Factors influencing the restitution of the duodenal and colonic mucosa after damage

• Autorzy: Riegler M. , Feil W. , Wenzl E. , Schiessel R.
• Języki publikacji: EN

Abstrakty

EN

Rapid epithelial restitution is an important protective mechanism which enables the gastrointestinal mucosa to reestablish epithelial integrity following superficial injury within hours. In this study we examined the influence of an acidic luminal pH, removal of the necrotic layer, nutrient bicarbonate, calcium and sodium desoxycholate (Na-DOC) on restitution in the rabbit duodenum in vitro and the role of Na-DOC and calcium for rapid restitution of the human colon in vitro. Transmucosal potential difference (PD), short-circuit current (lsc) were measured and resistance against passive ion flux (R) was calculated. Electrophysiological changes paralleled morphological injury but did not necessarily reflect restitution in all experiments. The extent of mucosal injury was assessed by computerized real-time morphometry. 5 hrs after luminal exposure to 10 mH HCl for 10 min residual damage (RD) was 14% in the duodenum. Luminal pH of 3.0 (RD of 30%), removal of necrotic layer at acidic luminal pH (RD of 66%), absence of bicarbonate from the serosal solution (RD of 35 % at neutral luminal pH; RD of 96% at acidic luminal pH) and removal of calcium from the serosal solution (RD of 58 %) impaired restitution in the duodenum. Continuous postinjury luminal Na-DOC exposure did not influence restitution in the duodenum (RD of 19%). 5 hrs after luminal exposure to 0.5 mM Na-DOC for 10 min RD was 26% in the human colon. Continuous postinjury luminal Na-DOC exposure (RD of 51 %) and removal of calcium from the nutrient solution (RD of 65 %) impaired restitution in the human colon. Thus we conclude that restitution of the rabbit duodenum in vitro requires a necrotic layer and bicarbonate flux to withstand acidic luminal pH, while restitution is not Effected by Na-DOC. In the human colon Na-DOC inhibits restitution. Both the duodenum and colon require calcium for rapid restitution.

Słowa kluczowe

EN

gastrointestinal tract; duodenal mucosa; colonic mucosa; physiological condition; restitution; damage; sodium deoxycholate; calcium; morphometry; rabbit

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1991
• Tom: 42
• Numer: 1
• Opis fizyczny: p.61-71,fig.,ref.

Twórcy

autor

Riegler M.

• Vienna General Hospital, University Clinic of Surgery I, Alser Strasse 4, A-1090 Vienna, Austria

autor

Feil W.

• Vienna General Hospital, University Clinic of Surgery I, Alser Strasse 4, A-1090 Vienna, Austria

autor

Wenzl E.

• Vienna General Hospital, University Clinic of Surgery I, Alser Strasse 4, A-1090 Vienna, Austria

autor

Schiessel R.

• Vienna General Hospital, University Clinic of Surgery I, Alser Strasse 4, A-1090 Vienna, Austria

Bibliografia

• 1. Riecken EO, Stallmach A, Zeitz M, Schulzke JD, Menge H, Gregor M. Growth and transformation of the small intestinal mucosa-importance of connective tissue, gut associated lymphoid tissue and gastrointestinal regulatory peptides. Gut 1989; 30: 1630-1640.
• 2. Morris GP, Wallace JL, Harding PL, Krausse EJ, Lolle SJ. Correlations between changes in indicators of gastric mucosal barrier integrity at time of exposure to “barrier breakers” and extent of hemorrhagic erosions one hour later. Dig Dis Sci 1984; 29: 6-11.
• 3. Flemström G, Garner A. Gastroduodenal НСОЗ-transport: characteristics and proposed role in acidity regulation and mucosal protection. Am J Physiol 1982; 242: G183-G193.
• 4. Wenzl E. Feil W, Starlinger M, Schiessel R. Alkaline secretion. A protective mechanism against acid injury in rabbit duodenum. Gastroenterology 1987; 92 : 709-715.
• 5. Feil W, Klimesch S, Karner P, Wenzl E, Starlinger M, Lacy ER, Schiessel R. Importance of an alkaline microenvironment for rapid restitution of the rabbit duodenal mucosa in vitro. Gastroenterology 1989; 97: 112-122.
• 6. Svanes K, Ito S, Takeuchi K, Silen W. Restitution of the surface epithelium of the vitro frog gastric mucosa after damage with hyperosmolar sodium chloride. Gastroenterology 1982; 82: 1409-1426.
• 7. Lacy ER, Ito S. Rapid epithelial restitution of the rat gastric mucosa after ethanol injury. Lab Invest 1984; 51: 573-583.
• 8. Feil W, Wenzl E, Vattay P, Starlinger M, Sogukoglu T, Schiessel R. Repair of rabbit duodenal mucosa after acid injury in vivo and in vitro. Gastroenterology 1987; 92: 1973-1986.
• 9. Hudspeth AJ. The recovery of local transepithelial resistance following single-cell lesions. Exp Cell Res 1982; 138: 331-342.
• 10. Donowitz M, Madara JL. Effect of extracellular calcium depletion on epithelial structure and function in rabbit ileum: a model for selective crypt or villus epithelial cell damage and suggestion of secretion by villus epithelial cells. Gastroenterology 1982; 83: 1231-1243.
• 11. Feil W, Lacy E, Wong YM, Burger D, Wenzl E, Starlinger M, Schiessel R. Rapid epithelial restitution of the human and rabbit colonic mucosa. Gastroenterology 1989; 97: 685-701.
• 12. Critchow J, Magee D, Ito S, Takeuchi K, Silen W. Requirements for restitution of the surface epithelium of frog stomach after mucosal injury. Gastroenterology 1983; 88: 237-249.
• 13. Deitch EA. The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure. Arch Surg 1990; 125: 403-404.