Population genetics of the narrow endemic Hlandnikia pastinacifolia Rchb. (Apiaceae) indicates survival in situ during the pleistocene

• Autorzy: Sajna N. , Kavar T. , Sustar-Vozlic J. , Kaligaric M.
• Języki publikacji: EN

Abstrakty

EN

Hladnikia pastinacifolia Rchb., a narrow endemic, has an extremely restricted distribution in Trnovski gozd (Slovenia), despite the presence of many sites with suitable habitats. We compared the morphological traits of plants from different populations and habitats. The overall pattern showed that the smallest plants, with low fruit number, are found on Èaven (locus classicus or type locality); the largest individuals, with high fruit number, grow in the Golobnica gorge. As judged by plant size and seed set, the optimal habitats are screes. We used RAPD markers to estimate genetic variation between and within populations, as well as between and within the northern and the southern parts of the distribution area. Hladnikia showed only a low level of RAPD variability. AMOVA partitioned the majority of genetic diversity within selected populations. The low genetic differentiation between populations and their genetic depauperation indicates survival in situ, since the Trnovski gozd plateau most likely was a nunatak region in the southern Prealps during Pleistocene glaciations. Later range expansion of extant populations was limited by poor seed dispersal. We also analyzed the cpDNA trnL-F intergenic spacer to check whether the sequence is useful for studying the phylogenetic relationships of Hladnikia within the family Apiaceae (Umbelliferae). Our results support the assertion that H. pastinacifolia is an old taxon.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2012
• Tom: 54
• Numer: 1
• Opis fizyczny: p.84-96,fig.,ref.

Twórcy

autor

Sajna N.

• Biology Department, Faculty of Natural Sciences and Matematics, University of Maribor, Koroska c. 160, SI - 2000 Maribor, Slovenia

autor

Kavar T.

autor

Sustar-Vozlic J.

autor

Kaligaric M.

