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2012 | 54 | 1 |

Tytuł artykułu

Morphological and molecular differentiation between Egyptian species of Pancratium L. (Amaryllidaceae)

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EN

Abstrakty

EN
Three problems in the taxonomy of Pancratium in Egypt are the lack of publications, a lack of clarity about the relationships between recently distinguished species, and the lack of markers for examining the levels and patterns of variation in rare and endemic species; the latter hinders work in plant conservation genetics. In this study we reassessed the taxonomic status of the Pancratium species of Egypt, and examined morphological and genetic variation within and between species, using specimens from different populations collected throughout its distribution range in the country. Our assessment was based on 38 macromorphological characters mainly representing vegetative parts, flowers, fruits and seeds, in addition to RAPD data. The results revealed five morphologically distinguished Pancratium species in Egypt, of which P. trianthum Herb. is newly recorded. Species identification was confirmed by two phenetic dendrograms generated with 26 quantitative morphological characters and RAPD data, while species delimitation was verified by principal component analysis. The diagnostic floral characters are those of the perianth, corona teeth, pistil, stamens, aerial scape, spathe, and number of flowers. The retrieved RAPD polymorphic bands show better resolution of the morphologically and ecologically closely allied Pancratium species (P. arabicum and P. maritimum), and also confirm the morphological and ecological divergence of P. tortuosum from the other studied species. These results are supported by the constructed UPGMA dendrogram.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

54

Numer

1

Opis fizyczny

p.53-64,fig.,ref.

Twórcy

autor
  • The Herbarium, Faculty of Science, Cairo University, Giza 12613, Egypt
autor
autor

