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2015 | 37 | 11 |
Tytuł artykułu

Modulation of viral infection in plants by exogenous guanosine

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
To evaluate the role of purines in antiviral treatments in plants, ribavirin (RB) and tiazofurin (TZ) were applied in combination with guanosine (GS) or adenosine (AS) in in vitro grapevine or tobacco explants infected by Grapevine leafroll associated virus 3 (GLRaV-3) and Cucumber mosaic virus (CMV), respectively. Using a microelectrochemical (trans-plasma membrane electron transport, t-PMET) technique, in vivo assay of free reduced nicotinamide adenine dinucleotide (NADH) was also carried out to estimate the inosine monophosphate dehydrogenase inhibition caused by drugs. Antiviral effectiveness of TZ, evaluated as virus-free explants or virus copies, was significantly hindered by GS in both species, while AS did not interfere with the drugs. GS, but not AS, slightly hindered the antiviral effectiveness of RB. With regard to NADH tests, t-PMET inhibition caused by RB and TZ was dose dependent and the interference of drugs with the NAD+/NADH conversion was confirmed by NADH content. Findings indicate that exogenous GS up to 0.50 mM replenished the GS pool depleted by drugs, contrasting antiviral action. At higher doses of GS, the TZ antiviral action was completely inhibited and exogenous GS caused a feedback that reduced t-PMET activity. The reversal was partially against RB, suggesting that the reduction of the GS pool contributed to the antiviral activity of RB, but it was not the only cause of antiviral effectiveness.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
11
Opis fizyczny
Article: 226 [6 p.], fig.,ref.
Twórcy
autor
  • Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, 80, 56124, Pisa, Italy
  • Department of Agrifood Production and Environmental Sciences, sect. Arboriculture, Laboratory of Electrophysiology, University of Florence, Viale delle Idee, 30, 50019, Sesto Fiorentino, Florence, Italy
autor
  • Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, 80, 56124, Pisa, Italy
autor
  • Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, 80, 56124, Pisa, Italy
Bibliografia
  • Allison AC, Eugui EM (2000) Mycophenolate mofetil and its mechanisms of action. Immunopharmacology 47:85–118
  • Bertolini E, Olmos A, López MM, Cambra M (2003) Multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four RNA viruses and Pseudomonas savastanoi pv. savastanoi in olive trees. Phytopathology 93:286–292
  • Cabaleiro C, Couceiro C, Pereira S, Cid M, Barrasa M, Segura A (2008) Spatial analysis of epidemics of Grapevine leafroll associated virus-3. Eur J Plant Pathol 121:121–130
  • Del Principe D, Avigliano L, Savini I, Catani MV (2011) Transplasma membrane electron transport in mammals: functional significance in health and disease. Antioxid Redox Sign 14:2289–2318
  • Feng JL, Che SN, Tang XS, Ding XF, Du ZY, Chen JS (2006) Quantitative determination of cucumber mosaic virus genome RNAs in virions by real-time reverse transcription-polymerase chain reaction. Acta Bioch Bioph Sin 38:669–676
  • Franchetti P, Capellacci L, Grifantini M (1996) IMP dehydrogenase as a target of antitumor and antiviral chemotherapy. Il Farmaco 51:457–469
  • Glesne DA, Collart FR, Huberman E (1991) Regulation of IMP dehydrogenase gene expression by its end products, guanine nucleotides. Mol Cell Biol 11:5417–5425
  • Guazzelli L, D’Andrea F, Giorgelli F, Catelani G, Panattoni A, Luvisi A (2015) Synthesis of PAMAM dendrimers loaded with mycophenolic acid to be studied as new potential immunosuppressants. J Chem 2015. doi:10.1155/2015/263072
  • Guta IC, Buciumeanu EC, Gheorghe RN, Teodorescu A (2010) Solutions to eliminate grapevine leafroll associated virus serotype 1 + 3 from V. vinifera L. cv. Ranai Magaraci. Rom Biotech Lett 15:72–78
