Cation-permeable vacuolar channels in the tonoplast of Marchantia polymorpha

• Autorzy: Kupisz K. , Dziubinska H. , Trebacz K.
• Języki publikacji: EN

Abstrakty

EN

The object of our patch-clamp study was the liverwort Marchantia polymorpha. Basic solutions were symmetrical in the bath and in the pipette and contained 100 mM KCl and 0.5 mM CaCl2. Whole vacuole recordings mainly showed slowly activating outward rectifying currents, typical for SV (slowvacuolar) channels. The unitary conductance registered from isolated patches was 74.7±4.0 pS at 100 mV. Replacement of K+ with Na+ caused reduction of the unitary conductance to 59.8±6.87 pS. Tenfold decrease of the KCl shifted the reversal potential close to EK and caused reduction of the SV unitary conductance to 31.5±5.5 pS. Moreover, the gradient of KCl revealed the current at the negative potential; the unitary conductance at −100 mV was 43.3±0.5 pS, whereas the gradient of Na+ did not evoke such an effect. This may suggest that inwardly rectifying K+ channels exist in the tonoplast of the liverwort. Supported by NCN grant 2013/09/B/NZ1/01052

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: p.338

Twórcy

autor

Kupisz K.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

autor

Dziubinska H.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

autor

Trebacz K.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland