Evaluation of Wondfo Rapid Diagnostic Kit (Pf-HRP2/PAN-pLDH) for diagnosis of malaria by using nano-gold Immunochromatographic assay

• Autorzy: Wu J. , Peng Y. , Liu X. , Li W. , Tang S.
• Języki publikacji: EN

Abstrakty

EN

Prompt and accurate diagnosis is necessary to start adequate treatment for different affecting species including P. falciparum and P. vivax. Here we described the Wondfo Rapid diagnostic Kit (Pf-HRP2/PAN-pLDH) for the detection of P. falciparum and pan-plasmodium in patient specimen by using a nano-gold immunochromatographic assay. Our rapid assay adapted nano-gold labeling techniques and the monoclonal antibodies (mAbs) against both histidine rich protein-2 (Pf HRP-2) of P. falciparum and pan plasmodium-specific pLDH (pan pLDH). The established two-antibody sandwich immunochromatographic assay could detect P. falciparum and pan-plasmodium. The sensitivity and specificity of Wondfo rapid diagnostic kit were determined by comparing with the “gold standard” of microscopic examination of blood smears. In this study1023 blood samples were collected from outpatient clinics in China and Burma, and detected by both Wondfo kit and microscopic examination. The detection sensitivity and specificity of Wondfo rapid diagnostic kit were 96.46% and 99.67% for P. falciparum (HRP2), 95.03% and 99.24% for pLDH, 96.83% and 99.74% for non-falciparum species, 96.70% and 99.74% for P. vivax, respectively. These results indicate that Wondfo rapid diagnostic assay may be useful for detecting P. falciparum and non-P. falciparum (especially P.v.) in patient specimen.

Słowa kluczowe

EN

malaria; Plasmodium falciparum; Plasmodium vivax; rapid diagnostic Kit; immunochromatographic method; monoclonal antibody; infectious disease

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 2
• Opis fizyczny: p.267-271,fig.,ref.

Twórcy

autor

Wu J.

• Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China

autor

Peng Y.

• School of Food and Bioengineering, South China University of Technology, Guangzhou, Guangdong, 510640, China

autor

Liu X.

• Guangzhou Wondfo Biotech. Co., Ltd., Guangzhou Accurate and Correct Test Co., Ltd. Guangzhou, Guangdong, 510643, China

autor

Li W.

• School of Food and Bioengineering, South China University of Technology, Guangzhou, Guangdong, 510640, China

autor

Tang S.

• Guangzhou Wondfo Biotech. Co., Ltd., Guangzhou Accurate and Correct Test Co., Ltd. Guangzhou, Guangdong, 510643, China

Bibliografia

• Ashley E.A., Touabi M., Ahrer M., Hutagalung R., Htun K., Luchavez J., Dureza C., Proux S., Leimanis M., Lwin M.M., Koscalova A., Comte E., Hamade P., Page A.L., Huang S.H. 2006. Gold nanoparticle-based immunochromatographic test for identification of Staphylococcus aureus from clinical specimens. Clinica Chimica Acta, 373, 139–143. DOI: 10.1016/j.cca.2006.05.026.
• Bell D., Wongsrichanalai C., Barnwell J.W. 2006. Ensuring quality and access for malaria diagnosis: how can it be achieved? Nature Reviews Microbiology, 4, S7–S20. DOI: 10.1038/nrmicro1525.
• Lwanga S.K., Lemeshow S. 1991. Sample size determination in health studies — a practical manual. Geneva, Switzerland: World Health Organization.
• Noppadon T., Chatnapa D., Polrat W., Srivicha K. 2009. Malaria Diagnosis: A Brief Review. Korean Journal of Parasitology, 47, 93–102. DOI: 10.3347/kjp.2009.47.2.93.
• Peng Y., Wu J., Wang J., Li W., Yu S. 2012. Study and evaluation of Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for diagnosis of Plasmodium falciparum. Parasitology Research, 110, 1421–1425. DOI:10.1007/s00436-011-2643-6.
• Reyburn H., Mbita R., Drakely C., Carneiro I., Mwakasungula E., Mwerinde O., Saganda K., Shao J., Kitua A., Olomi R., Greenwood B.M., Whitty C.J. 2004. Overdiagnosis of malaria patients with severe febrile illness in Tanzania: a prospective study. British Medical Journal, 329, 2121. DOI: 10.1136/bmj.38251.658229.55.
• WHO. 1996. WHO information consultation on recent advances in diagnostic techniques and vaccines for malaria: a rapid dipstick antigen capture assay for the diagnosis of falciparum malaria. Bulletin of the World Health Organization, 74, 47–54.
• WHO. 2006. Guidelines for the treatment of malaria. 1st ed. Geneva, Switzerland. World Health Organization, 133–143.
• WHO. 2008. List of known commercially available antigen-detecting malaria RDTs. Available at: http://www.wpro.who.int/sites/rdt.
• WHO. 2009a. Malaria Rapid Diagnostic Test Performance. Results of WHO product testing of malaria RDTs: Round 2, World Health Organization.
• WHO. 2009b. Methods for surveillance of antimalarial drug efficacy. Global malaria programme. Geneva, Switzerland: World Health Organization.
• WHO. 2010. Guidelines for the treatment of malaria, 2nd edn. World Health Organization, Geneva.
• WHO. 2013. World Malaria Report 2013. World Health Organization.
• Wongsrichanalai C., Barcus M.J., Muth S., Sutamihardja A., Wernsdorfer W.H. 2007. A review of malaria diagnostic tools: microscopy and rapid diagnostic test (RDT). American Journal of Tropical Medicine and Hygiene, 77(6 Suppl.), 119–127.