The application of impedance microsensors for real-time analysis of Pseudomonas aeruginosa biofilm formation

• Autorzy: Chabowski K. , Junka A.F. , Szymczyk P. , Piasecki T. , Sierakowskii A. , Maczynska B. , Nitsh K.
• Języki publikacji: EN

Abstrakty

EN

Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates the ability of impedance microsensors to detect Pseudomonas aeruginosa biofilm presence using the impedance spectroscopy method. The measured data were analyzed using Electrical Equivalent Circuit modelling (EEC). It allowed to recognize conduction and polarization phenomena on the sensors surface and in its environment. The impedance assay results, confirmed by means of electron microscopy and quantitative cultures, indicate that specific EEC parameters may be used for monitoring the development of pseudomonal biofilm.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2015
• Tom: 64
• Numer: 2
• Opis fizyczny: p.115-120,fig.,ref.

Twórcy

autor

Chabowski K.

• Faculty of Microsystem Electronics and Photonics, Wroclaw University of Technology, Wroclaw, Poland

autor

Junka A.F.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, Wroclaw, Poland

autor

Szymczyk P.

• Centre for Advanced Manufacturing Technologies, Wroclaw University of Technology, Wroclaw, Poland

autor

Piasecki T.

• Faculty of Microsystem Electronics and Photonics, Wroclaw University of Technology, Wroclaw, Poland

autor

Sierakowskii A.

• Division of Silicon Microsystem and Nanostructure Technology, Institute of Electron Technology, Warsaw, Poland

autor

Maczynska B.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, Wroclaw, Poland

autor

Nitsh K.

• Faculty of Microsystem Electronics and Photonics, Wroclaw University of Technology, Wroclaw, Poland

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