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2014 | 36 | 03 |

Tytuł artykułu

Inhibition of germination and early growth of rape seed (Brassica napus L.) by MCPA in anionic and ester form

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Języki publikacji



MCPA (4-chloro-2-methylphenoxy) acetic acid is a common synthetic auxin used as a herbicide. The purpose of this study was to determine the effects of four new forms of MCPA being the herbicidal ionic liquids (HILs) with MCPA as an anion and two previously known formulations (potassium–sodium salt and 2-ethylhexyl ester) on seed germination and seedling development of winter oilseed rape (Brassica napus). Rape plants are susceptible to MCPA and volunteers can be a big problem in crop rotation. Seedling fresh weight and root length were quantified, mitotic activity, as well as lipid, starch, hydrogen peroxide and polyphenol contents were assessed by light and fluorescence microscopy and the computer-aided cytophotometer. In primary roots mitotic activity was almost completely inhibited under the influence of herbicides, cell elongation zones and root hair zones were significantly reduced, and a characteristic bolded root segment formed just above a meristem. In contrast to the traditional salt formulation the new HILs were weak inducers of hydrogen peroxide synthesis, but were potent stimulators of the synthesis of phenolic compounds and storage as well as emergency substances such as lipids and starch. All tested forms of MCPA caused strong phytotoxic effect on winter rape seedlings, but the tested HILs were more effective.

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Opis fizyczny



  • Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland
  • Institute of Plant Protection, National Research Institute, Wl. Wegorka 20, 60-318, Poznan, Poland
  • Poznan University of Technology, Sklodowskiej-Curie 2, 60-965, Poznan, Poland
  • Agronomy Department, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznan, Poland
  • Institute of Plant Protection, National Research Institute, Wl. Wegorka 20, 60-318, Poznan, Poland
  • Agronomy Department, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznan, Poland