  • Luvisi A, Rinaldelli E, Panattoni A, Triolo E (2012) Membrane transport of antiviral drugs in plants: an electrophysiologicalstudy in grapevine explants infected by Grapevine leafroll associated virus 1. Acta Physiol Plant 34:2115–2123
  • MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain Reaction. Plant Dis 81:222–226
  • Markland W, McQuaid TJ, Jain J, Kwong AD (2000) Broadspectrum antiviral activity of the IMP dehydrogenase inhibitor VX-497: a comparison with ribavirin and demonstration of antiviral additivity with alpha interferon. Antimicrob Agents Chemother 44:859–866
  • Mitsuhashi S, Takenaka J, Iwamori K, Nakajima N, Ubukat M (2010) Structure–activity relationships for inhibition of inosine monophosphate dehydrogenase and differentiation induction of K562 cells among the mycophenolic acid derivatives. Bioorgan Med Chem 18:8106–8111
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nascimento LC, Pio Ribeiro G, Willadino L, Andrade GP (2003) Stock indexing and Potato Virus Y elimination from potato plants cultivated in vitro. Sci Agric 60:525–530
  • Nouri K, Yazdanparast R, Sarafnejad A (2011) Guanosine supplementation reduces the antiproliferative and apoptotic effects of the IMPDH inhibitor gnidilatimonoein in K562 cells. Cell Biol Int 35:1001–1008
  • Panattoni A, D’Anna F, Triolo E (2007) Antiviral activity of tiazofurin and mycophenolic acid against Grapevine Leafroll associated Virus 3 in Vitis vinifera explants. Antivir Res 73:206–211
  • Panattoni A, Luvisi A, Triolo E (2013a) Elimination of viruses in plants: twenty years of progress. Span J Agric Res 11:173–188
  • Panattoni A, Rinaldelli E, Triolo E, Luvisi A (2013b) In vivo inhibition of trans-plasma membrane electron transport by antiviral drugs in grapevine. J Membr Biol 246:513–518
  • Panattoni A, Luvisi A, Fuselli S, D’Andrea F, Giorgelli F, Guazzelli L, Catelani G, Triolo E (2014) Antiviral activity of mycophenolic acid derivatives in plants. Acta Virol 58:99–102
  • Petrelli R, Vita P, Torquati I, Felczak K, Wilson DJ, Franchetti P, Cappellacci L (2013) Novel inhibitors of Inosine monophosphate dehydrogenase in patent literature of the last decade. Recent Pat Anti-Cancer 8:1–23
  • Quoirin M, Lepoivre P (1977) Etude de millieux adaptes aux cultures in vitro de prunus. Acta Hortic 78:437–442
  • Rinaldelli E, Panattoni A, Luvisi A, Triolo E (2012) Effect of mycophenolic acid on trans-plasma membrane electron transport and electric potential in virus-infected plant tissue. Plant Physiol Bioch 60:137–140
  • Sepúlveda CS, García CC, Fascio ML, D’Accorso NB, Docampo Palacios ML, Pellón RF, Damonte EB (2012) Inhibition of Junin virus RNA synthesis by an antiviral acridone derivative. Antivir Res 9:16–22
  • Skiada FG, Maliogka VI, Katis NI, Eleftheriou EP (2013) Elimination of Grapevine rupestris stem pitting-associated virus (GRSPaV) from two Vitis vinifera cultivars by in vitro chemotherapy. Eur J Plant Pathol 135:407–414
  • Takhampunya R, Ubol S, Houng HS, Cameron CE, Padmanabhan R (2006) Inhibition of dengue virus replication by mycophenolic acid and ribavirin. J Gen Virol 87:1947–1952
  • Taylor AR, Chow RH (2001) A microelectrochemical technique to measure transplasma membrane electron transport in plant tissue and cells in vivo. Plant Cell Environ 24:749–754
  • Tsai CW, Daugherty MP, Almeida RPP (2013) Seasonal dynamics and virus translocation of Grapevine leafroll-associated virus 3 in grapevine cultivars. Plant Pathol 61:977–985
  • Turturo C, Saldarelli P, Yafeng D, Digiaro M, Minafra A, Savino V, Martelli GP (2005) Genetic variability and population structure of grapevine leafroll–associated virus 3 isolates. J Gen Virol 86:217–224
  • Velasco L, Bota J, Montero R, Cretazzo E (2014) Differences of three Ampeloviruses’ multiplication in plant may explain their incidences in vineyards. Plant Dis 98:395–400
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-3ed9876e-d4a0-437c-a497-b804e4226310
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