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2009 | 69 | 4 |

Tytuł artykułu

Immunolocalization of STIM1 in the mouse brain

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Capacitative Calcium Entry (CCE) in neurons seems to depend, as in non-excitatory cells, on endoplasmic reticulum calcium sensors STIM1 or STIM2. We show localization of STIM1 in the mouse brain by immunohistochemistry with a specific antibody. STIM1 immunoreactivity has wide, but not uniform, distribution throughout the brain and is observed in neuropil and cells. The most intensive immunoreactivity is observed in Purkinje neurons of cerebellum. High/moderate levels of immunostaining are found in hippocampus, cerebral cortex and in cortico-medial amygdala, low in thalamus and basolateral amygdala. Co-staining with anti-NeuN antibody identify STIM1 immunopositive cells as neurons. Real time PCR demonstrates that Stim2 expression is 7-fold higher than that of Stim1 in hippocampus and 3-fold in other regions. Immunoblotting confirms that levels of STIMs vary in different brain regions. The data show that STIM1 and STIM2 are present in the brain, thus both can be involved in CCE, depending on neuronal type.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

69

Numer

4

Opis fizyczny

p.413–428,fig.,ref.

Twórcy

  • Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warsaw, Poland
  • Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warsaw, Poland
  • Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warsaw, Poland
autor
  • Department of Neurology, Heinrich Heine University, Düsseldorf, Germany
autor
  • Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Warsaw, Poland
  • Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

Bibliografia

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  • Berna-Erro A, Braun A, Kraft R, Kleinschnitz C, Schuhmann MK, Stegner D, Wultsch T, Eilers J, Meuth SG, Stoll G, Nieswandt B (2009) STIM2 regulates capacitive Ca2+ entry in neurons and plays a key role in hypoxic neuronal cell death. Sci Signal 2: ra67.
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  • Bouron A (2000) Activation of a capacitative Ca(2+) entry pathway by store depletion in cultured hippocampal neu­rones. FEBS Lett 470: 269-272.
  • Dziadek MA, Johnstone LS (2007) Biochemical properties and cellular localisation of STIM proteins. Cell Calcium 42: 123-132.
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  • Klejman ME, Gruszczynska-Biegala J, Skibinska-Kijek A, Wisniewska MB, Misztal K, Blazejczyk M, Bojarski L, Kuznicki J (2009) Expression of STIM1 in brain and puncta-like co-localization of STIM1 and ORAI1 upon depletion of Ca(2+) store in neurons. Neurochem Int 54: 49-55.
  • Liao Y, Erxleben C, Abramowitz J, Flockerzi V, Zhu MX, Armstrong DL, Birnbaumer L (2008) Functional interac­tions among Orai1, TRPCs, and STIM1 suggest a STIM- regulated heteromeric Orai/TRPC model for SOCE/Icrac channels. Proc Natl Acad Sci U S A 105: 2895-900.
  • Liou J, Kim ML, Heo WD, Jones JT, Myers JW, Ferrell JE Jr, Meyer T (2005) STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx. Curr Biol 15: 1235-1241.
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  • Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA (2001) Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J 357: 673-685.
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