Modified method of Hypoderma bovis proteins transfer obtained from gel by native electrophoresis onto nitrocellulose membrane

• Autorzy: Cencek T. , Karamon J. , Sroka J. , Zdybel J.
• Języki publikacji: EN

Abstrakty

EN

This article presents the essential stages leading to development of a modified method for Hypoderma bovis protein transfer, and estimation of the usefulness of semi-quantitative densitometric analysis of western-blottig results. The principal of the method was to incubate the gel obtained with the native electrophoresis method in buffer containing SDS prior to transfer of the separated proteins of L1 H. bovis onto nitrocellulose membrane. In two experiments, the authors estimated the efficacy of the new method and the possibility of using this method for semi-quantitative densitometric investigations.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 4
• Opis fizyczny: p.547-552,fig.,ref.

Twórcy

autor

Cencek T.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Karamon J.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Sroka J.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zdybel J.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Baldelli B., Polidori G.A., Grelloni V., Principato M., Moretti A., Fioretti D P., Piergili Fioretti D.: The ELISA method for the detection of antibodies in bovines affected by Hypoderma infestation: preliminary results. Parassitologia 1983, 23, 115-118.
• 2. Boulard C., Villejoubert C.: Use of pooled serum or milk samples for the epidemiological surveillance of bovine hypodermosis. Vet Parasitol 1991, 39, 171-183.
• 3. Casserly U., Mooney M.T., Troy D.: Standarisation and application of semi-quantitative SDS-PAGE method for measurement of myofibrillar fragments in bovine longissimus muscle. Food Chem 2000, 69, 379-385.
• 4. Cencek T., Influence of enzymatic activity of Hypoderma bovis crude antigen proteins on the immunogenicity of the antigen. Thesis. National Veterinary Research Institute 2006, Pulawy, Poland.
• 5. Cencek T.: Thermostability of Hypoderma bovis antigen. Bull Vet Inst Pulawy 2003, 47, 121-131.
• 6. Davis B.J.: Disc electrophoresis II. Method and application to human serum proteins. Ann New York Acad Sci 1964, 121, 404-427.
• 7. Khan M.A., Connell R., Darcel C.: Immunization and parenteral chemotherapy for the control of cattle grubs Hypoderma lineatum (De Vill.) and H. bovis (L.) in cattle. Can J Comp Med 1960, 24, 177-180.
• 8. Khyse-Andersen J.: Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from Polyacrylamide to nitrocellulose. J Biochem Biophys Meth 1984, 10, 203-209.
• 9. Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 580-685.
• 10. Lecroisey A., Tong N.T., Keil B.: Hypodermin B, a trypsin-related enzyme from the insect Hypoderma lineatum. Comparison with hypodermin A and Hypoderma collagenase, two serine proteinases from the same source. Eur J Biochem 1983, 134, 261-267.
• 11. Magat A., Boulard C.: Essais de vaccination contre l'hypodermose bovine avec un vaccine contenant une collagenase brute extradite des larves de 1er stade ďHypoderma lineatum. C R Acad. Sci. Hebd. Seances Acad Sci D 1970, 207, 728-730.
• 12. Malassagne B., Regimbeau J.M., Taboit F., Troalen F., Chereau C., Moire N., Attal J., Batteux F., Conti F., Calmus Y., Houssin D., Boulard C., Houdebine L.M., Weill B.: Hypodermin A, a new inhibitor of human complement for the prevention of xenogeneic hyperacute rejection. Xenotransplantation 2003,10, 267-277.
• 13. Ornstein L.: Disc electrophoresis I. Background and theory. Ann New York Acad Sci 1964, 121, 321-349.
• 14. Otranto D.: The immunology of myiasis: parasite survival and host defense strategies. Trends Parasitol 2001, 17, 176-182.
• 15. Planas A.M., Sole S., Justicia C., Farre E.R.: Estimation of gelatinase content in rat brain: effect of focal ischemia. Biochem Biophys Res Commun 2000, 278, 803-807.
• 16. Pruett J.H., Barrett C.C.: Induction of intradermal skin reactions in the bovine by fractionated proteins of Hypoderma lineatum. Vet Parasitol 1984, 16, 137-146.
• 17. Pruett J H., Scholl P.J., Temeyer K.B.: Shared epitopes between the soluble proteins of Hypoderma lineatum and Hypoderma bovis first instars. J Parasitol 1990, 76, 881- 888.
• 18. Pruett J.H., Temeyer K.B., Burkett B.K.: Antigenicity and immunogenicity of Hypoderma lineatum soluble proteins in the bovine host. Vet Parasitol 1988, 29, 53-63.
• 19. Ratajska A., Cleutjens J.P.M.: Embryogenesis of rat heart: the expression of collagenases. Basic Res Cardiol 2002, 97, 189-197.
• 20. Regimbeau J.M., Malassagne B., Taboit F., Boulard C., Houssin D., Weill B.: Mise au point d'un modele de coeur isole perfuse. Application a l'etude de l'hypodermine A dans la prevention du rejet suraigu xenogenique. Ann Chir 2001, 126, 1007-1015.
• 21. Robertson R.H.: Potential for vaccines as a protection against cattle grubs. Research highlights, Lethbridge Research Station, Agriculture Canada, 1978, pp. 46-47.
• 22. Sinclair I. J., Wassail D.A.: Enzyme-linked immunosorbent assay for the detection of antibodies to Hypoderma bovis in cattle. Res Vet Sci 1983, 34, 251- 252.
• 23. Stahl D., Hoemberg M., Cassens U., Pachmann U., Sibrowski W.: Influence of isotypes of disease-associated autoantibodies on the expression of natural autoantibody repertoires in humans. Immunol Lett 2006, 102, 50-59.
• 24. Stahl D., Lacroix-Desmazes S., Mouthon L., Kaveri S.V., Kazatchkine M.D.: Analysis of human self-reactive antibody repertoires by quantitative immunoblotting. J Immunol Meth 2000, 240, 1-14.
• 25. Temeyer K.B., Pruett J.H., Beetham P.K.: Production of monoclonal antibodies specific for hypodermin A, a serine protease of the common cattle grub, Hypoderma lineatum. Southwest Entomol 1998, 23, 229-235.