Bibliografia

  • ANDERBERG MR. 1973. Cluster Analysis for Applications. Monographs and Textbooks on Probability andMathematical Statistics. Academic Press Inc., NewYork.
  • ANDREWS FW. 1956. The Flowering Plants of the Sudan, vol. 3. Arbroath, Scotland.
  • APG III. 2009. An update of the Angiosperm phylogeny group classification for the orders and families of floweringplants: APG III. Botanical Journal of the LinneanSociety 161: 105–121.
  • BAKER JG. 1898. Pancratium L. In: Thistelton-Dyer WT [ed.], Flora of Tropical Africa, vol. 7, 406–407. L. Reeve andCo. Ltd., Ashford, Kent.
  • BJÖRNSTAD IN. 1973. A revision of the genus Pancratium in Africa south of the Sahara. Norwegian Journal of Botany 20 (4): 281–291.BOULOS L. 2005. Flora of Egypt. Alismataceae toOrchidaceae, vol. 4. Al Hadara Publishing, Cairo, Egypt.
  • BRUMMITT RK, and POWEL CE. 2001. Authors of Plant Names. Royal Botanic Gardens, Kew.
  • DINSMORE JE. 1933. In: Post GE [ed.]. Flora of Syria, Palestine and Sinai. 2nd ed., vol. 2. American Press,Beirut.
  • DOYLE JJ, and DOYLE JL. 1990. Isolation of plant DNA from fresh tissues. Focus 12: 13–15.
  • EL-GADI A. 1978. Amaryllidaceae In: Jafri SMH, and EL-Gadia [eds.], Flora of Libya, vol. 51, 1–5. Al Faateh University,Flora Committee, Tripoli.
  • EL-HADIDY A, ABD EL-GHANI M, AMER W, and HASSAN R. 2011. Systematic revision of the genus Pancratium L.(Amaryllidaceae) in Egypt with a new addition. NotulaeScientia Biologicae 3(2): 24–38.
  • FEINBRUN-DOTHAN N. 1986. In: Zohary M [ed.], Flora Palaestina, vol. 4. Israel Academy of Sciences and Humanities, Jerusalem.
  • GRASSI F, CAZZANIGA E, MINUTO L, PECCENINI S, BARBERIS G, and BASSO B. 2005. Evaluation of biodiversity and conservationstrategies in Pancratium maritimum L. for theNorthern Tyrrhenian Sea. Biodiversity andConservation 14 (9): 2159–2169.
  • HELLER D, and HEYN CC. 1991. An Annotated catalogue of the Flora of the Middle East. Conspectus Florae Orientalis 6: 53. Israel Academy of Sciences and Humanities, Jerusalem.
  • HOLMGREN PK, HOLMGREN NH, and BARNETT LC 1990. Index Herbariorum. 8th ed., vol. 1. New York Botanical Gardens, New York, U.S.A.
  • IOSET JR, MARSTON A, MAHABIR, PG, and HOSTETTMANN K. 2001. A methylflavan with free radical scavenging propertiesfrom Pancratium littorale. Fitoterapia 72: 35–39.
  • KARB A, SEBERG O, and BUIATTI M. 1996. Molecular techniques in the assessment of botanical diversity. Annals ofBotany 78: 143–149.
  • KOVACH WL. 1999. MVSP – A Multivariate Statistical Package for Windows, version 3.1. Kovach Computing Services,Pentraeth, Wales, U.K.
  • KRESOVICH S, WILLIAMS JGK, MCFERSON JR, ROUTMAN EJ, and SCHAAL BA. 1992. Characterization of genetic identitiesand relationships of Brassica oleracea L. via a randomamplified polymorphic DNA assay. Theoretical andApplied Genetics 85: 190–196.
  • LI YC, FAHIMA T, BEILES A, KOROL AB, and NEVO E. 1999. Microclimatic stress differentiation in wild emmer wheat, Triticum dicoccoides. Theoretical and Applied Genetics 98: 873–883.
  • MABBERLEY DJ. 1993. The Plant Book. Cambridge University Press, Cambridge. MEEROW AW, GUY CL, Li Q-B, and CLAYTON JR. 2002. Phylogeny of the tribe Hymenocallideae (Amaryllidaceae) based on morphology and molecular characters. Annalsof Missouri Botanical Gardens 89(3): 400–413.
  • MAIRE R. 1959. Flore de l'Afrique du Nord. Monocotyledonae, vol. 6, 33–41. Lechevalier, Paris. MANTEL N. 1967. The detection of disease clustering and generalizedregression approach. Cancer Research 27:209–220.
  • MEIKLE RD. 1985. Flora of Cyprus, vol. 2. Bentham-Moxon Thrust, Royal Botanic Gardens, Kew. MORTON JK. 1965. The experimental taxonomy of the West African species of Pancratium L. (Amaryllidaceae). KewBulletin 19 (2): 337–347.
  • NEI M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics89: 583–590.
  • POREBSKY S, GRANT-BAILEY L, and BAUM BR. 1997. Modification of a CTAB DNA extraction protocol for plants containinghigh polysaccharide and polyphenol components. PlantMolecular Biology Reporter 15: 8–15.
  • ROHLF FJ. 1998. NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.0. Applied Biosystematics Inc., Exeter Software, New York.
  • SALTZ D, and WARD D. 2000. Responding to a three-pronged attack: desert lilies subject to herbivory by Dorcasgazelles. Plant Ecology 148: 127–138.
  • SNEATH PHA, and SOKAL RR. 1973. Numerical Taxonomy: The principles and Practice of Numerical Classification. W.H. Freeman Press, San Francisco.
  • SOKAL RR, and MICHENER CD. 1958. A statistical method for evaluating systematic relationships. University of Kansas Bulletin 38: 1409–1438.
  • SOKAL RR, and ROHLF FJ. 1995. Biometry. 3rd ed. W. H. Freeman and Company, New York.
  • SOSINSKI B, and DOUCHES DS. 1996. Using polymerase chain reaction based DNA amplification to fingerprint North American potato cultivars. Horticultural Science31:130–133.
  • SÜR-ALTINER D, GÜRKAN E, MUTLU G, TUZLACI E, and ANG Ö. 1999. The antifungal activity of Pancratium maritimum.Fitoterapia 70: 187–189.
  • TÄCKHOLM V. 1974. Students' Flora of Egypt. 2nd ed. Cairo University Press, Beirut.
  • TÄCKHOLM V, and DRAR M. 1954. Flora of Egypt, vol. 3, Bulletin of the Faculty of Science of Cairo University 30:1–638.
  • TRAUB HP. 1963. The Genera of the Amaryllidaceae. The American Plant Life Society, La Jolla, California.
  • WACHIRA FN, WAUGH R, HACKETT CC, and POWELL W. 1995. Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38: 201–210.
  • WIERINGA JJ. 1999. Monopetalanthus exit. A systematic study of Aphanocalyx, Bikinia, Icuria, Michelsonia, and Tetraberlinia (Leguminosae, Caesalpinioideae).Wageningen Agriculture University Papers 99 (4):115–186.
  • WILLIAMS JGK, KUBELIK AR, LIVAK KJ, RAFALSKI JA, and TINGE SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
  • YAMAMOTO I, and DUICH JM. 1994. Electrophoretic identification of cross-pollinated bent grass species and cultivars.Crop Science 34: 792–798.
  • ZAHREDDINE H, CLUBBE C, BAALBAKI R, GHALAYINI A, and TALHOUK SN. 2004. Status of native species in threatenedMediterranean habitats: the case of Pancratium maritimumL. (sea daffodil) in Lebanon. BiologicalConservation 120: 11–18.

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