Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

• Autorzy: Bochniarz M , Wawron W. , Szczubial M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the mechanisms of staphylococcal resistance to methicillin. CNS (n=100 isolates) were prepared from the mammary inflammatory secretions of 86 cows from farms located in the Lublin region. Methicillin-resistant isolates constituted 20.0% of all CNS. Staphylococcus sciuri (n=8) and Staphylococcus xylosus (n=6) were most abundant, followed by Staphylococcus chromogenes (n=3), Staphylococcus haemolyticus (n=2) and Staphylococcus warneri (n=1). The mecA gene was found in 50.0% of MRCNS (10.0% of all CNS isolates) belonging to two species: S. sciuri and S. xylosus. All mecA-positive isolates contained the protein of low affinity to penicillin (penicillin-binding protein 2a – PBP2a). The enzyme hydrolysing the β-lactam ring in antibiotics was detected in 40.0% of MRCNS; 10.0% of MRCNS isolates were characterised by the presence of the mecA gene and ability to produce β-lactamase. The remaining 20.0% of MRCNS isolates showing phenotypic resistance to methicillin were mecA gene-negative and were not able to produce β-lactamase.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 4
• Opis fizyczny: p.687-692,fig.,ref.

Twórcy

autor

Bochniarz M

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

autor

Wawron W.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

autor

Szczubial M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

Bibliografia

• Aarestrup FM, Wegener HC, Rosdahl VT, Jensen NE (1995) Staphylococcal and other bacterial species associated with intramammary infections in Danish dairy herds. Acta Vet Scand 36: 475-487.
• Bartoszewicz-Potyrała M, Przondo-Mordarska A (2002) Cechy gronkowców koagulazoujemnych warunkujące ich chorobotwórczość. Postępy Mikrobiologii 41: 351-366.
• Bochniarz M, Wawron W (2011) Antibiotic susceptibility of methicillin-resistant and methicillin-susceptible coagulase-negative staphylococci isolated from bovine mastitis. Pol J Vet Sci 14: 405-410.
• Brakstad OG, Maeland JA (1997) Mechanisms of methicillin resistance in staphylococci. APMIS 105: 264-276.
• Chaffer M, Leitner G, Winkler M, Glickman A, Krifucks O, Ezra E, Saran A (1999) Coagulase-negative staphylococci and mammary gland infections in cows. Zentralbl Veterinarmed B 46: 707-712.
• Chambers HF (1997) Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 10: 781-791.
• Clinical and Laboratory Standards Institute (CLSI) (2002) Performance standards for antimicrobial disk susceptibility tests for bacteria isolated from animals. NCCLS document M32-A2, Aproved standard. 2nd ed. CLSI, Wayne, PA.
• Contreras A, Sanchez A, Corrales JC, Sierra D, Gonzalo C, Paape MJ (2005) Mastitis in small ruminants. In: Hogeween H (ed) Mastitis in dairy production. Wageningen Academic Publishers. The Netherlands, pp 67-74.
• Deinhofer M, Pernthaner A (1995) Staphylococcus spp. as mastitis-related pathogens in goat milk. Vet Microbiol 43: 161-166.
• Devriese LA, Baele M, Vaneechoutte M, Martel A, Haesebrouck F (2002) Identification and antimicrobial susceptibility of Staphylococcus chromogenes isolated from intramammary infections of dairy cows. Vet Microbiol 87: 175-182.
• Gentilini E, Denamiel G, Betancor A, Rebuelto M, Rodriguez Fermepin M, De Torrest RA (2002) Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina. J Dairy Sci 85: 1913-1917.
• Hackbarth CJ, Chambers HF (1989) Methicillin-resistant staphylococci: genetics and mechanisms of resistance. Antimicrob Agents Chemother 33: 991-994.
• Honkanen-Buzalski T, Myllys V, Pyörälä S(1994) Bovine clinical mastitis due to coagulase-negative staphylococci and their susceptibility to antimicrobials. Zentralbl Veterinarmed B 41: 344-350.
