  • for classification and identification of mycoplasma-like organisms. Phytopathology 83 (8): 834–842.
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  • Martini M., Lee I.M., Bottner K.D., Zhao Y., Botti S., Bertaccini A., Harrison N.A., Carraro L., Marcone C., Khan A.J., Osler R. 2007. Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int. J. Syst. Evol. Microbiol. 57 (9): 2037–2051.
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  • Vali Sichani F., Bahar M., Zirak L. 2014. Characterization of phy-toplasmas related to aster yellows group infecting annual plants in Iran based on 16S rRNA and rp genes studies. J. Plant Prot. Res. 54 (1): 1–8.
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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