Determination of sperm acrosin activity in the arctic fox (Alopex lagopus L.) – using method developed for human spermatozoa

• Autorzy: Stasiak K. , Janicki B. , Glogowski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to adapt a method to determine acrosin activity of human spermatozoa to arctic fox (Alopex lagopus L.) spermatozoa. We modified this method by reducing sperm count per sample from 1÷10 × 106 to 25÷200 × 103, incubation time from 180 minutes to 60 minutes, and Triton X-100 concentration in the reaction mixture from 0.01% to 0.005% per 100 cm3. It has also confirmed that arctic fox seminal plasma is rich in proteinases and their inhibitors. To completely abolish the inhibitory effect of seminal plasma on acrosin activity it is recommended to wash the spermatozoa four times. Benzamidine served an inhibitor of acrosin activity.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 4
• Opis fizyczny: p.799-800,ref,

Twórcy

autor

Stasiak K.

• University of Technology and Life Sciences in Bydgoszcz, Faculty of Animal Breeding and Biology, Department of Biology of Small Ruminants and Environmental Biochemistry, Mazowiecka 28, 85-084 Bydgoszcz, Poland

autor

Janicki B.

• University of Technology and Life Sciences in Bydgoszcz, Faculty of Animal Breeding and Biology, Department of Biology of Small Ruminants and Environmental Biochemistry, Mazowiecka 28, 85-084 Bydgoszcz, Poland

autor

Glogowski J.

• Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, University of Warmia and Mazury in Olsztyn, Faculty of Environmental Sciences and Fisheries, Department of Ichthyology, M. Oczapowskiego 5, 10-719 Olsztyn, Poland

Bibliografia

• Glogowski J, Demianowicz W, Piros B, Ciereszko A (1998) Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen. Theriogenology 50: 861-872.
• Glogowski J, Jankowski J, Faruga A, Ottobre JS, Ciereszko A (2001) Acrosin activity in turkey spermatozoa: assay by clinical method and effect of zinc and benzamidine on the activity. Theriogenology 56: 889-901.
• Kennedy WP, Kaminski JM, Van der Ven HH, Jeyendran RS, Reid DS, Blackwell J, Bielfeld P, Zaneveld LJD (1989) A simple, clinical assay to evaluate the acrosin activity of human spermatozoa. J Androl 10: 221-231.
• Mari SI, Rawe V, Biancotti JC, Charreau EH, Dain L, Vazquez-Levin MH (2003) Biochemical and molecular studies of the proacrosin/acrosin system in patients with unexplained infertility. Fertil Steril 79: 1676-1679.
• Stasiak K, Janicki B (2007) Activity of the trypsin inhibitor on the semen plasma of the polar fox Alopex lagopus L. during the reproductive season. Medycyna Wet 63: 990-993.

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