Bibliografia

• AJANI Y, AJANI A, CORDES MJ, WATSON MF, and DOWNIE RS. 2008. Phylogenetic analysis of nrDNA ITS sequencesreveals relationships within five groups of IranianApiaceae subfamily Apioideae. Taxon 57: 383–401.
• ALTSCHUL SF, MADDEN TL, SCHAFFER AA, ZHANG J, ZHANG Z, MILLER W, and LIPMAN DJ. 1997. Gapped BLAST andPSI-BLAST: A new generation of protein database searchprograms. Nucleic Acids Research 25: 3389–3402.
• AYRES DR, and RYAN FJ. 1999. Genetic diversity and construction of the narrow endemic Wyethia reticulata andits congener W. bolanderi (Asteraceae) using RAPD andallozyme techniques. American Journal of Botany 86:344–353.
• COLE TC. 2003. Genetic variation in rare and common plants. Annual Review of Ecology, Evolution, and Systematics 34: 213–237.
• CORREGGIARI A, ROVERI M, and TRINCARDI F. 1996. Late Pleistocene and Holocene evolution of the north Adriaticsea. Quaternario 9: 697–704.
• CRUDEN RW. 1976. Variation in pollen-ovule ratios and nectar secretion – Preliminary evidence of ecotypic adaptation.Annals of the Missouri Botanical Garden 63: 277–289.
• ÈUŠIN B. 2004. Hladnikia pastinacifolia Rchb. – rebrinèevolistna hladnikija, hladnikovka. In: Èušin B [ed.], Natura 2000 v Sloveniji – Rastline. ZRC SAZU,Ljubljana.
• ĆWIKLIŃSKA A, BRODA Z, BOCIANOWSKI J, and DOBRZYCKA A. 2010. The usefulness of RAPD and AFLP markers for determininggenetic similarity in rye (Secale L.) species andsubspecies. Acta Biologica Cracoviensia SeriesBotanica 52: 19–25.
• DAKSKOBLER I. 1999. Contribution to the knowledge of the association Fraxino orni-Pinetum nigrae Martin-Bosse 1967. Wissenschaftliche Mitteilungen aus dem Niederösterreichischen Landesmuseum 12: 25–52.
• DAKSKOBLER I. 2006. Calcareous open sedge swards and stony grasslands (Seslerietalia caeruleae) on the northernedge of the Trnovski gozd plateau (the DinaricMountains, western Slovenia). Hacquetia 5: 73–112.
• DAVIS PH. 1950. Cliff vegetation in the eastern Mediterranean. Journal of Ecology 39: 63–93.
• DESPRES L, LORIOT S, and GAUDEUL M. 2002. Geographic pattern of genetic variation in the European globeflowerTrollius europaeus L. (Ranunculaceae) inferred fromamplified fragment length polymorphism markers.Molecular Ecology 11: 2337–2347.
• DOWNIE SR, WATSON MF, SPALIK K, and KATZ-DOWNIE DS. 2000. Molecular systematics of Old World Apioideae (Apiaceae): relationships among some members of tribe Peucedaneae sensu lato, the placement of several islandendemicspecies, and resolution within the apioid superclade.Canadian Journal of Botany 78: 506–528.
• FALCONER DS, and MACKAY TFC. 1996. Introduction to Quantitative Genetics. Longman, Harlow, Essex.
• FELSENSTEIN J. 2004. PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department ofGenome Sciences, University of Washington, Seattle.
• FLEISCHMANN A. 1844. Übersicht der Flora Krain's. Laibach. GASTON KJ. 1997. What is rarity? In: Kunin WE, Gaston KJ[eds.], The Biology of Rarity, Causes andConsequences of Rare-Common Differences.Chapman & Hall, London.
• GAUDEUL M, TILL-BOTTRAUD I, BARJON F, and MANEL S. 2004. Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers. Heredity 92: 508–518.
• GITZENDANNER MA, and SOLTIS PA. 2000. Patterns of genetic variation in rare and widespread congeners. American Journal of Botany 87: 783–792.
• HEGI G. 1975. Illustrierte Flora von Mitteleuropa 5(2). Paul Parey, Berlin, Hamburg. JIMENEZ JF, SANCHEZ-GOMEZ P, GUEMES J, WERNER O, and ROSSELLO JA. 2002. Genetic variability in a narrow endemic snapdragon (Antirrhinum subbaeticum, Scrophulariaceae) using RAPD markers. Heredity 89:387–393.
• KIM H, and CHANG CS. 2005. The genetic variation in geographically peripheral populations of Bupleurumeuphorbioides (Apiaceae) and comparisons of geneticvariation with a congener, B. longiradiatum. IntegrativeBiosciences 9: 27–35.
