In vitro adventitious shoots regeneration from ligulate florets in the espect of application in Chrysanthemum breeding

• Autorzy: Tymoszuk A. , Zalewska M.

Warianty tytułu

PL

Regeneracja in vitro pędów przybyszowych z kwiatów języczkowatych w aspekcie zastosowania w hodowli chryzantemy

• Języki publikacji: EN

Abstrakty

EN

Chrysanthemum mutants can be chimeras. The regeneration in vitro of adventitious shoots from ligulate florets can lead to the separation of chimera components, resulting in producing a new cultivar. There was determined the effect of various factors on the number and length of shoots regenerating in vitro from ligulate florets of Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’. The ligulate florets were inoculated on the MS [1962] medium supplemented with cytokinin (0; 4.44; 8.88; 13.32; 22.20 μM·dm-3 BAP; 4.65; 23.23; 46.47; 69.70 μM·dm-3 KIN) and auxin (0; 0.54; 1.08; 1.61; 2.69 μM·dm-3 NAA). Most shoots regenerate when 8.88; 13.32 μM·dm-3 BAP or 69.70 μM·dm-3 KIN and 2.69 μM·dm-3 NAA or 8.88 μM·dm-3 BAP and 1.61 μM·dm-3 NAA are supplemented. Adding 0.29; 1.44 or 2.89 μM·dm-3 GA3 to the MS medium with 8.88 μM·dm-3 BAP and 2.69 μM·dm-3 NAA limits the shoot regeneration efficiency and does not stimulate their elongation. An increase in the shoot number and length is affected by the subculture of regenerating ligulate florets from the MS medium containing 8.88 μM·dm-3 BAP and 2.69 μM·dm-3 NAA on the medium with 2.89 μM·dm-3 GA3 and 2.69 μM·dm-3 NAA. There were found no differences in the number and length of shoots regenerating on ligulate florets inoculated on solid or in liquid MS medium with 8.88 μM·dm-3 BAP and 2.69 μM·dm-3 NAA. The subculture of regenerating ligulate florets from the solid into liquid medium increases the number of regenerating shoots and stimulates their elongation growth, however these shoots are deformed.

PL

Mutanty chryzantem mogą był chimerami. Regeneracja in vitro pędów przybyszowych z kwiatów języczkowatych może doprowadzić do rozdzielenia komponentów składowych chimery i w rezultacie do uzyskania nowej odmiany. W badaniach określono wpływ różnych czynników na liczbę i długość pędów regenerujących in vitro z kwiatów języczkowatych Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’. Kwiaty języczkowate inokulowano na pożywkę MS [1962] uzupełnioną cytokininą (0; 4,44; 8,88; 13,32; 22,20 μM·dm-3 BAP; 4,65; 23,23; 46,47; 69,70 μM·dm-3 KIN) oraz auksyną (0; 0,54; 1,08; 1,61; 2,69 μM·dm-3 NAA). Stwierdzono, Īe najwięcej pędów regeneruje przy dodatku do pożywki 8,88; 13,32 μM·dm-3 BAP lub 69,70 μM·dm-3 KIN i 2,69 μM·dm-3 NAA albo 8,88 μM·dm-3 BAP i 1,61 μM·dm-3 NAA. Dodatek 0,29; 1,44 lub 2,89 μM·dm-3 GA3 do pożywki MS z 8,88 μM·dm-3 BAP i 2,69 μM·dm-3 NAA ogranicza wydajność regeneracji pędów i nie stymuluje ich elongacji. Na zwiększenie liczby i długości pędów wpływa przeniesienie regenerujących kwiatów języczkowatych z poĪywki MS zawierającej 8,88 μM·dm-3 BAP i 2,69 μM·dm-3 NAA na pożywka z 2,89 μM·dm-3 GA3 i 2,69 μM·dm-3 NAA. Nie stwierdzono różnic w liczbie i długości pędów powstających na kwiatach języczkowatych umieszczonych na stałej lub w płynnej pożywce MS z 8,88 μM·dm-3 BAP i 2,69 μM·dm-3 NAA. Przeniesienie regenerujących kwiatów języczkowatych z pożywki stałej do płynnej zwiększa liczbę regenerujących pędów i stymuluje ich wzrost elongacyjny. Pędy te jednak są zdeformowane.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 2
• Opis fizyczny: p.45-58,fig.,ref.

Twórcy

autor

Tymoszuk A.

• Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, University of Technology and Life Science, Bernardyska 6, 85-029 Bydgoszcz, Poland

autor

Zalewska M.

• University of Technology and Life Science, Bydgoszcz, Poland,

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