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2014 | 54 | 2 |
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Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ subgroup rpI-L in Iran

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In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.
Opis fizyczny
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
  • Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
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