Valproic acid but not minocycline alleviates striatal neuron degeneration in the rat model of nitracerebral hematoma

• Autorzy: Majak K. , Kowianski P. , Dziewiatkowski J. , Wojcik S. , Morys J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: The study evaluates the effect of experimentally induced intracerebral haematoma (ICH) on density of neurodegenerating neurons and volume of the rat striatum. In addition, we studied how administration of valproic acid and minocycline, the two drugs generally believed to be neuroprotective agents, influences the density of these neurons. METHODS: 80 adult male Wistar rats were assign to one of 4 experimental groups: control, (CON), haematoma (HAEM), haematoma and minocycline (MINO) and haematoma and valproic acid (VAL). Five animals from each experimental group were sacrificed at four time points: 2 weeks, 4 weeks, 24 weeks and 48 weeks after ICH. To assess the neurodegeneration the sections were stained with FluoroJadeB (FJB). The CellSense Dimension 1.5 (Olympus, Japan) image analysis system was employed to measure the densities of FJB-labelled neurons as well as volume of the striatum. Statistical analyses were performed using two way ANOVA with replications. To find differences between groups post-hoc Tukey test was applied. A P-value <0.05 was considered significant. RESULTS: Our study revealed that valproic acid treatment decreased the density of FJB-labeled degenerating neurons 4 weeks after ICH induction in the ipsilateral striatum. Minocycline did not have such effect. None of the two drugs influenced the volume of the striatum. CONCLUSIONS: Our study revealed that valproic acid alleviates striatal neuron degeneration in the rat model of ICH. Because striatum has been implicated to play role in motor control as well as cognition and affective control, the valproic acid treatment might have beneficial effects on these processes after ICH.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S99-S100

Twórcy

autor

Majak K.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Kowianski P.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Dziewiatkowski J.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Wojcik S.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland

autor

Morys J.

• Department of Anatomy and Neurobiology, Medical University of Gdansk, Gdansk, Poland