Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses

• Autorzy: Min D-H , Zhao Y. , Huo D.-Y. , Li L.-C. , Chen M. , Xu Z. S. , Ma Y.-Z.
• Języki publikacji: EN

Abstrakty

EN

Zinc finger protein is one of the proteins with finger-like domains. CCCH-type zinc finger proteins involved in plant stress tolerance are poorly understood. In this paper, a novel CCCH-type zinc finger protein gene TaZnFP was isolated during analysis of an abiotic stress suppression subtractive hybridization expression library from wheat (Triticum aestivum L.). The TaZnFP gene contains a 1,533 bp open reading frame with no intron and encodes a 55.2 kDa protein with 510 amino acids. Its isoelectric point (pI) is 8.94. The TaZnFP protein contains two typical CCCH-type zinc finger domains (CX9CX5CX3H and CX5CX4CX3H). Transient expression of a 35S:: TaZnFP-GFP fusion protein in Arabidopsis mesophyll protoplasts showed that TaZnFP was localized in the cytoplasm. Q-RT-PCR results showed that the TaZnFP transcript was induced by cold, salt (NaCl), drought, and ABA. Overexpression of TaZnFP enhanced drought and salt stress tolerance in transgenic Arabidopsis compared to the control. TaZnFP might be an important regulator responsive to abiotic stresses in plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 05
• Opis fizyczny: p.1597-1604,fig.,ref.

Twórcy

autor

Min D-H

• College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Regions Crop Adversity Biology, Yangling 712100, Shanxi, China

autor

Zhao Y.

• College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Regions Crop Adversity Biology, Yangling 712100, Shanxi, China

autor

Huo D.-Y.

• College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Regions Crop Adversity Biology, Yangling 712100, Shanxi, China

autor

Li L.-C.

• Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China

autor

Chen M.

• Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China

autor

Xu Z. S.

• Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China

autor

Ma Y.-Z.

• Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China

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