Changes in plasma membrane aquaporin gene expression under osmotic stress and blue light in tomato

• Autorzy: Balarynova J. , Danihlik J. , Fellner M.
• Języki publikacji: EN

Abstrakty

EN

Divergent abiotic stresses induce osmotic stress on plant cells resulting in an imbalance in water homeostasis which is preserved by aquaporins. Since the plasma membrane aquaporins (PIPs) were shown to be involved in seed development and responses to abiotic stresses, we focused on determining the contribution of mannitol-induced osmotic stress, blue light (BL), and 7B-1 mutation to their gene expression in tomato (Solanum lycopersicum L.) seeds. To assess that, we used a quantitative RT-PCR to determine the expression profiles of genes encoding PIPs. Subsequently, a multiple linear regression analysis was used to evaluate the impact of studied stressors (mannitol and BL) and 7B-1 mutation on PIP gene expressions. We found that mannitol-induced osmotic stress and 7B-1 mutation (conferring the lower responsiveness to osmotic stress- and BL-induced inhibition of seed germination) decreased expression of PIP1;3, PIP2;3 and PIP1;2, PIP2;1 genes, respectively. This might be a way to retain water for radicle elongation and seed germination under the stress conditions. Interestingly, the expression of PIP1;3 gene was downregulated not only by osmotic stress, but also by BL. Altogether, our data indicate the existence of a link between osmotic stress and BL signalling and the involvement of the 7B-1 mutation in this crosstalk.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 02
• Opis fizyczny: Article 27 [8p.], fig.,ref.

Twórcy

autor

Balarynova J.

• Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, Slechtitelu 27, 7837 Olomouc, Czech Republic

autor

Danihlik J.

• Department of Biochemistry, Faculty of Science, Palacky University, Slechtitelu 27, 78371 Olomouc, Czech Republic

autor

Fellner M.

• Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, Slechtitelu 27, 7837 Olomouc, Czech Republic

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Identyfikatory

DOI

10.1007/s11738-017-2602-7