PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 33 | 6 |
Tytuł artykułu

Involvement of phospholipase A2 in the response of Solanum species to an elicitor from Phytophtora infestans

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Changes in activity of phospholipase A₂ (PLA₂), a key enzyme in lipid metabolism and signal network in defence mechanisms, were investigated in Solanum species and Phytophthora infestans interaction. We have compared PLA₂ activity in response to an elicitor, a culture filtrate (CF) derived from P. infestans, in non-host resistant Solanum nigrum var. gigantea, field resistant S. tuberosum cv Bzura and susceptible S. tuberosum clone H-8105. To elucidate the contribution of specific forms of PLA₂ to plant defence mechanism reasonably selective PLA₂ inhibitors, haloenol lactone suicide substrate (HELSS) and p-bromophenacyl bromide (BPB), which discriminate between Ca⁺²-independent PLA₂ (iPLA₂) and Ca⁺²-dependent secretory PLA₂ (sPLA₂), were used. The in vivo and in vitro effects of the inhibitors on PLA₂ activity and on generation of reactive oxygen species (ROS) induced by CF in the studied plants were assayed. We found that PLA₂ activity increased in response to CF treatment, displaying various kinetics and intensity depending on the resistance status of a given genotype. Differences among the genotypes in the effects of each inhibitor on CF-induced PLA₂ activity and on ROS production may reflect the diversity of PLA₂ isoforms in plants. Contrary to BPB, the inhibitory effect of HELSS was observable mainly on CF-induced PLA₂ activity, which suggests that iPLA₂ participates in signal transduction in defence reactions. Various effects of the two inhibitors on PLA₂ activity and ROS production suggest different contribution of sPLA₂ and iPLA₂ to modulation of defence reactions in the interaction between Solanum genotypes and P. infestans.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
33
Numer
6
Opis fizyczny
p.2521-2531,fig.,ref.
Twórcy
  • Department of Plant Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
autor
  • Department of Plant Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
  • Department of Plant Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
Bibliografia
  • Baker CJ, Orlandi EW (1995) Active oxygen in plant pathogenesis. Ann Rev Phytopathol 33:299–321
  • Balboa MA, Balsinde J (2006) Oxidative stress and arachidonic acid mobilization. Biochim Biophys Acta 1761:385–391
  • Balsinde J, Balboa MA, Insel PA, Dennis EA (1999) Regulation and inhibition of phospholipase A₂. Annu Rev Pharmacol Toxicol 39:175–189
  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254
  • Cacas J-L, Vailleau F, Davoine C, Ennar N, Agnel J-P, Tronchet M, Ponchet M, Blein J-P, Roby D, Triantaphylidès Ch, Montillet J-L (2005) The combined action of 9 lipoxygenase and galactolipase is sufficient to bring about programmed cell death during tobacco hypersensitive response. Plant Cell Environ 28:1367–1378
  • Chandra S, Heinstein PF, Low PS (1996) Activation of phospholipase A by plant defense elicitors. Plant Physiol 110:979–986
  • Dana R, Malech HL, Levy R (1994) The requirement for phospholipase A₂ for activation of the assembled NADPH oxidase in human neutrophils. Biochem J 297:217–223
  • Dhondt S, Gouzerh G, Müller A, Legrand M, Heitz T (2002) Spatiotemporal expression of patatin-like lipid acyl hydrolases and accumulation of jasmonates in elicitor-treated tobacco leaves are not affected by endogenous levels of salicylic acid. Plant J 32:749–762
  • Doke N (1983) Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiol Plant Pathol 23:345–357
  • Göbel C, Feussner I, Schmidt A, Scheel D, Sanchez-Serrano J, Hamberg M, Rosahl S (2001) Oxylipin profiling reveals the preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells. J Biol Chem 276:6267–6273
  • Han WK, Sapirstein A, Hung CC, Alessandrini A, Bonventre JV (2003) Cross-talk between cytosolic phospholipase A₂a (cPLA₂a) and secretory phospholipase A₂ (sPLA₂) in hydrogen peroxide-induced arachidonic acid release in murine mesangial cells. J Biol Chem 278:24153–24163
  • Hazen SL, Zupan LA, Weiss RH, Getman DP, Gross RW (1991) Suicide inhibition of canine myocardial cytosolic calciumindependent phospholipaseA₂. J Biol Chem 266:7227–7232
