Transcriptional pattern of TGF-beta1 inhibitory effect on mouse C2C12 myoblasts differentiation

• Autorzy: Wicik Z. , Sadkowski T. , Jank M. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to define the effect of TGF-β1 on C2C12 myoblasts myogenesis. TGF-β1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-β1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-β1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-β1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-β1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-β1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Bax, MdfI, Tef, Tubg1, Cxcl5, Rho, Calca and Lgals4.

Słowa kluczowe

EN

transforming growth factor-beta 1; myogenesis; microarray; DNA microarray; differentiation; myoblast; mouse; mice; muscle cell; myosin heavy chain

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2010
• Tom: 13
• Numer: 4
• Opis fizyczny: p.629-638,fig.,ref.

Twórcy

autor

Wicik Z.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Sadkowski T.

autor

Jank M.

autor

Motyl T.

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