The soybean gene, GmMYBJ2, encodes a R2R3-type transcription factor involved in drought stress tolerance in Arabidopsis thaliana

• Autorzy: Su L.-T. , Wang Y. , Liu D.-Q. , Li X.-W. , Zhai Y. , Sun X. , Li X.-Y. , Liu Y.-J. , Li J.-W. , Wang Q.-Y.
• Języki publikacji: EN

Abstrakty

EN

MYB genes are extensively distributed in higher plants and constitute one of the largest transcription factors (TFs) families. These TFs have been proved to be implicated in the regulation of plant growth, development, metabolism, and multiple abiotic stress responses. In the present study, a new soybean MYB gene, denoted GmMYBJ2, was isolated and its function was characterized. The GmMYBJ2 cDNA is 1428 bp in length with an open reading frame (ORF) of 960 bp encoding 319 amino acids. Sequence and yeast one-hybrid analyses showed GmMYBJ2 contains two MYB domains and belongs to R2R3-MYB protein with transactivation activity. Transient expression analysis using the GmMYBJ2-GFP fusion gene in onion epidermal cells showed GmMYBJ2 protein is targeted to the nucleus. GmMYBJ2 was induced by drought, cold, salt, and exogenous abscisic acid (ABA). Arabidopsis overexpressing GmMYBJ2 exhibited a higher seed germination rates (GRs), a notable increase in the soluble sugar content under water-deficit stress, and a lower water loss rate (WLR) when water is sufficient. These results indicated the overexpression of GmMYBJ2 make transgenic Arabidopsis more tolerant to drought stress than wild-type (WT) plants, and GmMYBJ2 may be useful for improving drought stress tolerance in transgenic plant breeding.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 07
• Opis fizyczny: fig.,ref.

Twórcy

autor

Su L.-T.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Wang Y.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Liu D.-Q.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Li X.-W.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Zhai Y.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Sun X.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Li X.-Y.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Liu Y.-J.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Li J.-W.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

autor

Wang Q.-Y.

• Jilin Key Laboratory for Crop Genetic Engineering, College of Plant Science, Jilin University, Changchun, 130062, Jilin, China

