The effect of culture medium formulation on Pinus nigra somatic embryogenesis

• Autorzy: Salaj T. , Fraterova L. , Carach M. , Salaj J.
• Języki publikacji: EN

Abstrakty

EN

The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.

Słowa kluczowe

EN

culture medium; formulation; Pinus nigra; European black pine; histology; conifer; plant growth regulator; somatic embryo; somatic embryogenesis

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2014
• Tom: 71
• Opis fizyczny: p.119-128,fig.,ref.

Twórcy

autor

Salaj T.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovakia

autor

Fraterova L.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovakia

autor

Carach M.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovakia

autor

Salaj J.

• Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra, Slovakia

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Identyfikatory

DOI

10.12657/denbio.071.012