  • Holk A, Rietz S, Zahn M, Quader H, Scherer GFE (2002) Molecular identification of cytosolic, patatin-related phospholipases A from Arabidopsis with potential functions in plant signal transduction. Plant Physiol 130:90–101
  • Jiménez-Atiénzar M, Cabanes J, Gandía-Herrero F, Escribano J, García-Carmona F, Pérez-Gilabert M (2003) Determination of the phospholipase activity of patatin by a continuous spectrophotometric assay. Lipids 38:677–682
  • Kawakita K, Senda K, Doke N (1993) Factors affecting in vitro activation of potato phospholipase A₂. Plant Sci 92:183–190
  • Kim YJ, Kim KP, Han SK, Munoz NM, Zhu X, Sano H, Leff AR, Cho W (2002) Group V phospholipase A₂ induces leukotriene biosynthesis in human neutrophils through the activation of group IVA phospholipase A₂. J Biol Chem 277:36479–36488
  • La Camera S, Balagué C, Göbel C, Geoffroy P, Legrand M, Feussner I, Roby D, Heitz T (2009) The Arabidopsis patatin-like protein 2 (PLP2) plays an essential role in cell death execution and differentially affects biosynthesis of oxylipins and resistance to pathogens. Mol Plant Microbe Interact 22:469–481
  • La Camera S, Geoffroy P, Samaha H, Ndiaye A, Rahim G, Legrand M, Heitz T (2005) A pathogen-inducible patatin-like lipid acyl hydrolase facilitates fungal and bacterial host colonization in Arabidopsis. Plant J 44:810–825
  • Lee HY, Bahn SC, Shin JS, Hwang I, Back K, Doelling JH, Ryu SB (2005) Multiple forms of secretory phospholipase A₂ in plants. Prog Lipid Res 44:52–67
  • Levy R (2006) The role of cytosolic phospholipase A₂-a in regulation of phagocytic functions. Biochim Biophys Acta 176:1323–1334
  • Mansfeld J, Ulbrich-Hofmann R (2007) Secretory phospholipase A₂-a from Arabidopsis thaliana: functional parameters and substrate preference. Chem Phys Lipids 150:156–166
  • Mueller MJ, Berger S (2009) Reactive electrophilic oxylipins: pattern recognition and signalling. Phytochemistry 70:1511–1521
  • Munnik T, Irvine RF, Musgrave A (1998) Phospholipid signalling in plants. Biochim Biophys Acta 1389:222–272
  • Mur LAJ, Kenton P, Lloyd AJ, Ougham H, Prats E (2008) The hypersensitive response; the centenary is upon us but how much do we know? J Exp Bot 59:501–520
  • Narváez-Vásquez J, Florin-Christensen J, Ryan CA (1999) Positional specificity of a phospholipase A activity induced by wounding, systemin, and oligosaccharide elicitors in tomato leaves. Plant Cell 11:2249–2260
  • Piedras P, Hammond-Kosack KE, Harrison K, Jones JDG (1998) Rapid, Cf-9- and Avr9-dependent production of active oxygen species in tobacco suspension cultures. Mol Plant Microbe Interact 11:1155–1166
  • Polkowska-Kowalczyk L, Wielgat B, Maciejewska U (2004) The elicitor-induced oxidative processes in leaves of Solanum species with differential polygenic resistance to Phytophthora infestans. J Plant Physiol 161:913–920
  • Polkowska-Kowalczyk L, Montillet J-L, Agnel J-P, Triantaphylidès C, Wielgat B, Maciejewska U (2008) Changes in the initial phase of lipid peroxidation induced by elicitor from Phytophthora infestans in Solanum species. J Plant Physiol 165:1929–1939
  • Ryu SB (2004) Phospholipid-derived signalling mediated by phospholipase A in plants. Trends Plant Sci 9:229–235
  • Scherer GFE (2002) Secondary messengers and phospholipase A₂ in auxin signal transduction. Plant Mol Biol 49:357–372
  • Scherer GFE, Ryu SB, Wang X, Matos AR, Heitz T (2010) Patatinrelated phospholipase A: nomenclature, subfamilies and functions in plants. Trends Plant Sci 15:693–700
  • Senda K, Yoshioka H, Doke N, Kawakita K (1996) A cytosolic phospholipase A₂ from potato tissues appears to be patatin. Plant Cell Physiol 37:347–353
  • Tian ZD, Liu J, Wang BL, Xie CH (2006) Screening and expression analysis of Phytophthora infestans induced genes in potato leaves with horizontal resistance. Plant Cell Rep 25:1094–1103
  • Viehweger K, Dordschbal B, Roos W (2002) Elicitor-activated phospholipase A₂ generates lysophosphatidylcholines that mobilize the vacuolar H⁺ pool for pH signaling via the activation of Na⁺-dependent proton fluxes. Plant Cell 14:1509–1525
  • Vleeshouwers VGAA, van Dooijeweert W, Gouvers F, Kamoun S, Colon LT (2000) The hypersensitive response is associated with host and nonhost resistance to Phytophthora infestans. Planta 210:853–864
Uwagi
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-42b4a2dc-f736-408b-bf6a-18e762242c1e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.