Bibliografia

• Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78
• Ahuja I, de Vos RC, Bones AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci15:664–674
• Bailey RW (1958) Reaction of pentoses with anthrone. Biochem J 68:669–672
• Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
• Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T, Vuylsteke M, Leonhardt N, Dellaporta SL, Tonelli C (2005) A guard-cellspecific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol 15:1196–1200
• Cominelli E, Sala T, Calvi D, Gusmaroli G, Tonelli C (2008) Overexpression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability. Plant J 53:53–64
• Dai X, Xu Y, Ma Q, Xu W, Wang T, Xue Y, Chong K (2007) Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought and salt stress in transgenic Arabidopsis. Plant Physiol 143:1739–1751
• Dedio W (1975) Water relations in wheat leaves as screening tests for drought resistance. Can J Plant Sci 55:369–378
• Du H, Zhang L, Liu L, Tang XF, Yang WJ, Wu YM, Huang YB, Tang YX (2009) Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry 74:1–11
• Du H, Yang SS, Liang Z, Feng BR, Liu L, Huang YB, Tang YX (2012) Genome-wide analysis of the MYB transcription factor superfamily in soybean. BMC Plant Biol 12:106
• Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E, Debeaujon I, Routaboul JM, Alboresi A, Weisshaar B, Lepiniec L (2008) MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana. Plant J 55:940–953
• Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573–581
• Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33:751–763
• Fu J (1995) A breeding report on new soybean cultivar of Jilin32. J Jilin Agric Sci 2:18–20 (in Chinese)
• Ganesan G, Sankararamasubramanian HM, Harikrishnan M, Ashwin G, Parida A (2012) A MYB transcription factor from the grey mangrove is induced by stress and confers NaCl tolerance in tobacco. J Exp Bot 63:4549–4561
• Gray J (2005) Guard cells: transcription factors regulate stomatal movements. Curr Biol 15:R593–R595
• Haga N, Kato K, Murase M, Araki S, Kubo M, Demura T, Suzuki K, Müller I, Voss U, Jürgens G, Ito M (2007) R1R2R3-Myb proteins positively regulate cytokine sis through activation of KNOLLE transcription in Arabidopsis thaliana. Development 134:1101–1110
• Hamanishi ET, Thomas BR, Campbell MM (2012) Drought induces alterations in the stomatal development program in Populus. J Exp Bot 63:4959–4971
• He CY, Zhang JS, Chen SY (2002) A soybean gene encoding a proline-rich protein is regulated by salicylic acid, an endogenous circadian rhythm and by various stresses. Theor Appl Genet 104:1125–1131
• He Y, Li W, Lv J, Jia Y, Wang M, Xia G (2012) Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J Exp Bot 63:1511–1522
• Hu H, You J, Fang Y, Zhu X, Qi Z, Xiong L (2008) Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Mol Biol 67:169–181
• Ito M (2005) Conservation and diversification of three-repeat Myb transcription factors in plants. J Plant Res 118:61–69
• Kang JY, Choi HI, Im MY, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357
• Karimi M, Inzé D, Depicker A (2002) GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci 7:193–195
• Kerepesi I, Galiba G (2000) Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Sci 40:482–487
• Kiyosue T, Yamaaguchi-Shinozaki K, Shinozaki K (1994) Characterization of two cDNAs (ERD10 and ERD14) corresponding to genes that respond rapidly to dehydration stress in Arabidopsis thaliana. Plant Cell Physiol 35:225–231
• Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141
• Letunic I, Copley RR, Pils B, Pinkert S, Schultz J, Bork P (2006) SMART 5: domains in the context of genomes and networks. Nucleic Acids Res 34:D257–D260
• Li XW, Li JW, Zhai Y, Zhao Y, Zhao X, Zhang HJ, Su LT, Wang Y, Wang QY (2013) A R2R3-MYB transcription factor, GmMYB12B2, affects the expression levels of flavonoid biosynthesis genes encoding key enzymes in transgenic Arabidopsis plants. Gene 532:72–79
• Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS, Chen SY (2008a) Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta 228:225–240
• Liao Y, Zou HF, Wang HW, Zhang WK, Ma B, Zhang JS, Chen SY (2008b) Soybean GmMYB76, GmMYB92, and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants. Cell Res 18:1047–1060
• Lippold F, Sanchez DH, Musialak M, Schlereth A, Scheible WR, Hincha DK, Udvardi MK (2009) AtMyb41 regulates transcriptional and metabolic responses to osmotic stress in Arabidopsis. Plant Physiol 149:1761–1772
• Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406
• Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods 25:402–408
• Luo GZ, Wang HW, Huang J, Tian AG, Wang YJ, Zhang JS, Chen SY (2005) A putative plasma membrane cation/proton antiporter from soybean confers salt tolerance in Arabidopsis. Plant Mol Biol 59:809–820
• Mare C, Mazzucotelli E, Crosatti C, Francia E, Stanca AM, Cattivelli L (2004) Hv-WRKY38: a new transcription factor involved in cold and drought-response in barley. Plant Mol Biol 55:399–416
• Matsui K, Umemura Y, Ohme-Takagi M (2008) AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. Plant J 55:954–967
• Nakashima K, Ito Y, Yamaguchi-Shinozaki K (2009) Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol 149:88–95
• Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. Plant J 34:137–148
• Niu C, Wei W, Zhou QY, Tian AG, Hao YJ, Zhang WK, Ma B, Lin Q, Zhang ZB, Zhang JS, Chen SY (2012) Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants. Plant, Cell Environ 5:1156–1170
• Onate-Sanchez Anderson JP, Young J, Singh KB (2007) AtERF14, a member of the ERF family of transcription factors, plays a nonredundant role in plant defense. Plant Physiol 143(1):400–409
• Pesch M, Hulskamp M (2009) One, two, three models for trichome patterning in Arabidopsis? Curr Opin Plant Biol 12:587–592
• Qin YX, Wang MC, Tian YC, He WX, Han L, Xia GM (2012) Overexpression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis. Mol Biol Rep 39:7183–7192
• Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110
• Sakuma Y (2006) Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18:1292–1309
• Seki M, Umezawa T, Urano K, Shinozaki K (2007) Regulatory metabolic networks in drought stress responses. Curr Opin Plant Biol 10:296–302
• Simon M, Lee MM, Lin Y, Gish L, Schiefelbein J (2007) Distinct and overlapping roles of single-repeat MYB genes in root epidennal patterning. Dev Biol 311:566–578
• Simpson SD, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. Plant J 33:259–270
• Song SY, Chen Y, Chen J, Dai XY, Zhang WH (2011) Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress. Planta 234:331–345
• Su LT, Li JW, Liu DQ, Zhai Y, Zhang HJ, Li XW, Zhang QL, Wang Y, Wang QY (2014) A novel MYB transcription factor, GmMYBJ1, from soybean confers drought and cold tolerance in Arabidopsis thaliana. Gene 538:46–55
• Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
• Valliyodan B, Nguyen HT (2006) Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Curr Opin Plant Biol 9:189–195
• Vannini C, Locatelli F, Bracale M, Magnani E, Marsoni M, Osnato M, Mattana M, Baldoni E, Coraggio I (2004) Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants. Plant J 37:115–127
• Vinocur B, Altman A (2005) Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol 16:123–132
• Wang YJ, Li YD, Luo GZ, Tian AG, Wang HW, Zhang JS, Chen SY (2005) Cloning and characterization of an HDZip I gene GmHZ1 from soybean. Planta 221:831–843
• Weston K (1998) Myb proteins in life, death and differentiation. Curr Opin Genet Dev 8:76–81
• Yamaguchi-Shinozaki K, Shinozaki K (1993) Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants. Mol Gen Genet 236:331–340
• Yang A, Dai XY, Zhang WH (2012) A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot 63:2541–2556
• Zhai Y, Wang Y, Li Y, Lei T, Yan F, Su L, Li XW, Zhao Y, Sun X, Li J, Wang QY (2012) Isolation and molecular characterization of GmERF7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco. Gene 513:174–183
• Zhai Y, Li JW, Li XW, Lei TT, Yan F, Zhao Y, Li YJ, Su LT, Wang Y, Wang QY (2013) Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean. Biol Plantarum 57:26–32
• Zhang LC, Zhao GY, Xia C, Jia JZ, Liu X, Kong XY (2012) A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. J Exp Bot 63:5873–5885
• Zhu L, Guo JS, Zhu J, Zhou C (2014) Enhanced expression of EsWAX1 improves drought tolerance with increased accumulation of cuticular wax and ascorbic acid in transgenic Arabidopsis. Plant Physiol Biochem 75:24–35

Identyfikatory

DOI

10.1007/s11738-015-1889-5