PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 55 | 3 |

Tytuł artykułu

Phenotypic and molecular characterization of the causal agent of chafer beetle mortality in the wheat fields of the Kurdistan province, Iran

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We report the first case of chafer beetle [Anisoplia austriaca (Herbst 1783)] mortality caused by Actinomucor elegans var. elegans in wheat fields of the Kurdistan province, Iran. For three years, dead larvae of Anisoplia austriaca were collected from wheat fields of the Kurdistan province. Similar isolates of a fast-growing fungus were recovered from all samples. The fungal isolates were identified as A. elegans var. elegans based on morphological and cultural characteristics. The identity of the species was further confirmed using sequence data of the ITS (Internal Transcribed Spacer) region of ribosomal DNA. Koch’s postulates were fulfilled by the inoculation of the larvae of A. austriaca and Galleria mellonella (Linnaeus, 1758) (as the model insect) using the spore suspension of A. elegans var. elegans. The viability of sporangiospores was evaluated using a spore dilution technique on germination medium. The results on the pathogenicity (100% mortality in A. austriaca larvae) and viability tests (germination: 95.45%) demonstrated that A. elegans var. elegans can be considered as a potential biocontrol agent against the chafer beetle. Field experiments are still required to evaluate the capacity of A. elegans as a biological control agent.

Wydawca

-

Rocznik

Tom

55

Numer

3

Opis fizyczny

p.227-234,fig.,ref.

Twórcy

autor
  • Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
autor
  • Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
autor
  • Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
autor
  • Plant Protection Department, Agricultural and Natural Resources Research Center of Kurdistan, P.O. Box 714, Sanandaj, Iran