• KIMURA M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120.
• KRAUS FB, WOLF S, and MORITZ RFA. 2009. Male flight distance and population substructure in the bumblebee Bombus terrestris. Journal of Animal Ecology 78:247–252.
• KRUCKENBERG AR, and RABINOWITZ D. 1985. Biological aspects of endemism in higher plants. Annual Review of Ecology, Evolution, and Systematics 16: 447–479.
• KUMAR S, TAMURA K, and NEI M. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5: 150–163.
• KUNIN WE, and GASTON KJ. 1997. The Biology of Rarity. Causes and Consequences of Rare-Common Differences. Chapman & Hall, London. LOHSE K, NICHOLLS JA, and STONE GN. 2011. Inferring the colonisation of a mountain range – refugia vs. nunatak survival in high alpine ground beetles. MolecularEcology 20: 394–408.
• LOPEZ-PUJOL J, BOSCH M, SIMON J, and Blanche C. 2002. Allozyme variation and population structure of the verynarrow endemic Seseli farrenyi (Apiaceae). BotanicalJournal of the Linnean Society 138: 305–314.
• MAMELI G, FILIGHEDDU R, BINELLI G, and MELONI M. 2008. The genetic structure of the remnant populations ofCentaurea horrida in Sardinia and associated islands.Annals of Botany – London 101: 633–640.
• MAYER E. 1960. Endemiène cvetnice obmoèja jugovzhodnih apneniških Alp, njihovega predgorja in ilirskega prehodnegaozemlja. In: Lazar J [ed.], Zbornik ob 150. letnicibotaniènega vrta v Ljubljani, SAZU, Ljubljana.
• MEDAIL F, and DIADEMA K. 2009. Glacial refugia influence plant diversity patterns in the Mediterranean Basin. Journal ofBiogeography 36: 1333–1345.
• MELIK A. 1960. Slovenija – Slovensko Primorje 2(4). Slovenska matica, Ljubljana.
• OIKI S, KAWAHARA T, INOUE K, OHARA M, and MAKI M. 2001. Random amplified polymorphic DNA (RAPD) variationamong populations of the insular endemic plantCampanula microdonta (Campanulaceae). Annals ofBotany – London 87: 661–667.
• PAWLOWSKI B. 1970. Remarques sur l'endemisme dans la flore des Alpes et des Carpates. Vegetatio 21: 181–243.
• PEAKALL R, and SMOUSE PE. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.
• PERKO D. 2001. Analiza Površja Slovenije s Stometrskim Digitalnim Modelom Reliefa. ZRC SAZU, Ljubljana.
• PFOSSER M, JAKUBOWSKY G, SCHLUTER PM, FER T, KAT H, STUESSY TF, and SUN B-Y. 2006. Evolution ofDystaenia takesimana (Apiaceae), endemic to UllungIsland, Korea. Plant Systematics and Evolution 256:159–170.
• PLUNKETT GM, and DOWNIE SR. 1999. Major lineages within Apiaceae subfamily Apioideae: a comparison of chloroplast restriction site and DNA sequence data. American Journal of Botany 86:1014–1026.
• REICHENBACH HGL. 1830–1833. Flora Germanica Excursoria. Lipsiae. RUNEMARK H. 1969. Reproductive drift, a neglected principle in reproductive biology. Botaniska Notiser 122: 90–129.
• ŠAJNA N, KUŠAR P, SLANA NOVAK L, and NOVAK T. 2009. Notes on thermo- and hygropreference in Leiobunum roseum C.L. KOCH, 1839 (Opiliones: Sclerosomatidae) in a habitat of Hladnikia pastinacifolia Reichenbach, 1831 (Spermatophyta: Apiaceae). Contributions to NaturalHistory 12: 1111–1123.
• ŠAJNA N, KUŠAR P, SLANA NOVAK L, and NOVAK T. 2011. Benefits of low-intensive grazing: co-occurrence of umbelliferousplant (Hladnikia pastinacifolia Rchb.) and opilionidspecies (Phalangium opilio L.) in dry, calcareous grassland.Polish Journal of Ecology 59: 777–786.
• ŠERA B, FERAKOVA V, KOCIANOVA E, VAGENKNECHT V, MAJZLAN O, and DUBRAVCOVA Z 2005. Peucedanum arenarium subsp. arenarium – a critically endangered species of the Slovak flora (morphometry, distribution, biology). Biologia 60: 17–23.
• SCHNEEWEISS GM, and SCHONSWETTER P. 2011. A re-appraisal of nunatak survival in arctic-alpine phylogeography. Molecular Ecology 12: 190–192.
