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2016 | 25 | 2 |

Tytuł artykułu

Pollutant removal and PCR-DGGE analysis of microbial community structural changes in an integrated vertical-flow constructed wetland

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In this study we measured changes in the CODCr, TN, NH4+-N, TP, and DO concentrations and the pH of different layers in an integrated vertical-flow constructed wetland (IVCW) treating eutrophic water. We also examined the composition and diversity of the bacterial community in the IVCW using PCR-DGGE. Our results showed that most of the contaminants were removed during sewage flow from the down-flow chamber to the up-flow chamber. The removal rates of TN, TP, CODCr, and NH4+-N were, respectively, 63.7%, 66.7%, 72.2%, and 67.9% in the down-flow; consequently, the CODCr and TP concentrations of the effluent fell below 10 mg/L and 0.05 mg/L. There were some common microorganisms and specific microorganisms in the different layers of the constructed wetland because of the changes in environmental factors and nutrient levels. The diversity of the bacterial community was highest in the upper layer of the down-flow system, and the similarity in the down-flow system was higher than that in the up-flow system. From the down-flow system to the up-flow system, the diversity of the bacterial community increased from 2.31 to 1.95 and the structural similarity gradually increased from 65.7% to 70.9%. The higher complexity of the bacterial community in the upper layer of the constructed wetland may be supported by plant oxygenation, rhizodeposition, and litter accumulation.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

25

Numer

2

Opis fizyczny

p.709-715,fig.,ref.

Twórcy

autor
  • School of Civil Engineering and Architecture, East China JiaoTong University, Nanchang 330013, China
autor
  • School of Civil Engineering and Architecture, East China JiaoTong University, Nanchang 330013, China
autor
  • School of Civil Engineering and Architecture, East China JiaoTong University, Nanchang 330013, China
autor
  • School of Civil Engineering and Architecture, East China JiaoTong University, Nanchang 330013, China
autor
  • School of Civil Engineering and Architecture, East China JiaoTong University, Nanchang 330013, China

Bibliografia

  • 1. RAGUSA S.R., MCNEVIN D., QASEM S. Indicators of biofilm development and activity in constructed wetlands microcosms. Water Res. 38 (12), 2865, 2004.
  • 2. FU R.B., YANG H.Z., GU G.W., ZHANG Z. Analysis of Substrate Microorganisms Status in Constructed wetlands and Their Correlation with Pollutants Removal for Waste-water Treatment. Research of Environmental Sciences. 18, 44, 2005.
  • 3. MENG P., PEI H., HU W., SHAO Y., LI Z. How to increase microbial degradation in constructed wetlands: Influencing factors and improvement measures. Bioresource technology. 157, 316, 2014.
  • 4. VACCA G., WAND H., NIKOLAUSZ M., KUSCHK P., KÄSTNER M. Effect of plants and filter materials on bacteria removal in pilot-scale constructed wetlands. Water research. 39 (7), 1361, 2005.
  • 5. WERKER A.G., DOUGHERTY J.M., MCHENRY J.L., VAN LOON W.A. Treatment variability for wetland waste-water treatment design in cold climates. Ecological Engineering, 19 (1), 1, 2002.
  • 6. BALÁZS M., RÓNAVÁRI A., NÉMETH A., BIHARI Z., RUTKAI E., BARTOS P., KISS I., SZVETNIK A. Effect of DNA polymerases on PCR-DGGE patterns. International Biodeterioration & Biodegradatio. 84, 244, 2013.
  • 7. MAGDALENA K., THOMAS B., NARYTTZAN. DIAZB, ALEXANDER G., SEBASTIAN J., LUTZ K., DIMITRI MILLERA, KAI J. RUNTEB, PRISCA V., ALFRED P., ANDREAS S. Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing. J. Biotechnol. 142, 38, 2009.
  • 8. XIA S.Q., JIA R.Y., FENG F., XIE K., LI H.X., JING D.F., XU X.T. Effect of solids retention time on antibiotics removal performance and microbial communities in an A/O-MBR process. Bioresource technology. 106, 36, 2012.
  • 9. SHEN P.H., ZHANG J.Y., ZHANG J., JIANG C.J., TANG X.L., LI J.F., ZHANG M., WU B. Changes in microbial community structure in two anaerobic systems to treat bagasse spraying wastewater with and without addition of molasses alcohol wastewater. Bioresource technology. 131, 333, 2013.
  • 10. APHA. Standard Methods for the Examination of Water and Wastewater, 19th ed'. American Public Health Association. Washington DC, 1995.
  • 11. VARELA A.R., ANDRÉ S., NUNES et al. Insights into the relationship between antimicrobial residues and bacterial populations in a hospital-urban wastewater treatment plant system. Water research. 54, 327, 2014.
  • 12. MOBARRY B.K., WAGNER M., URBAIN V., RITTMANN B.E., STAHL D.A. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Applied and Environmental Microbiology. 62 (6), 2156, 1996.
  • 13. ADRADOS B., SÁNCHEZ O., ARIAS C.A., BECARESD E., GARRIDOC L., MASC J., BRIXB H., MORATÓA J. Microbial communities from different types of natural wastewatertreatment systems: Vertical and horizontal flow constructed wetlands and biofilters. Water research. 55, 304, 2014.
  • 14. HUANG J., WANG S.H., LUO W.G., LU Y. Influence of plant photosynthetic characteristics on DO distribution, purification effect in constructed wetlands. Acta Scientiae Cir-cumstantiae. 26, 1828, 2006.
  • 15. SU J.F., MA F., WANG H.Y. Application of PCR-DGGE to resolvemicrobial diversity in bioceramic reactor. Acta Scientiae Circumstantiae. 27 (3), 386, 2007.
  • 16. IASUR-KRUH L., HADAR Y., MILSTEIN D., GASITH A., MINZ D. Microbial population and activity in wetland microcosms constructed for improving treated municipal wastewater. Microbial ecology. 59 (4), 700, 2010.
  • 17. SI Y.M., LIU Y.J., WU Y.K, LI C.R. Different Wastewater Treatment Processes and Analysis of the Changes in Micro-bial Community Structure in It. Journal of Southwest University (in China ), 34 (8), 122, 2012.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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