Bibliografia

  • Arzanlou M., Groenewald J.Z., Gams W., Braun U., Shin H.D., Crous P.W. 2007. Phylogenetic and morphotaxonomic revision of Ramichloridium and allied genera. Studies in Mycology 58: 57–93.
  • Batra L.R., Batra S.W.T., Bohart G.E. 1973. The mycoflora of domesticated and wild bees (Apoidea). Mycopathologia et Mycologia Applicata 49 (1): 13–44.
  • Benjamin C.R., Hesseltine C.W. 1957. The genus Actinomucor. Mycologia 49: 240–249.
  • Costa H.S., Robb K.L., Wilen C.A. 2001. Increased persistence of Beauveria bassiana spore viability under high ultravioletblocking greenhouse plastic. HortScience 36 (6): 1082–1084.
  • Cotter G., Doyle S., Kavanagh K. 2000. Development of an insect model for the in vivo pathogenicity testing of yeasts. FEMS Immunology & Medical Microbiology 27 (2): 163–169.
  • Davel G., Featherston P., Fernández A., Abrantes R., Cristina C., Rodero L., Sztern C., Perrotta D. 2001. Maxillary sinusitis caused by Actinomucor elegans. Journal of Clinical Microbiology 39 (2): 740–742.
  • de Hoog G.S., Gerrits van den Ende A.H.G. 1998. Molecular diagnostics of clinical strains of filamentous Basidiomycetes. Mycoses 41 (5–6): 183–189.
  • Degawa Y., Gams W. 2004. A new species of Mortierella, and an associated sporangiiferous mycoparasite in a new genus, Nothadelphia. Studies in Mycology 50 (2): 567–572.
  • Domsch K.H., Gams W., Anderson T.H. 2007. Compendium of Soil Fungi. IHW-Verlag, Eching, Netherlands, 43 pp.
  • Han B.Z., Ma Y., Rombouts F.M., Nout M.J.R. 2003. Effects of temperature and relative humidity on growth and enzyme production by Actinomucor elegans and Rhizopus oligosporus during sufu pehtze preparation. Food Chemistry 81 (1): 27–34.
  • Herlinda S. 2010. Spore density and viability of entomopathogenic fungal isolates from Indonesia, and their virulence against Aphis gossypii Glover (Homoptera: Aphididae). Trop Life Sciences Results 21 (1): 11–19.
  • Hoffmann K., Pawłowska J., Walther G., Wrzosek M., de Hoog G.S., Benny G.L., Kirk P.M., Voigt K. 2013. The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies. Persoonia 30: 57–76.
  • Jameson M., Mico E., Galante E. 2007. Evolution and phylogeny of the scarab subtribe Anisopliina (Coleoptera: Scarabaeidae: Rutelinae: Anomalini). Systematic Entomology 32 (3): 429–449.
  • Jong S.C., Yuan G.F. 1985. Actinomucor taiwanensis sp. nov., for manufacture of fermented soybean food. Mycotaxon 23: 261–264.
  • Khan Z.U., Ahmad S., Mokaddas E., Chandy R., Cano J., Guarro J. 2008. Actinomucor elegans var. kuwaitiensis isolated from the wound of a diabetic patient. Antonie Van Leeuwenhoek 94 (3): 343–352.
  • Klein M.G., Kaya H.K. 1995. Bacillus and Serratia a species for scarab control. Memórias do Instituto Oswaldo Cruz 90 (1): 87–95.
  • Mahmud A., Lee R., Munfus-McCray D., Kwiatkowski N., Subramanian A., Neofytos D., Carroll K., Zhang S.X. 2012. Actinomucor elegans as an emerging cause of Mucormycosis. Journal of Clinical Microbiology 50 (3): 1092–1095.
  • Milner R.J., Huppatz R J., Swaris S.C. 1991. A new method for assessment of germination of Metarhizium conidia. Journal of Invertebrate Pathology 57 (1): 121–123.
  • Möller E.M., Bahnweg G., Geiger H.H. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Results 20 (22): 6115–6116.
  • Phalip V., Hatsch D., Laugel B., Jeltsch J.M. 2006. An overview of fungal community diversity in diseased hop plantations. FEMS Microbiology Ecology 56 (2): 321–329.
  • Rehner S.A., Samuels G.J. 1994. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98 (6): 625–634.
  • Soetopo D. 2004. Efficacy of selected Beauveria bassiana (Bals.) Vuill. isolates in combination with a resistant cotton variety (PSB-Ct 9) against the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Ph.D. thesis, University of the Philippines Los Banos, Philippines.
  • Sun B.D., Liu X.Z. 2008. Occurrence and diversity of insect-associated fungi in natural soils in China. Applied Soil Ecology 39 (1): 100–108.
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28 (10): 2731–2739.
  • Tully C.C., Romanelli A.M., Sutton D.A., Wickes B.L., Hospenthal D.R. 2009. Fatal Actinomucor elegans var. kuwaitiensis infection following combat trauma. Journal of Clinical Microbiology 47 (10): 3394–3399.
  • Vilgalys R., Hester M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246.
  • Waldorf A.R., Diamond R.D. 1985. Neutrophil chemotactic responses induced by fresh and swollen Rhizopus oryzae spores and Aspergillus fumigatus conidia. Infection and Immunity 48 (2): 458–463.
  • Walther G., Pawłowska J., Alastruey-Izquierdo A., Wrzosek M., Rodriguez-Tudela J.L., Dolatabadi S., Chakrabarti A., de Hoog G.S. 2013. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30: 11–47.
  • White T.J., Bruns T.D., Lee S.B., Taylor J.W. 1990. Amplification and sequencing of fungal ribosomal RNA genes for phylogenetics. p. 315–322. In: “PCR-Protocols and Applications, a Laboratory Manual” (N. Innis, D. Gelfand, J. Sninsky, T.C. White, eds.). Academic Press, New York, USA.
  • Zheng R.Y., Liu X.Y. 2005. Actinomucor elegans var. meitauzae, the correct name for A. taiwanensis and Mucor meitauzae (Mucorales, Zygomycota). Nova Hedwigia 80 (3–4): 419–431.
  • Zoberi M.H., Grace J.K. 1990. Fungal associated with the subterranean termite Reticulitermes flavipes in Ontario. Mycologia 82 (3): 289–294.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-4464909d-d913-4cd6-b350-2f3d1f6f1e78
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.