• SCHONSWETTER P, TRIBSCH A, STEHLIK I, and NIKLFELD H. 2004. Glacial history of high alpine Ranunculus glacialis (Ranunculaceae) in the European Alps in a comparative phylogeographical context. Biological Journal of the Linnean Society 81: 183–195.
• STEHLIK I, SCHNELLER JJ, and BACHMANN K. 2002. Immigration and in situ glacial survival of the low-alpine Erinus alpinus(Scrophulariaceae). Biological Journal of theLinnean Society 77: 87–103.
• SUN F-J, and DOWNIE SR 2004. A molecular systematic investigation of Cymopterus and its allies (Apiaceae) based onphylogenetic analyses of nuclear (ITS) and plastid (rps16intron) DNA sequences. South African Journal ofBotany 70: 407–416.
• SUN F-J, DOWNIE SR, and HARTMAN LR. 2004. An ITS-based phylogenetic analysis of the perennial endemic Apiaceae subfamily Apioideae of western North America. Systematic Botany 29: 419–431.
• SURINA B. 2001. Fitocenološke raziskave jelovo-bukovega gozda (Omphalodo-Fagetum s. lat.) v zahodnem delu ilirske florne province. Master thesis. University of Ljubljana, Biotechnical Faculty, Ljubljana.
• SURINA B. 2002. Phytogeographical differentiation in the Dinaric fir-beech forest (Omphalodo-Fagetum s.lat.) ofthe western part of the Illyrian floral province. ActaBotanica Croatica 61: 145–178.
• SURINA B, and VREŠ B. 2009. The association Drepanoclado uncinati-Heliospermetum pusilli (Arabidetalia caeruleae, Thlaspietea rotundifolii) in the Trnovski gozd plateau (Slovenia, NW Dinaric Mts). Hacquetia 8: 31–40.
• SUŠNIK F. 1964. Taksonomska in horološka problematika taksona Hladnikia pastinacifolia Rchb. Ph.D. dissertationUniversity of Ljubljana, Ljubljana.
• ŠUŠTAR-VOZLIÈ J, and JAVORNIK B. 1999. Genetic relationship in cultivars of hop, Humulus lupulus L., determined by RAPD analysis. Plant Breeding 118: 175–181.
• TABERLET P, GIELLY L, PATTOU G, and BOUVET J. 1991. Universal primers for amplification of three non-codingregions of chloroplast DNA. Plant Molecular Biology 17:1105–1109.
• THOMPSON JD, GIBSON TJ, PLEWNIAK F, JEANMOUGIN F, and HIGGINS DG. 1997. The ClustalX windows interface: flexiblestrategies for multiple sequence alignment aided byquality analysis tools. Nucleic Acids Research 25: 4876–4882.
• TORRES E, IRIONDO JM, and PEREZ C. 2003. Genetic structure of an endangered plant, Antirrhinum microphyllum (Scrophulariaceae): allozyme and RAPD analysis. American Journal of Botany 90: 85–92.
• TRIBSCH A, and SCHONSWETTER P. 2003. Patterns of endemism and comparative phylogeography confirm palaeo-environmental evidence for Pleistocene refugia in the Eastern Alps. Taxon 52: 477–497.
• VILATERSANA R, SUSANNA A, and BROCHMANN C. 2007. Genetic variation in Femeniasia balearica (Compositae, Cardue), an endemic and endangered monotypic genus from the Balearic Islands (Spain). Botanical Journal of the Linnean Society 153: 97–107.
• VOGEL JC, RUMSEY FJ, SCHNELLER JJ, BARRETT JA, and GIBBY M. 1999. Where are the glacial refugia in Europe? Evidencefrom pteridophytes. Biological Journal of the LinneanSociety 66: 23–37.
• WALTHER-HELLWIG K, and FRANKL R. 2000. Foraging distances of Bombus muscorum, Bombus lapidarius, and Bombusterrestris (Hymenoptera, Apidae). Journal of InsectBehavior 13: 239–246.
• WEISING K, NYBOM H, WOLLF K, and KAHL G. 2005. DNA Fingerprinting in Plants. Principles, Methods, and Applications. CRC Press, Boca Raton.
• WESTERGAARD KB, ALSOS IG, POPP M, ENGELSKJON T, FLATBERG KI, and BROCHMANN C. 2011. Glacial survival may matter after all: nunatak signatures in the rare European populations of two west-arctic species. Molecular Ecology 20: 376–393.
• WRABER T. 1990. Èaven, ein botanisch beruhmter Berg in Slowenien. Carinthia 180: 195–210.

Uwagi

Rekord w opracowaniu