  • Campanoni P, Nick P (2005) Auxin-dependent cell division and cell elongation. 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid activate different pathways. Plant Physiol 137:939–948
  • Cheng Y, Song C (2006) Hydrogen peroxide homeostasis and signaling in plant cells. Sci China Ser C Life Sci 49:1–11
  • Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Bresugem F (2000) Dual action of the active oxygen species during plant stress response. Cell Mol Life Sci 57:779–795
  • DiTomaso JM (1999) Barriers to foliar penetration and uptake of herbicides. Proc Calif Weed Sci Soc 51:150–155
  • Dunand Ch, Crèvecoeur M, Penel C (2007) Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases. New Phytol 174:332–341
  • Faivre-Rampant O, Charpentier J-P, Kevers C, Dommes J, Van Onckelen H, Jay-Allemand C, Gaspar T (2002) Cuttings of the non-rooting rac tobacco mutant overaccumulate phenolic compounds. Funct Plant Biol 29:63–71
  • Flessner ML, Dute RR, McElroy JS (2011) Anatomical response of St. Augustine grass to aminocyclopyrachlor treatment. Weed Sci 59:263–269
  • Foreman J, Demidchik V, Bothwell JHF, Mylona P, Mledema H, Torres MA, Linstead P, Costa S, Brownlee C, Jones JDG, Davies JM, Dolan L (2003) Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature 422:442–446
  • Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345
  • Gechev TS, Hille J (2005) Hydrogen peroxide as a signal controlling plant programmed cell death. J Cell Biol 168:17–20
  • Grossmann K (2000) The mode of action of quinclorac: a case study of a new auxin-type herbicide. In: Cobb AH, Kirkwood RC (eds) Herbicides and their mechanisms of action. Sheffield Academic Press, Sheffield, pp 181–214
  • Grossmann K (2010) Auxin herbicides: current status of mechanism and mode of action. Pest Manag Sci 66:113–120
  • Grossmann K, Kwiatkowski J, Tresch S (2001) Auxin herbicides induce H₂O₂ overproduction and tissue damage in cleavers (Galium aparine L.). J Exp Bot 52:1811–1816
  • Hough WL, Rogers RD (2007) Ionic liquids then and now: from solvents to materials to active pharmaceutical ingredients. Bull Chem Soc Jpn 80:2262–2269
  • Hough WL, Smiglak M, Rodríguez H, Swatloski RP, Spear SK, Daly DT, Pernak J, Grisel JE, Carliss RD, Soutullo DM, Davis JH, Rogers RD (2007) The third evolution of ionic liquids: active pharmaceutical ingredients. New J Chem 31:1429–1436
  • Jin ZL, Zhang F, Ahmed ZI, Rasheed M, Naeem MS, Ye QF, Zhou WJ (2010) Differential morphological and physiological responses of two oilseed Brassica species to a new herbicide ZJ0273 used in rapeseed fields. Pestic Biochem Physiol 98:1–8
  • Kirchner B (2009) Ionic liquids. Springer, Berlin
  • Kumar S, Arya SK, Roy BK, Singh AK (2010) The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turk J Biol 34:55–66
  • Kwak JM, Nguyen V, Schroeder JI (2006) The role of reactive oxygen species in hormonal responses. Plant Physiol 141:323–329
  • Lee TT (1977) Role of phenolic inhibitors in peroxidase-mediated degradation of indole-3-acetic acid. Plant Physiol 59:372–375
  • Maszewski J, Kaźmierczak A (1998) Repression of genetic activity in root meristem cells by peptidic factor derived from male sex organs of Chara. Biol Plant 41:357–368
  • Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530
  • Mithila J, Hall JC, Johnson WG, Kelley KB, Riechers DE (2011) Evolution of resistance to auxinic herbicides: historical perspectives, mechanisms of resistance, and implications for broadleaf weed management in agronomic crops. Weed Sci 59:445–457
  • Mittler R, Vanderauwera S, Gollery M, van Breugesem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498
  • Monaco TJ, Weller SC, Ashton FM (2002) Weed science: principles and practices. Wiley-Blackwell, New York
  • Naeem MS, Rasheed M, Liu D, Jin ZL, Ming DF, Yoneyama K, Takeuchi Y, Zhou WJ (2011) 5-Aminolevulinic acid ameliorates salinity-induced metabolic, water-related and biochemical changes in Brassica napus L. Acta Physiol Plant 33:517–528
  • Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002) Hydrogen peroxide and nitric oxide as signaling molecules in plants. J Exp Bot 53:1237–1247
  • Olivier-Bourbigou H, Magna L, Morvan D (2010) Ionic liquids and catalysis: recent progress from knowledge to applications. Appl Catal A 373:1–56
  • Passardi F, Penel C, Dunand Ch (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9:534–540
  • Passardi F, Tognolli M, De Meyer M, Penel C, Dunand Ch (2006) Two cell wall associated peroxidases from Arabidopsis influence root elongation. Planta 223:965–974
  • Pernak J, Syguda A, Janiszewska D, Materna K, Praczyk T (2011) Ionic liquids with herbicidal anions. Tetrahedron 67:4838–4844
  • Plechkova NV, Seddon KR (2008) Applications of ionic liquids in the chemical industry. Chem Soc Rev 37:123–150
  • Polit JT, Maszewski J, Kaźmierczak A (2003) Effect of BAP and IAA on the expression of G1 and G2 control points and G1-S and G2-M transitions in root meristem cells of Vicia faba. Cell Biol Int 27:559–566
  • Praczyk T, Kardasz P, Jakubiak E, Syguda A, Materna K, Pernak J (2012) Herbicidal ionic liquids with 2,4-D. Weed Sci 60:189–192
  • Riechers DE, Wax LM, Liebl RA, Bush DR (1994) Surfactant-increased glyphosate uptake into plasma membrane vesicles isolated from common lambsquarters leaves. Plant Physiol 105:1419–1425
  • Rogers RD, Seddon KR (2003a) Ionic liquids as green solvents: progress and prospects. American Chemical Society, Washington DC Rogers RD, Seddon KR (2003b) Ionic liquids—solvents of the future? Science 302:792–793
  • Schnablová R, Synková H, Vičánková A, Burketová L, Eder J, Cvikrová M (2006) Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth. Plant Physiol Biochem 44:526–534
  • Schoenwaelder MEA (2008) The biology of phenolic containing vesicles. Algae 23:163–175
  • Senseman SA (2007) Herbicide handbook. Weed Science Society of America, Lawrence
  • Sikka K, Sharma AK (1976) The effects of some herbicides on plant chromosomes. Proc Indian Natl Sci Acad 42:299–307
  • Šimonovičová M, Huttová J, Mistrík I, Široká B, Tamás L (2004) Peroxidase mediated hydrogen peroxide production in barley roots grown under stress conditions. Plant Growth Regul 44:267–275
  • Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J 11:1187–1194
  • Tsugeki R, Ditengou FA, Sumi Y, Teale W, Palme K, Okada K (2009) NO VEIN mediates auxin-dependent specification and patterning in the Arabidopsis embryo, shoot, and root. Plant Cell 21:3133–3151
  • Volpert R, Osswald W, Elstner EF (1995) Effects of cinnamic acid derivatives on indole acetic acid oxidation by peroxidase. Phytochemistry 38:19–22
  • Vranová E, Inzé D, Van Brensegem F (2002) Signal transduction during oxidative stress. J Exp Bot 53:1227–1236
  • Wasserscheid P, Welton T (2008) Ionic liquids in synthesis. Wiley, Weinheim
  • Zhang WF, Zhang F, Raziuddin R, Gong HJ, Yang ZM, Lu L, Ye QF, Zhou WJ (2008) Effects of 5-aminolevulinic acid on oilseed rape seedling growth under herbicide toxicity stress. J Plant Growth Regul 27:159–169
  • Zsoldos F, Haunold E (1979) Effects of pH changes on ion and 2,4-D uptake of wheat roots. Physiol Plant 47:77–80

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