• Horstkotte MA, Knobloch JK, Rohde H, Mack D (2001) Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a – specific latex agglutination test. J Clin Microbiol 39: 3700-3702.
• Hussain M, Wilcox MH, White PJ (1993) The slime of coagulase-negative staphylococci: biochemistry and relation to adherence. FEMS Microbiol Rev 10: 191-207.
• Hussain Z, Stoakes L, Garrow S, Longo S, Fitzgerald V, Lannigan R (2000) Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test. J Clin Microbiol 38: 2051-2054.
• Hussain Z, Stoakes L, John MA, Garrow S, Fitzgerald V (2002) Detection of methicillin resistance in primary blood culture isolates of coagulase-negative staphylococci by PCR, slide agglutination, disk diffusion, and a commercial method. J Clin Microbiol 40: 2251-2253.
• Louie L, Majury A, Goodfellow J, Louie M, Simor AE (2001) Evaluation of a latex agglutination test (MRSA-Screen) for detection of oxacillin resistance in coagulase-negative staphylococci. J Clin Microbiol 39: 4149-4151.
• Luthje P, Schwarz S (2006) Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother 57: 966-969.
• Łopaciuk U, Dzierżanowska D (2002) Gronkowce metycylinooporne: mechanizmy oporności, czynniki zjadliwości oraz metody genotypowania. Postępy Mikrobiologii 41: 401-418.
• Makovec JA, Ruegg PL (2003) Results of milk samples submitted for microbiological examination in Wisconsin from 1994 to 2001. J Dairy Sci 86: 3466-3472.
• Malinowski E, Lassa H, Kłossowska A, Smulski S, Markiewicz H, Kaczmarowski M (2006) Etiological agents of dairy cows’ mastitis in western part of Poland. Pol J Vet Sci 9: 191-194.
• Miller MB, Meyer H, Rogers E, Gilligan PH (2005) Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing and mecA Real-Time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus. J Clin Microbiol 43: 3450-3452.
• Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS, Koo HC (2007) Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J Dairy Sci 90: 1176-1185.
• Myllys V, Asplund K, Brofeldt E, Hirvela-Koski V, Honkanen-Buzalski T, Junttila J, Kulkas L, Myllykangas O, Niskanen H, Saloniemi H, Sandholm M, Saranpaa T (1998) Bovine mastitis in Finland in 1988 and 1995 – changes in prevalence and antimicrobial resistance. Acta Vet Scand 39: 119-126.
• Pitkälä A, Haveri M, Pyörälä S, Myllys V, Honkanen-Buzalski T (2004) Bovine mastitis in Finland 2001 – prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci 87: 2433-2441.
• Pyörälä S, Taponen S (2009) Coagulase-negative staphylococci – emerging mastitis pathogens. Vet Microbiol 134: 3-8.
• Rajala-Schultz PJ, Smith KL, Hogan JS, Love BC (2004) Antimicrobial susceptibility of mastitis pathogens from first lactation and older cows. Vet Microbiol 102: 33-42.
• Sawant AA, Gillespie BE, Oliver SP (2009) Antimicrobial susceptibility of coagulase-negative Staphylococcus species isolated from bovine milk. Vet Microbiol 134: 73-81.
• Suzuki E, Hiramatsu K, Yokota T (1992) Survey of methicillin – resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution. Antimicrob Agents Chemother 36: 429-434.
• Taponen S, Koort J, Bjorkroth J, Saloniemi H, Pyörälä S (2007) Bovine intramammary infections caused by coagulase-negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism – based analysis. J Dairy Sci 90: 3301-3307.
• Van Duijkeren E, Box AT, Heck ME, Wannet WJ, Fluit AC (2004) Methicillin-resistant staphylococci isolated from animals. Vet Microbiol 103: 91-97.
• Waller KP, Aspan A, Nyman A, Persson Y, Andersson UG (2011) CNS species and antimicrobial resistance in clinical and subclinical bovine mastitis. Vet Microbiol 152: 112-116.
• York MK, Gibbs L, Chehab F, Brooks GF (1996) Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase negative staphylococci. J Clin Microbiol 34: 249-253.