Evidences of brain and lung invasion of a local water Cryptosporidium parvum isolate in comparison to Iowa strain: serological and immunohistochemical cytokine evaluation

• Autorzy: Gaber M. , Galal L.A.A. , Hassan D. , Badary D.M. , Mohamed I.M.A. , Elossily N.
• Języki publikacji: EN

Abstrakty

EN

Cryptosporidium spp. is an obligate intracellular parasite that has become a community threat. The pathological consequences of cryptosporidiosis vary not only in different Cryptosporidium species but even among different isolates of the same species. The present study aimed to track the serological and immunohistopathological differences between animals infected by Cryptosporidium parvum “Iowa isolate” (CPI) and Cryptosporidium parvum “water isolate” isolated from a local water supply in Assiut Governorate, Egypt (CPW). Three experimental groups were encountered: negative control group (C), the CPI group and the CPW group; each contains ten Swiss albino mice. Serum cytokine: IL10 and TNF-α were measured. Expression of Cryptosporidium antigen and CD3 in the intestinal, pulmonary and brain tissue were evaluated through immunohistochemical assay. IL10 and TNF-α were elevated in both infected groups, over expression of Cryptosporidium protein and CD3 in the intestinal, pulmonary and brain tissue in CPW infected group compared to Iowa infected one. Multi-organs affection occurred in the CPW indicating more severe pathogenicity and virulence than standard Iowa isolate. The local C. parvum isolate was more virulent than tested Iowa isolate as it spread extra-intestinally to reach brain tissue.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2020
• Tom: 66
• Numer: 3
• Opis fizyczny: p.311-318,fig.,ref.

Twórcy

autor

Gaber M.

• Department of Parasitology, Faculty of Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

autor

Galal L.A.A.

• Department of Parasitology, Faculty of Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

autor

Hassan D.

• Department of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

autor

Badary D.M.

• Department of Pathology, Faculty of Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

autor

Mohamed I.M.A.

• Department of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

autor

Elossily N.

• Department of Parasitology, Faculty of Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt

Identyfikatory

DOI

10.17420/ap6603.269