Primordial germ cells ( PGCs) as a tool for creating transgenic chickens

• Autorzy: Chojnacka-Puchta L. , Kasperczyk K. , Plucienniczak G. , Sawicka D. , Bednarczyk M.
• Języki publikacji: EN

Abstrakty

EN

The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.

Słowa kluczowe

EN

primordial germ cell; transgenic animal; chicken; transgene expression; bioreactor; ovalbumin

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 1
• Opis fizyczny: p.181-188,fig.,ref.

Twórcy

autor

Chojnacka-Puchta L.

• Institute of Biotechnology and Antibiotics, Staroscinska 5, 02-516 Warsaw, Poland

autor

Kasperczyk K.

autor

Plucienniczak G.

autor

Sawicka D.

autor

Bednarczyk M.

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