In vitro propagation from axenic explants of Lilium oxypetalum (D.Don) Baker, an endemic bulbous plant of high altitude Himalaya

• Autorzy: Joshi S.K. , Dhar U.
• Języki publikacji: EN

Abstrakty

EN

In vitro propagation protocol for Lilium oxypetalum, a high altitude Himalayan endemic lily, has been developed. Effect of explant types (i.e., callus and in vitro bulblet scales) and sucrose concentration [0–6.0% (w/v)] on in vitro bulblet regeneration of L. oxypetalum was tested in previously optimized Murashige and Skoog basal medium supplemented with 2.0 µM 6-benzyladenine and 0.1 µM αnaphthaleneacetic acid. Callus explants produced significantly (P < 0.01) higher number of bulblets per explant than bulblet scale explants. Of the different concentrations of sucrose tested, 4.5% (w/v) sucrose showed significantly (P < 0.01) higher percentage regeneration (i.e., 70.8 ± 4.2 and 79.2 ± 4.2% regeneration on callus and bulblet scale explants, respectively), and produced higher number of bulblets per explant (i.e., 9.0 ± 0.4 and 5.4 ± 0.5 bulblets on callus and bulblet scale explants, respectively). Regenerated bulblets developed 2–3 leaves when subcultured for 4 weeks and were subsequently transferred ex vitro with a survival rate of 66.7% after 6 weeks. Leaves of the survived plantlets became dry after growing ex vitro for 10 weeks, amongst which 86.4% re-sprouted after remaining dormant for 5–6 weeks and produced 1.5 bulblets per explant. Findings of the present study hold promise for efficiently multiplying the target species in view of its potential economic and conservation significance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 4
• Opis fizyczny: p.833-838,fig.,ref.

Twórcy

autor

Joshi S.K.

• G.B. Plant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263643, Uttarakhand, India

autor

Dhar U.

• G.B. Plant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263643, Uttarakhand, India

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