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2017 | 66 | 1 |

Tytuł artykułu

Vital staining of bacteria by sunset yellow pigment

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture with sunset yellow. Our results suggest that sunset yellow is suitable pigment to dye microorganisms.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

66

Numer

1

Opis fizyczny

p.113-117,fig.,ref.

Twórcy

autor
  • Beijing Institute of Biotechnology, Beijing, PR China
  • School of Life Sciences, AnHui University, AnHui, PR China
autor
  • Departments of Laboratory, Yuhuangding Hospital, Shandong, PR China
autor
  • Beijing Institute of Biotechnology, Beijing, PR China
  • Institute of Health Service and Medical Information, Academy of Military Medical Sciences, Beijing, PR China
autor
  • Beijing Institute of Biotechnology, Beijing, PR China
autor
  • Beijing Institute of Biotechnology, Beijing, PR China
  • School of Life Sciences, AnHui University, AnHui, PR China
autor
  • Beijing Institute of Biotechnology, Beijing, PR China
autor
  • Departments of Laboratory, Yuhuangding Hospital, Shandong, PR China
autor
  • Beijing Institute of Biotechnology, Beijing, PR China
  • School of Life Sciences, AnHui University, AnHui, PR China

Bibliografia

  • Adler A., S. Navon-Venezia, J. Moran-Gilad, E. Marcos, D. Schwartz and Y. Carmeli. 2011. Laboratory and clinical evaluation of screening agar plates for detection of carbapenem-resistant Enterobacteriaceae from surveillance rectal swabs. J. Clin. Microbiol. 49: 2239–2242.
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  • Cohen H.W., R.M. Gould and V.W. Sidel. 2004. The pitfalls of bioterrorism preparedness: the anthrax and smallpox experiences. Am. J. Pub. Health. 94: 1667–1671.
  • Conrad T.M., M. Frazier, A.R. Joyce, B.-K. Cho, E.M. Knight, N.E. Lewis, R. Landick and B.Ø. Palsson. 2010. RNA polymerase mutants found through adaptive evolution reprogram Escherichia coli for optimal growth in minimal media. Proceedings of the National Academy of Sciences. 107: 20500–20505.
  • Cox S., C. Mann, J. Markham, H. Bell, J. Gustafson, J. Warmington and S. Wyllie. 2000. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 88: 170–175.
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  • Ferreira L., F. Sánchez-Juanes, M. González-Ávila, D. Cembrero-Fuciños, A. Herrero-Hernández, J.M. González-Buitrago and J.L. Muñoz-Bellido. 2010. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48: 2110–2115.
  • Jünger M., W. Vautz, M. Kuhns, L. Hofmann, S. Ulbricht,J.L. Baumbach, M. Quintel and T. Perl. 2012. Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria. Appl. Microbiol. Biotechnol. 93: 2603–2614.
  • Nevado J.B., J.R. Flores, C.G. Cabanillas, M.V. Llerena andA.C. Salcedo. 1998. Resolution of ternary mixtures of Tartrazine, Sunset Yellow and Ponceau 4R by derivative spectrophotometric ratio spectrum-zero crossing method in commercial foods. Talanta 46: 933–942.
  • Ngwa G., R. Schop, S. Weir, C. León-Velarde and J. Odumeru. 2013. Detection and enumeration of E. coli O157: H7 in water samples by culture and molecular methods. J. Microbiol. Meth. 92: 164–172.
  • Pérez-Díaz I. and R. McFeeters. 2009. Modification of azo dyes by lactic acid bacteria. J. Appl. Microbiol. 107: 584–589.
  • Poul M., G. Jarry, M.O. Elhkim and J.-M. Poul. 2009. Lack of genotoxic effect of food dyes amaranth, Sunset Yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. Food Chem. Tox. 47: 443–448.
  • Salleh F.M., A.M. Al-Mekhlafi, A. Nordin, A.M. Yasin, H.M. Al-Mekhlafi and N. Moktar. 2011. Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting microsporidial spores in fecal specimens. Diagn. Microbiol. Infect. Dis. 69:82–85.
  • Salleh F.M., T.S. Anuar, A.M.Yasin and N. Moktar. 2012. Wintergreen oil: a novel method in Wheatley’s trichrome staining technique. J. Microbiol. Meth. 91: 174–178.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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