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2008 | 58 | 4 |

Tytuł artykułu

Effect of native enzymes on the quality of lipids in experimentally-stored rapeseed

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Activity and immunoreactivity of lipase, activity of lipoxygenase, acid and peroxide values were determined during the storage of rapeseed with 6, 10, 14, and 18% moisture content. The most intensive changes in lipase activity were observed during storage of rapeseed with 10% moisture content and ranged from 18.73 to 55.85 A.U./mg protein as compared to stored rapeseed with 6, 14, and 18% moisture content. During the storage there were also observed changes in lipase immunoreactivity, lipoxygenase activity, acid and peroxide values, however none of the relationships between enzymes and quality of rapeseed lipids was found to be statistically significant and therefore it can be concluded that the processes taking place during storage of rapeseed with varied moisture content show complexity and the enzymes examined in the experiment can appear to be not only factors affecting the quality of the lipid fraction.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

58

Numer

4

Opis fizyczny

p.471-476,fig.,ref.

Twórcy

autor
  • Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

Bibliografia

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  • 2. Belguith H., El Khil H.K., Fattouch S., Jridi T., Ben Hamida J., Contribution of blotting techniques to the study of rapeseeds (Brassica napus L.) lipases. Electrophoresis, 2001, 22, 18–22.
  • 3. Bradford M.M., A rapid and sensitive method for the quantitation for microgram quantities of protein utilizing the principle of protein – dye binding. Anal. Biochem., 1976, 72, 248–254.
  • 4. Fauconnier M.L., Vanzeveren E., Marlier M., Lognay G., Wathelet J.P., Severin M., Assessment of lipoxygenase activity in seed extracts from 35 plant species. Grasas y Aceites, 1995, 46, 6–10.
  • 5. Gardner W.H., Biological roles and biochemistry of the lipoxygenase pathway. Hort Sci., 1995, 30, 197–205.
  • 6. Grabska J., Jędrychowski L., Miaczynska A., Application of immunoblotting method to detect native lipases of rapeseed. Pol. J. Food Nutr. Sci., 1997, 6, 107–114.
  • 7. Hills M.J., Murphy D.J., Characterization of lipases from lipid bodies and microsomal membranes of erucic acid free oilseed rape (Brassica napus L.) cotyledons. Biochem J., 1988, 249, 687–693.
  • 8. Hills M.J., Beevers H., An antibody to the castor bean glyoxysomal lipase (62kDa) also binds to a 62 kD protein in extracts from many young oilseed plants. Plant Physiol., 1987, 85, 1084–1088.
  • 9. James J.J., Lakshmi B.S., Raviprasad V., Ananth M.J., Kangueane P., Gautam P., Insights from molecular dynamics simulations into pH-dependent enantioselective hydrolysis of ibuprofen esters by Candida rugosa lipase. Protein Engin., 2003, 16, 1017–1024.
  • 10. Khalyafa A., Kermasha S., Alli I., Partial purification and characterization of lipoxygenase of canola seed (Brassica napus var. Westar). J. Agric. Food Chem., 1990, 38, 2003–2008.
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  • 12. Lin Y.H., Huang A.H.C., Lipase in lipid bodies of cotyledons of rape and rape and mustard seedlings. Arch. Biochem. Biophys., 1983, 225, 360–369.
  • 13. Lima V.M.G., Krieger N., Mitchell D.A., Fontana J.D., Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents. Biochem. Engin. J., 2004, 18, 65–71.
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  • 15. Meshehdani T., Pokorny J., Davidek J., Panek J., Deactivation of lipoxygenases during rapeseed processing. Corps Gras, 1990b, 37, 23–27.
  • 16. Polish Standard PN 74/R-66165, Grain of oil plants. Determination of the acid value of fat (in Polish).
  • 17. Polish Standard PN 84/A-86918. Edible vegetable fats. Testing methods. Determination of peroxides content (in Polish).
  • 18. Ponne C.T., Moller A.C., Tijskens L.M.M., Bartels P.V., Meijer M.M.T., Influence of microwave and steam heating on lipase activity and microstructure of rapeseed (Brassica napus L.). J. Agric. Food Chem., 1996, 44, 2824–2828.
  • 19. Rodriguez-Rosales M.P., Kerkeb L., Ferrol N., Donaire J.P., Lipoxygenase activity and lipid composition of cotyledons and oil bodies of two sunflower hybrids. Plant Physiol. Biochem., 1998, 36, 285–291.
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  • 22. Surrey K., Spectrophotometric method for determination of lipoxydase activity. Plant Physiol., 1964, 30, 65–70.
  • 23. Watanabe K., Uno T., Koshiba T., Okamoto T., Ebara Y., Ueji S., How does lipase flexibility affect its enantioselectivity in organic solvents? A possible role of CH center dot center dot center dot pi association in stabilization of enzyme-substrate complex. Bull. Chem. Soc. Jap., 2004, 77, 543–548.
  • 24. Yang L.R., Luo J.X., Wu J.P., Tang S.P., Lipase-catalyzed alcoholysis of alpha-cyano-3-phenoxybenzyl acetate in organic media. Chinese. J. Organic Chem., 2003, 23, 1260–1263.
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  • 26. Zimmerman N.D., Vick B.A., Hydroperoxide isomerase. A new enzyme in lipid metabolism. Plant Physiol., 1970, 46, 445–453.

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Bibliografia

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